Analysis of DWI in the classification of glioma pathology and its therapeutic application in clinical surgery: a case-control study.

Background: Glioma is a common primary craniocerebral malignant tumor, due to the lack of specificity of imaging examination and clinical manifestations, its diagnostic accuracy is relatively low, which may result in misdiagnosis and missed diagnosis. The apparent diffusion coefficient (ADC) in magnetic resonance diffusion weighted imaging (DWI) can reflect the histological characteristics of gliomas, which can be widely applied to classify gliomas and evaluate the extent of metastasis of glioma. The present study aimed to assess the clinical value of magnetic resonance DWI in the pathological grading of glioma and its therapeutic application in clinical surgery.
Methods: This article retrospectively analyzed the clinical data of 41 patients with glioma confirmed by surgical pathology results from January 1, 2019 to March 31, 2020 in the People's Hospital of Gaozhou. Among them, 16 patients had low-grade gliomas [World Health Organization (WHO) grade I-II] and 25 patients had high-grade gliomas (WHO grade III-IV). They were subjected to conventional T1WI and T2WI plain scans, along with DWI and enhanced scans before surgery. The ADC values of the glioma parenchyma, the peritumoral edema area, the surrounding white matter, and the contralateral normal white matter were measured. We selected some tumor tissues for pathological analysis as well, and conducted pathological grading according to WHO grading standards.
Results: We compared and evaluated the ADC values of the observed areas for low-grade gliomas and high-grade gliomas. The ADC values of low-grade gliomas in the tumor parenchyma, peritumoral edema, and white matter around the edema area were significantly lower than those of high-grade gliomas, and the differences were statistically significant (P<0.05). The difference in ADC values of normal white matter between the two groups of patients was not statistically significant (P=0.125). Conclusions: DWI has prognostic predictive value in the preoperative differential diagnosis and pathological classification of gliomas. This advanced technology can verify the extent of glioma infiltration in the surrounding brain tissue. It can help clinicians formulate a safer and more effective therapeutic strategy by providing accurate information on prognostic evaluation before the successful surgical intervention of gliomas. 2022 Translational Cancer Research. All rights reserved.

Introduction

Glioma is the most common primary brain malignant tumor, accounting for more than 35% of all intracranial tumors (1). Clinically, glioma originates from the canceration of brain glial cells, and displays varied clinical characteristics such as low cure rate, high mortality rate, and high recurrence rate. It poses a severe threat to the physical and mental health of patients. Some previous studies have shown that the survival rate of patients with glioma is closely related to the stage of the tumor, thus accurate diagnosis and surgical resection are of great significance to the prognosis of patients (2-4). With the development and progress of magnetic resonance imaging (MRI) technology in recent years, MRI examination for brain tumor evaluation has also received increasing attention. The apparent diffusion coefficient (ADC) in magnetic resonance diffusion weighted imaging (DWI) can reflect the histological characteristics of gliomas and is a measurement and imaging technique for the diffusion motion of water molecules in vivo. Using the ADC values from the biopsy site provides better representation of the actual tumor pathology (5). This technology can be widely applied to classify gliomas and evaluate the extent of metastasis of glioma (3), which has significant value towards the preoperative evaluation of tumor lesions. Because traditional DWI ignores the effect of capillary microcirculation, it cannot accurately display the actual movement of water molecules. The ADC parameters derived from DWI are affected by many physiological factors, including cell volume fraction, the transverse relaxation rate, and membrane permeability, and their ability to reflect complex problems is not enough. In this study, we analyzed and assessed the clinical value and application of DWI in the pathological grading of glioma towards preoperative evaluation of tumors. We present the following article in accordance with the STROBE reporting checklist (available at https://tcr.amegroups.com/article/view/10.21037/tcr-22-114/rc).

