Inhibition effect of high hydrostatic pressure combined with epigallocatechin gallate treatments on pectin methylesterase in orange juice and model system.

High hydrostatic pressure (HHP) is currently the most successful non-thermal processing technology for commercial applications, but with a drawback that it is difficult to effectively inactivate the pectin methylesterase (PME), which is critical to the stability of orange juice. In this study, the PME inhibition and mechanism by HHP (600 MPa/10 min) combined with epigallocatechin gallate (HHP-EGCG) treatment were investigated. Firstly, the HHP-EGCG treatment showed enhancement effect on PME inhibition in orange juice, and the samples maintained higher content of water soluble pectin and exhibited higher suspension stability than the HHP treated samples during 13 days of refrigerated storage. Secondly, after HHP-EGCG treatment, further synergistic effect was observed in the phosphate buffer system, and the greatest secondary structure transformation and fluorescence quenching of PME occurred. Finally, molecule docking suggested that EGCG could interact with the active sites of PME, and transmission electron microscope results revealed further aggregation of PME under HHP-EGCG treatment.