20 (OH) ecdysone-induced transition from intermolt to premolt protein biosynthesis patterns in the hypodermis of the crayfish, Astacus leptodactylus, in vitro.

20 (OH) Ecdysone-induced alteration of protein biosynthesis and secretion by the hypodermis of the crayfish Astacus leptodactylus was studied using isolated tissues in vitro. Characteristic quantitative and qualitative changes of late intermolt tissues are governed by the hormone in a dose- and time-dependent manner. The greatest response to the hormone was observed in tissues which were exposed for 48 hr to 10(-8) M 20 (OH) ecdysone, followed by 1 day of hormone withdrawal. The observed differences correspond to the reprogramming in protein biosynthesis after transition from intermolt to the premolt stage.