Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Radical SAM-dependent formation of a nitrogenase cofactor core on NifB.

Literature DB >> 35550498

Radical SAM-dependent formation of a nitrogenase cofactor core on NifB.

Yiling A Liu¹, Robert Quechol¹, Joseph B Solomon², Chi Chung Lee¹, Markus W Ribbe³, Yilin Hu⁴, Britt Hedman⁵, Keith O Hodgson⁶.

Abstract

Nitrogenase is a versatile metalloenzyme that reduces N2, CO and CO2 at its cofactor site. Designated the M-cluster, this complex cofactor has a composition of [(R-homocitrate)MoFe7S9C], and it is assembled through the generation of a unique [Fe8S9C] core prior to the insertion of Mo and homocitrate. NifB is a radical S-adenosyl-L-methionine (SAM) enzyme that is essential for nitrogenase cofactor assembly. This review focuses on the recent work that sheds light on the role of NifB in the formation of the [Fe8S9C] core of the nitrogenase cofactor, highlighting the structure, function and mechanism of this unique radical SAM methyltransferase.

Entities: Chemical

Keywords: Biosynthesis; FeS cluster; M-cluster; NifB; Nitrogenase; Radical SAM enzyme

Mesh：

Substances：

Year: 2022 PMID： 35550498 PMCID： PMC9526504 DOI： 10.1016/j.jinorgbio.2022.111837

Source DB: PubMed Journal: J Inorg Biochem ISSN： 0162-0134 Impact factor: 4.336

Keyword Cloud
References

78 in total

Review 1. The Radical SAM Superfamily.

Authors: Perry A Frey; Adrian D Hegeman; Frank J Ruzicka
Journal: Crit Rev Biochem Mol Biol Date: 2008 Jan-Feb Impact factor: 8.250

2. Vanadium nitrogenase reduces CO.

Authors: Chi Chung Lee; Yilin Hu; Markus W Ribbe
Journal: Science Date: 2010-08-06 Impact factor: 47.728

3. Heterologous Expression and Engineering of the Nitrogenase Cofactor Biosynthesis Scaffold NifEN.

Authors: Joseph B Solomon; Chi Chung Lee; Andrew J Jasniewski; Mahtab F Rasekh; Markus W Ribbe; Yilin Hu
Journal: Angew Chem Int Ed Engl Date: 2020-03-03 Impact factor: 15.336

Review 4. Structural insights into radical generation by the radical SAM superfamily.

Authors: Jessica L Vey; Catherine L Drennan
Journal: Chem Rev Date: 2011-03-03 Impact factor: 60.622

5. Structural basis for glycyl radical formation by pyruvate formate-lyase activating enzyme.

Authors: Jessica L Vey; Jian Yang; Meng Li; William E Broderick; Joan B Broderick; Catherine L Drennan
Journal: Proc Natl Acad Sci U S A Date: 2008-10-13 Impact factor: 11.205

6. Crystal structure of the S-adenosylmethionine-dependent enzyme MoaA and its implications for molybdenum cofactor deficiency in humans.

Authors: Petra Hänzelmann; Hermann Schindelin
Journal: Proc Natl Acad Sci U S A Date: 2004-08-18 Impact factor: 11.205

Radical SAM-dependent formation of a nitrogenase cofactor core on NifB.

Review 1. The Radical SAM Superfamily.

2. Vanadium nitrogenase reduces CO.

3. Heterologous Expression and Engineering of the Nitrogenase Cofactor Biosynthesis Scaffold NifEN.

Review 4. Structural insights into radical generation by the radical SAM superfamily.

5. Structural basis for glycyl radical formation by pyruvate formate-lyase activating enzyme.

6. Crystal structure of the S-adenosylmethionine-dependent enzyme MoaA and its implications for molybdenum cofactor deficiency in humans.

Review 7. Structural Conversions of Synthetic and Protein-Bound Iron-Sulfur Clusters.

8. Comment on "Structural evidence for a dynamic metallocofactor during N₂ reduction by Mo-nitrogenase".

9. Identity and function of an essential nitrogen ligand of the nitrogenase cofactor biosynthesis protein NifB.

10. An unexpected P-cluster like intermediate en route to the nitrogenase FeMo-co.