Literature DB >> 35575891

African Swine Fever Virus (ASFV) Indirect ELISA Test Based on the Use of the Soluble Cytoplasmic Semi- purified Antigen (ASFV CP-Ag).

Gallardo Carmina1, R Nieto2, M Arias2.   

Abstract

The present chapter describes a simple and economic indirect enzyme immunoassay (ELISA ) for African swine fever virus (ASFV) antibody detection based on the use of the soluble cytoplasmic fraction of ASFV-infected monkey stable cells (MS). The soluble antigen proteins of ASFV-infected cells are separated by sucrose precipitation centrifugation, and the supernatant above the sucrose layer is used as an ELISA antigen. The test serum sample reacts with the cytoplasmic soluble fraction, and antibodies are detected using a protein A-peroxidase conjugate. This crude antigen is currently recommended as a test reagent in screening and diagnostic tests by the World Organization for Animal Health (OIE).
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  ASFV antibodies; African swine fever virus (ASFV); ELISA; Soluble cytoplasmic antigen

Mesh：

Substances：

Year:  2022        PMID： 35575891     DOI： 10.1007/978-1-0716-2333-6_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Assessment of African Swine Fever Diagnostic Techniques as a Response to the Epidemic Outbreaks in Eastern European Union Countries: How To Improve Surveillance and Control Programs.

Authors:  C Gallardo; R Nieto; A Soler; V Pelayo; J Fernández-Pinero; I Markowska-Daniel; G Pridotkas; I Nurmoja; R Granta; A Simón; C Pérez; E Martín; P Fernández-Pacheco; M Arias
Journal:  J Clin Microbiol       Date:  2015-06-03       Impact factor: 5.948

Review 2.  African swine fever (ASF) diagnosis, an essential tool in the epidemiological investigation.

Authors:  C Gallardo; J Fernández-Pinero; M Arias
Journal:  Virus Res       Date:  2019-07-27       Impact factor: 3.303

3.  Experimental Transmission of African Swine Fever (ASF) Low Virulent Isolate NH/P68 by Surviving Pigs.

Authors:  C Gallardo; A Soler; R Nieto; M A Sánchez; C Martins; V Pelayo; A Carrascosa; Y Revilla; A Simón; V Briones; J M Sánchez-Vizcaíno; M Arias
Journal:  Transbound Emerg Dis       Date:  2015-10-03       Impact factor: 5.005

4.  Comparison of two antigens for use in an enzyme-linked immunosorbent assay to detect African swine fever antibody.

Authors:  M J Pastor; M Arias; J M Escribano
Journal:  Am J Vet Res       Date:  1990-10       Impact factor: 1.156

5.  Antibodies to bovine serum albumin in swine sera: implications for false-positive reactions in the serodiagnosis of African swine fever.

Authors:  J M Escribano; M J Pastor; J M Sánchez-Vizcaíno
Journal:  Am J Vet Res       Date:  1989-07       Impact factor: 1.156

6.  Proteins specified by African swine fever virus: V. Identification of immediate early, early and late proteins.

Authors:  J M Escribano; E Tabarés
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

7.  Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests.

Authors:  C Gallardo; A Soler; R Nieto; A L Carrascosa; G M De Mia; R P Bishop; C Martins; F O Fasina; E Couacy-Hymman; L Heath; V Pelayo; E Martín; A Simón; R Martín; A R Okurut; I Lekolol; E Okoth; M Arias
Journal:  Vet Microbiol       Date:  2012-08-18       Impact factor: 3.293

8.  Proteins specified by African swine fever virus. II. Analysis of proteins in infected cells and antigenic properties.

Authors:  E Tabarés; J Martinez; F Ruiz Gonzalvo; C Sánchez-Botija
Journal:  Arch Virol       Date:  1980       Impact factor: 2.574

9.  African Swine Fever: Lessons to Learn From Past Eradication Experiences. A Systematic Review.

Authors:  Maria Luisa Danzetta; Maria Luisa Marenzoni; Simona Iannetti; Paolo Tizzani; Paolo Calistri; Francesco Feliziani
Journal:  Front Vet Sci       Date:  2020-06-09
  9 in total
  1 in total

1.  Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus.

Authors:  Jingjing Ding; Jifei Yang; Daoyuan Jiang; Yanyang Zhou; Chenxi Li; Yanhua Li
Journal:  Front Cell Infect Microbiol       Date:  2022-09-14       Impact factor: 6.073
  1 in total

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