Methods

General information

This study retrospectively analyzed the clinical data of 41 patients with glioma confirmed by surgery and pathology from January 1, 2019 to March 31, 2020 in the People’s Hospital of Gaozhou. The patients included 16 cases of low-grade gliomas and 25 cases of high-grade gliomas. Among the low-grade glioma patients, 11 were males and 5 were females, aged 5 to 80 years, with an average age of 41.26±2.01 years. High-grade glioma patients included 20 males and 5 females, aged 8 to 80 years old, with an average age of 42.04±2.15 years old. The general data of low-grade and high-grade gliomas were not significantly different, but were comparable to each other (P>0.05). Due to the limited sample size, this study did not meet the World Health Organization (WHO) classification standards and analysis of metabolites and ratios in the peritumoral edema area, which may still require more supporting evidence with future clinical practice. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). This study was approved by the Medical Ethics Committee of the People’s Hospital of Gaozhou (No. GYLLPJ-2021061). Written informed consent was obtained from the patients and their families.

Inclusion and exclusion criteria

Inclusion criteria

The inclusion criteria were as follows: (I) cases confirmed as glioma by surgical pathological examination; (II) cases did not receive any relevant treatment before DWI examination; (III) patients voluntarily accepted DWI examination; (IV) all patients and their families provided informed consent for this study.

Exclusion criteria

The exclusion criteria were as follows: (I) the patient’s cooperation in examination was extremely poor, and mental illness was present; (II) examination had serious DWI artifacts; (III) the tumor had severe necrosis; (IV) glioma was accompanied by other craniocerebral diseases; (V) patients with missing clinical data.

Study methods

DWI examination

Using a 3 T scanner and a 32-channel head coil, all patients were subjected to conventional MRI and DWI scans (axial SE-T1WI, T2-FLAIR, FSE-T2WI, DWI scans), followed by coronary, axial, sagittal, and coronal T1WI enhanced scanning. Scanning parameter settings were as follows: T1WI: TE 10 ms, TR 350 ms; T2WI: TE 90 ms, TR 3,500 ms; TLAIR: T1 1,000 ms; layer thickness set to 5 mm, layer spacing set to 1.5 mm, matrix set to 256×256, FOV(field of view) set to 24 cm × 24 cm. DWI scanning was performed using a single-shot SE-EPI diffusion sequence, and the imaging parameters were: TE 90 ms, TR 8,000 s, and matrix 128×128, while FOV, layer spacing, and layer thickness were the same as the above scan parameters. In order to reduce the influence of different factors on the measurement of DWI signals and values, the diffusion coefficient sensitive gradient field was applied to the phase encoding, layer selection X axis, Y axis, and Z axis directions. The diffusion sensitivity coefficient (b value) was 1,000 s/mm2, and the scan was maintained for 30 s. After the examination was completed, the contrast agent DTPA was injected through the elbow vein for enhanced scanning.

DWI image data processing

We used the Syngo workstation to perform image post-processing analysis and the ADC software and b value to establish the ADC map. Two experienced imaging doctors performed the ADC measurements on the glioma parenchyma, the peritumoral edema area, its relatively normal white matter area, and the contralateral normal white matter area by carefully avoiding the necrotic area, cystic changes, sulci, and ventricles. For measurement, each area was measured 3 times, and the average of the 3 values was taken as the final result.

Observation indicators

High-grade glioma and low-grade glioma imaging performance was analyzed with parameters such as shape and boundaries of the lesion, uneven signals, signs of necrosis, and the degree of cystic changes, followed by evaluation and comparison of the measured ADC values in each area.

Pathological examination

We selected some tumor tissues of patients for pathological analysis: after the tumor specimens were treated by paraffin fixation, sectioning and HE staining, we observed cell morphology and histological structure under light microscope, and conducted pathological grading according to WHO grading standards.

Statistical methods

The data was analyzed using Statistical Product and Service Solutions (SPSS) 22.0 software. Measurement data was expressed as mean ± standard deviation () and compared using the t-test. P<0.05 was considered statistically significant.

Results

Imaging performance of low-grade glioma

A total of 16 patients with low-grade gliomas showed relatively regular morphology (). T2WI and T1WI showed high and low signals, respectively, with uniform signal expression and clear boundaries. There was no edema in the tumor, and the peritumoral edema range was narrower. DWI showed a slightly low signal or equal signal, and the enhanced scan of the tumor parenchyma had no or slight enhancement.

