Literature DB >> 35575889

Quantification of ASFV DNA and RNA in Ornithodoros Soft Ticks.

Rémi Pereira De Oliveira1,2, Laurence Vial3, Marie-Frédérique Le Potier4.   

Abstract

Molecular biology methods are highly sensitive to detect the genome of pathogens and to study their biology. Polymerase chain reaction (PCR) and reverse transcription followed by a polymerase chain reaction (RT-PCR) permit the detection of the presence and the replication of African swine fever virus in soft ticks. Here, we described our techniques to detect and quantify DNA and RNA of African swine fever virus in soft ticks including a housekeeping gene of soft ticks as internal control.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  African swine fever virus; Ornithodoros; PCR; RT-PCR; Soft tick

Mesh：

Substances：

Year:  2022        PMID： 35575889     DOI： 10.1007/978-1-0716-2333-6_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  No evidence of African swine fever virus replication in hard ticks.

Authors:  Helena C de Carvalho Ferreira; Sara Tudela Zúquete; Michiel Wijnveld; Eefke Weesendorp; Frans Jongejan; Arjan Stegeman; Willie L A Loeffen
Journal:  Ticks Tick Borne Dis       Date:  2014-06-26       Impact factor: 3.744

2.  Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.

Authors:  Marylène Tignon; Carmina Gallardo; Carmen Iscaro; Evelyne Hutet; Yves Van der Stede; Denis Kolbasov; Gian Mario De Mia; Marie-Frédérique Le Potier; Richard P Bishop; Marisa Arias; Frank Koenen
Journal:  J Virol Methods       Date:  2011-09-17       Impact factor: 2.014

3.  Molecular diagnosis of African Swine Fever by a new real-time PCR using universal probe library.

Authors:  J Fernández-Pinero; C Gallardo; M Elizalde; A Robles; C Gómez; R Bishop; L Heath; E Couacy-Hymann; F O Fasina; V Pelayo; A Soler; M Arias
Journal:  Transbound Emerg Dis       Date:  2012-03-07       Impact factor: 5.005

4.  Tick-Bacteria Mutualism Depends on B Vitamin Synthesis Pathways.

Authors:  Olivier Duron; Olivier Morel; Valérie Noël; Marie Buysse; Florian Binetruy; Renaud Lancelot; Etienne Loire; Claudine Ménard; Olivier Bouchez; Fabrice Vavre; Laurence Vial
Journal:  Curr Biol       Date:  2018-05-31       Impact factor: 10.834

5.  Kinetics of African swine fever virus infection in Ornithodoros erraticus ticks.

Authors:  Afonso P Basto; Rebecca J Nix; Fernando Boinas; Susana Mendes; Maria J Silva; Clara Cartaxeiro; Raquel S Portugal; Alexandre Leitão; Linda K Dixon; Carlos Martins
Journal:  J Gen Virol       Date:  2006-07       Impact factor: 3.891

6.  First detection of African Swine Fever Virus in Ornithodoros porcinus in Madagascar and new insights into tick distribution and taxonomy.

Authors:  Julie Ravaomanana; Vincent Michaud; Ferran Jori; Abel Andriatsimahavandy; François Roger; Emmanuel Albina; Laurence Vial
Journal:  Parasit Vectors       Date:  2010-11-30       Impact factor: 3.876

7.  Comparative vector competence of the Afrotropical soft tick Ornithodoros moubata and Palearctic species, O. erraticus and O. verrucosus, for African swine fever virus strains circulating in Eurasia.

Authors:  Rémi Pereira de Oliveira; Evelyne Hutet; Frédéric Paboeuf; Maxime Duhayon; Fernando Boinas; Adalberto Perez de Leon; Serhii Filatov; Laurence Vial; Marie-Frédérique Le Potier
Journal:  PLoS One       Date:  2019-11-27       Impact factor: 3.240

8.  Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus' evolution.

Authors:  Jan H Forth; Leonie F Forth; Samantha Lycett; Lesley Bell-Sakyi; Günther M Keil; Sandra Blome; Sébastien Calvignac-Spencer; Antje Wissgott; Johannes Krause; Dirk Höper; Helge Kampen; Martin Beer
Journal:  BMC Biol       Date:  2020-10-08       Impact factor: 7.431

9.  Differential vector competence of Ornithodoros soft ticks for African swine fever virus: What if it involves more than just crossing organic barriers in ticks?

Authors:  Rémi Pereira De Oliveira; Evelyne Hutet; Renaud Lancelot; Frédéric Paboeuf; Maxime Duhayon; Fernando Boinas; Adalberto A Pérez de León; Serhii Filatov; Marie-Frédérique Le Potier; Laurence Vial
Journal:  Parasit Vectors       Date:  2020-12-09       Impact factor: 3.876
  9 in total

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