Hunter A Miller1, Shesh N Rai1,2,3, Xinmin Yin4, Xiang Zhang4, Jason A Chesney1,3,5,6, Victor H van Berkel3,7, Hermann B Frieboes8,9,10,11. 1. Department of Pharmacology and Toxicology, University of Louisville, Louisville, USA. 2. Department of Bioinformatics and Biostatistics, University of Louisville, Louisville, USA. 3. James Graham Brown Cancer Center, University of Louisville, Louisville, USA. 4. Department of Chemistry, University of Louisville, Louisville, USA. 5. Division of Medical Oncology and Hematology, Department of Medicine, University of Louisville, Louisville, USA. 6. Department of Biochemistry and Molecular Genetics, University of Louisville, Louisville, USA. 7. Department of Cardiovascular and Thoracic Surgery, University of Louisville, Louisville, USA. 8. Department of Pharmacology and Toxicology, University of Louisville, Louisville, USA. hbfrie01@louisville.edu. 9. James Graham Brown Cancer Center, University of Louisville, Louisville, USA. hbfrie01@louisville.edu. 10. Department of Bioengineering, University of Louisville, Lutz Hall 419, Louisville, KY, 40292, USA. hbfrie01@louisville.edu. 11. Center for Predictive Medicine, University of Louisville, Louisville, USA. hbfrie01@louisville.edu.
Abstract
INTRODUCTION: Metabolomics has emerged as a powerful method to provide insight into cancer progression, including separating patients into low- and high-risk groups for overall (OS) and progression-free survival (PFS). However, survival prediction based mainly on metabolites obtained from biofluids remains elusive. OBJECTIVES: This proof-of-concept study evaluates metabolites as biomarkers obtained directly from tumor core biopsies along with covariates age, sex, pathological stage at diagnosis (I/II vs. III/VI), histological subtype, and treatment vs. no treatment to risk stratify lung cancer patients in terms of OS and PFS. METHODS: Tumor core biopsy samples obtained during routine lung cancer patient care at the University of Louisville Hospital and Norton Hospital were evaluated with high-resolution 2DLC-MS/MS, and the data were analyzed by Kaplan-Meier survival analysis and Cox proportional hazards regression. A linear equation was developed to stratify patients into low and high risk groups based on log-transformed intensities of key metabolites. Sparse partial least squares discriminant analysis (SPLS-DA) was performed to predict OS and PFS events. RESULTS: Univariable Cox proportional hazards regression model coefficients divided by the standard errors were used as weight coefficients multiplied by log-transformed metabolite intensity, then summed to generate a risk score for each patient. Risk scores based on 10 metabolites for OS and 5 metabolites for PFS were significant predictors of survival. Risk scores were validated with SPLS-DA classification model (AUROC 0.868 for OS and AUROC 0.755 for PFS, when combined with covariates). CONCLUSION: Metabolomic analysis of lung tumor core biopsies has the potential to differentiate patients into low- and high-risk groups based on OS and PFS events and probability.
INTRODUCTION: Metabolomics has emerged as a powerful method to provide insight into cancer progression, including separating patients into low- and high-risk groups for overall (OS) and progression-free survival (PFS). However, survival prediction based mainly on metabolites obtained from biofluids remains elusive. OBJECTIVES: This proof-of-concept study evaluates metabolites as biomarkers obtained directly from tumor core biopsies along with covariates age, sex, pathological stage at diagnosis (I/II vs. III/VI), histological subtype, and treatment vs. no treatment to risk stratify lung cancer patients in terms of OS and PFS. METHODS: Tumor core biopsy samples obtained during routine lung cancer patient care at the University of Louisville Hospital and Norton Hospital were evaluated with high-resolution 2DLC-MS/MS, and the data were analyzed by Kaplan-Meier survival analysis and Cox proportional hazards regression. A linear equation was developed to stratify patients into low and high risk groups based on log-transformed intensities of key metabolites. Sparse partial least squares discriminant analysis (SPLS-DA) was performed to predict OS and PFS events. RESULTS: Univariable Cox proportional hazards regression model coefficients divided by the standard errors were used as weight coefficients multiplied by log-transformed metabolite intensity, then summed to generate a risk score for each patient. Risk scores based on 10 metabolites for OS and 5 metabolites for PFS were significant predictors of survival. Risk scores were validated with SPLS-DA classification model (AUROC 0.868 for OS and AUROC 0.755 for PFS, when combined with covariates). CONCLUSION: Metabolomic analysis of lung tumor core biopsies has the potential to differentiate patients into low- and high-risk groups based on OS and PFS events and probability.
Authors: Teresa W M Fan; Andrew N Lane; Richard M Higashi; Mohamed A Farag; Hong Gao; Michael Bousamra; Donald M Miller Journal: Mol Cancer Date: 2009-06-26 Impact factor: 27.401
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Authors: Laurent Dercle; Matthew Fronheiser; Lin Lu; Shuyan Du; Wendy Hayes; David K Leung; Amit Roy; Julia Wilkerson; Pingzhen Guo; Antonio T Fojo; Lawrence H Schwartz; Binsheng Zhao Journal: Clin Cancer Res Date: 2020-03-20 Impact factor: 13.801