Figure 1

A male patient aged 33 years old showing anaplastic astrocytoma of the right temporal lobe (WHO II), the black arrow points to the tumor area. Axial T2WI (A) and T1WI (B) showing high and low signals, respectively. The signal expression was uniform, the boundary was clear, and there was no edema. DWI showed a slightly low signal (C). The enhanced scanning of the tumor parenchyma showed that it was slightly enhanced (D). WHO, World Health Organization; T2WI, T2 weighted image; T1WI, T1 weighted image; DWI, diffusion weighted imaging.

Imaging performance of high-grade glioma

A total of 25 patients with high-grade gliomas had irregular shapes, blurred boundaries, and uneven signals (). T2WI and T1WI showed slightly higher and lower signals, respectively, with clear signs of necrosis. The degree of cystic changes was apparent, and some had bleeding. DWI of the parenchyma showed a slightly higher signal. The area of cystic changes and necrosis showed a lower signal. There was obvious peritumoral edema, with finger-like edema in 20 cases. However, on enhanced scans, the tumor parenchyma was obviously strengthened, and there were 17 cases which showed obvious ring-shaped enhancement (the ring-shaped enhancement was thick with uneven thickness).

Figure 2

A male patient aged 43 years old, with anaplastic astrocytoma of the left frontal lobe (WHO IV), the black arrow points to the tumor area. T2WI (A) and T1WI (B) showed slightly higher and slightly lower signals, respectively. Necrotic cystic degeneration was also present, and some cases had bleeding. DWI of the parenchyma showed a slightly higher signal and cystosis with low signal necrosis. There was obvious peritumoral edema (C). After the enhanced scan, the tumor parenchyma was significantly strengthened (D). WHO, World Health Organization; T2WI, T2 weighted image; T1WI, T1 weighted image; DWI, diffusion weighted imaging.

Comparison of ADC values in each observation area of low-grade and high-grade gliomas

The ADC values of low-grade gliomas in the tumor parenchyma, peritumoral edema, and white matter surrounding the edema area were significantly lower than those of high-grade gliomas, which was considered statistically significant (P<0.05). There was no significant difference between the two groups of patients with normal white matter ADC values on the contralateral side (P>0.05, ).

Table 1

Comparison of ADC values in each observation area of low-grade and high-grade gliomas (, ×10−3 mm2/s)

Groups	Cases	Tumor parenchyma	Peritumoral edema	White matter surrounding edema area	Contralateral normal white matter
Low-grade glioma	16	1.66±0.45	1.53±0.36	0.85±0.22	0.73±0.35
High-grade glioma	25	1.60±0.32	1.18±0.39	0.74±0.54	0.74±0.30
T value	–	21.058	20.226	18.762	0.582
P value	–	0.000	0.000	0.000	0.125

ADC, the apparent diffusion coefficient.

Discussion

Gliomas are also called neuroepithelial tumors as they occur in the neuroectoderm. The World Health Organization (WHO) classifies neuroepithelial tumors into astrocytomas, oligodendrogliomas, choroid plexus tumors, ependymomas, medulloblastomas, mixed tumors, and other neuroepithelial tumors. A major biological characteristic of gliomas is infiltrative growth, which cannot be easily distinguished from adjacent brain tissue. Especially for high-grade gliomas, complete resection is more difficult, and the postoperative recurrence rate is usually higher. The clinical treatment for glioma is surgical resection combined with radiotherapy and chemotherapy, of which surgical intervention is the preferred treatment for glioma. The principle of surgical intervention is to remove the tumor mass to the greatest possible extent under strict supervision with precautionary measures to ensure the complete restoration of normal functions. Therefore, accurate evaluation of the tumor grade before surgery is particularly critical for the implementation of accurate therapeutic strategies and the evaluation of tumor prognosis (4). The clinical diagnosis of gliomas mainly depends on conventional MRI examinations, which are mainly assessed through the clear identification of tumor signals, morphology, edema around the tumor, and the enhanced tumor augmentation, to initially differentiate and diagnose low-grade or high-grade gliomas. The edematous area which develops around the glioma is mainly angiogenic edema, and some edematous area are usually caused by tumor cell infiltration, but it is more challenging to differentiate them through conventional MRI examination. There are certain limitations in the accurate evaluation of infiltration which develops around the tumor (6). At present, DWI is the only non-invasive method for measuring the motion of the water molecules in living tissue. By measuring the ADC value, it can clarify the microscopic diffusion movement of water molecules inside and outside living tissues, and then understand the quantitative movement of water molecules (7). Choi et al. (8) reported that there is a close relationship between the ADC value and cell structure. The movement of water molecules will be hindered by the tumor cells, limiting their spread, and DWI examination can show these abnormal signals. The greater the density of tumor stroma, the smaller the extracellular space, and the free diffusion of water molecules is restricted, which may reduce the ADC value correspondingly. In contrast, when tumor density is lower, the diffusion capacity of water molecules increases, and the ADC value is enhanced.

