Acute Toxicity Bioassay and Determination of LC50 of Cadmium Chloride in Trichogaster (Colisa) fasciata.

Cadmium chloride is a metal salt used in industries in a large scale. Trichogaster (Colisa) fasciata (common name banded gourami) is an air-breathing fresh water fish with both nutritional and ornamental values. In this present study, as per EPA guidelines, acute toxicity bioassay was performed in a 96-h static test method in four replicates, each replicate having one control and five concentrations (25, 50, 75, 100 and 125 mg/L, respectively) of the metal salt. A total of 192 fish was used in the study in four series with each replicate containing 8 fish per concentration. The water temperature was maintained at 20-22℃ during the study. The data observed were analysed statistically on the basis of Finney's Probit Analysis method using SPSS software. The estimated 96-h LC50 value of cadmium chloride for the fish was 49.5 mg/L.