Literature DB >> 35575888

Laboratory Diagnosis and Quantification of African Swine Fever Virus Using Real-Time Polymerase Chain Reaction.

Christopher L Netherton1, Lynnette C Goatley1, John Flannery2, Martin Ashby2, Carrie Batten3.   

Abstract

Real-time polymerase chain reaction (PCR) for the detection of African swine fever virus (ASFV) is the tool of choice for the diagnostic laboratory and is a robust and easily scalable method for the researcher analyzing viral replication both in vitro and in vivo. In this chapter, we describe protocols for both quantitative real-time polymerase chain reactions (qPCR) and non-quantitative real-time polymerase chain reactions (real-time PCR) for the detection of African swine fever virus genome in a range of samples.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  African swine fever; Diagnosis; Real-time PCR; TaqMan®; qPCR

Mesh：

Year:  2022        PMID： 35575888     DOI： 10.1007/978-1-0716-2333-6_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  2 in total

Review 1.  False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy.

Authors:  A Borst; A T A Box; A C Fluit
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-03-10       Impact factor: 3.267

2.  Molecular diagnosis of African Swine Fever by a new real-time PCR using universal probe library.

Authors:  J Fernández-Pinero; C Gallardo; M Elizalde; A Robles; C Gómez; R Bishop; L Heath; E Couacy-Hymann; F O Fasina; V Pelayo; A Soler; M Arias
Journal:  Transbound Emerg Dis       Date:  2012-03-07       Impact factor: 5.005
  2 in total

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