DWI can quantitatively detect the permeability of immature tumor microvessels, mainly through the use of phase refocusing, to objectively and quantitatively evaluate the degree of microscopic diffusion of water molecules in tissues. DWI can efficiently evaluate the cell density, microvessel density, white matter cellulose integrity, and morphological changes of glioma tissue, and then effectively analyze the biological behavior of patients with gliomas (9). With the rapid advancement of technology, various MRI techniques have been applied to preoperative tumor grading of gliomas, especially in differentiating high-grade from low-grade gliomas. When the diffusion sensitivity coefficient (b value) of DWI imaging reaches 5,000 s/mm2 during the examination, the specific performance of the structural organization can be verified clearly. When the b value increases, the sensitivity for detecting water molecule diffusion can be improved, thereby reducing the T2 transmission effect. In recent years, more and more b values have been used in DWI examinations in clinical practice (10). The results of this study showed that the ADC value of the parenchyma of high-grade glioma was lower than that of low-grade glioma, which is consistent with the results of Togao et al. (11). Liu et al. (12) showed that the ADC value of the tumor parenchyma area had a sensitivity and specificity of 86.36% and 90.0%, respectively, for the identification of low and high-grade gliomas, indicating that the ADC value can more efficiently differentiate and grade gliomas. High-grade gliomas are more likely to infiltrate peritumoral cells. The smaller the distance from the tumor, the more cell infiltration will occur in the edema area. However, low-grade gliomas are less infiltrating into the surrounding cells (13).

The results of our study showed that the ADC values in the peritumoral edema and peripheral white matter areas of high-grade gliomas were lower than those of low-grade gliomas. In contrast, the ADC values of contralateral normal brain tissue were not significantly different. Moreover, it is apparent that the peritumoral edema ADC value can reveal the infiltration of high-grade glioma, and low-grade glioma has less obvious peritumoral infiltration. Therefore, DWI can also help clinicians judge the degree of peritumoral invasion of gliomas and effectively plan the surgical resection of glioma in a smooth and effective way. Existing studies have found that the DWI method alone is not specific and sensitive in the diagnosis of gliomas (14). Because traditional DWI ignores the effect of capillary microcirculation, it cannot accurately display the actual movement of water molecules. The ADC parameters derived from DWI are affected by many physiological factors, including cell volume fraction, the transverse relaxation rate, and membrane permeability, and their ability to reflect complex problems is relatively low. Moreover, due to the limited sample size, this study did not meet the WHO classification standards and analysis of metabolites and ratios in the peritumoral edema area, which may still require more supporting evidence with future clinical practice.

Federau et al. (15) have proposed an MRI method called intravoxel incoherent motion imaging, which can efficiently extract information on quantitative microvascular perfusion through multi-b-value DWI. The main parameters are pseudodiffusion coefficient (D*), perfusion fraction (f), the product of f and D* (f×D*), true diffusion coefficient (D), and ADC. Shen et al. found that the ADC and D values of high-grade gliomas were significantly lower than those of low-grade gliomas, and the f×D* values were significantly different in grades Ⅱ to Ⅳ gliomas (16). Recently, Togao et al. have reported that the f value of high-grade glioma is significantly higher than that of low-grade glioma (11). It can be seen that the 5 parameters derived from intravoxel incoherent motion imaging are promising markers for preoperative glioma grading, and the ADC value is only one of them. As our study reported here is based on single parameter analysis, more comprehensive studies designed with all 4 parameters can provide more conclusive evidence.

In summary, DWI has certain prognostic predictive value in the differential diagnosis of gliomas prior to surgical intervention, and can help clinicians plan a therapeutic strategy in a timely manner towards effective management of gliomas.