Degradation and detoxification of reactive yellow dyes by Scedosporium apiospermum: a mycoremedial approach.

Textile industrial effluents have long enunciated the essentiality of ascertaining an efficient wastewater treatment for the removal of azo dyes given their potential disturbances on the ecosystem. Our study investigated the efficiency of the strain SKF2 among 14 other isolates, molecularly identified to be Scedosporium apiospermum, isolated by our research group from the textile effluent sludge in the degradation of two azo dyes, Reactive Yellow 145 and Remazol Yellow RR. Kinetic profiling of the degradation process revealed the decolourisation efficiency to be 94.8 and 86.9% for RY 145 and RYRR, respectively, during the declining growth phase. Laccase and polyphenol oxidase (RY 145-2.37 and RYRR-2.30 U/mL; RY 145-3.26 and RYRR-2.89 U/mL, respectively) were found to influence the biodegradation process in both the dyes than the other examined fungal degradative enzymes. The metabolic pathway predicted with the aid of GC-MS analysis identified the degraded metabolites to be smaller molecular weight non-toxic products. Assessment of toxicity via brine shrimp lethality assay (RY 145-23.3% and RYRR-16.7%, respectively) and seed germination assay (RY 145-96.7% and RYRR-83.3%) further solidified the detoxified status of both the dyes after biodegradation. The experimental data thus substantiated the expediency of S. apiospermum SKF2 in the degradation of textile azo dyes and its further employment in the bioremediation of textile wastewaters for agricultural applications and ecological recycling.