Literature DB >> 3552815

High-performance liquid chromatography (HPLC): a rapid, flexible and sensitive method for separating islet proinsulin and insulin.

P A Halban, C J Rhodes, S E Shoelson.   

Abstract

Evaluating islet function in vitro involves studying both insulin biosynthesis and release. For the former, it is necessary to resolve insulin from its precursor, proinsulin. This has been achieved in the past by various procedures, each of which suffers from major drawbacks in terms of resolution and the time involved. We show here that reversed phase high-performance liquid chromatography (HPLC) outperforms previous methods for separating proinsulin from insulin in islet extracts without any prepurification or concentration steps. This HPLC method is rapid (90 min for a complete cycle, including washing the column) and reproducible, while allowing for unambiguous separation and quantification of proinsulin and insulin.

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Year:  1986        PMID： 3552815     DOI： 10.1007/BF00870146

Source DB:  PubMed          Journal:  Diabetologia        ISSN： 0012-186X            Impact factor:   10.122


  8 in total

1.  Insulin biosynthesis in the rat: demonstration of two proinsulins.

Authors:  J L Clark; D F Steiner
Journal:  Proc Natl Acad Sci U S A       Date:  1969-01       Impact factor: 11.205

2.  Reversed-phase high-performance liquid chromatography of insulins from different species.

Authors:  J Rivier; R McClintock
Journal:  J Chromatogr       Date:  1983-09-23

3.  Pancreatic content of insulins I and II in laboratory rodents. Analysis by immunoelectrophoresis.

Authors:  K Kakita; K O'Connell; M A Permutt
Journal:  Diabetes       Date:  1982-10       Impact factor: 9.461

4.  Perturbation of hormone storage and release induced by cyproheptadine in rat pancreatic islets in vitro.

Authors:  P A Halban; C B Wollheim; B Blondel; E Niesor; A E Renold
Journal:  Endocrinology       Date:  1979-04       Impact factor: 4.736

5.  Biochemical and clinical implications of proinsulin conversion intermediates.

Authors:  B D Given; R M Cohen; S E Shoelson; B H Frank; A H Rubenstein; H S Tager
Journal:  J Clin Invest       Date:  1985-10       Impact factor: 14.808

6.  Inhibition of proinsulin to insulin conversion in rat islets using arginine and lysine analogs. Lack of effect on rate of release of modified products.

Authors:  P A Halban
Journal:  J Biol Chem       Date:  1982-11-25       Impact factor: 5.157

7.  Three mutant insulins in man.

Authors:  S Shoelson; M Haneda; P Blix; A Nanjo; T Sanke; K Inouye; D Steiner; A Rubenstein; H Tager
Journal:  Nature       Date:  1983-04-07       Impact factor: 49.962

8.  Comparison of prohormone-processing activities in islet microsomes and secretory granules: evidence for distinct converting enzymes for separate islet prosomatostatins.

Authors:  B D Noe; G Debo; J Spiess
Journal:  J Cell Biol       Date:  1984-08       Impact factor: 10.539
  8 in total
  10 in total

1.  Changes in metabolism and hormone trafficking during exposure of endocrine cells to elevated ammonium.

Authors:  J J Dyken-Young; A Sambanis
Journal:  Cytotechnology       Date:  1995-02       Impact factor: 2.058

2.  Partial diversion of a mutant proinsulin (B10 aspartic acid) from the regulated to the constitutive secretory pathway in transfected AtT-20 cells.

Authors:  D J Gross; P A Halban; C R Kahn; G C Weir; L Villa-Komaroff
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

3.  Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans.

Authors:  G A Spinas; B S Hansen; S Linde; W Kastern; J Mølvig; T Mandrup-Poulsen; C A Dinarello; J H Nielsen; J Nerup
Journal:  Diabetologia       Date:  1987-07       Impact factor: 10.122

4.  High-performance liquid chromatography analysis of circulating insulins distinguishes between endogenous insulin production (a potential pitfall with streptozotocin diabetic rats) and islet xenograft function.

Authors:  D Chicheportiche; S Darquy; J Lepeintre; F Capron; P A Halban; G Reach
Journal:  Diabetologia       Date:  1990-08       Impact factor: 10.122

5.  A sensitive and reliable method for assaying true human insulin without interaction with human proinsulin-like molecules.

Authors:  L D Monti; E P Sandoli; V C Phan; P M Piatti; S Costa; A Secchi; G Pozza
Journal:  Acta Diabetol       Date:  1995-03       Impact factor: 4.280

6.  Effects of aging on insulin synthesis and secretion. Differential effects on preproinsulin messenger RNA levels, proinsulin biosynthesis, and secretion of newly made and preformed insulin in the rat.

Authors:  S Y Wang; P A Halban; J W Rowe
Journal:  J Clin Invest       Date:  1988-01       Impact factor: 14.808

7.  Differential rates of conversion of rat proinsulins I and II. Evidence for slow cleavage at the B-chain/C-peptide junction of proinsulin II.

Authors:  S V Sizonenko; P A Halban
Journal:  Biochem J       Date:  1991-09-15       Impact factor: 3.857

8.  The intracellular handling of insulin-related peptides in isolated pancreatic islets. Evidence for differential rates of degradation of insulin and C-peptide.

Authors:  C J Rhodes; P A Halban
Journal:  Biochem J       Date:  1988-04-01       Impact factor: 3.857

9.  Newly synthesized proinsulin/insulin and stored insulin are released from pancreatic B cells predominantly via a regulated, rather than a constitutive, pathway.

Authors:  C J Rhodes; P A Halban
Journal:  J Cell Biol       Date:  1987-07       Impact factor: 10.539

10.  pH-independent and -dependent cleavage of proinsulin in the same secretory vesicle.

Authors:  L Orci; P Halban; A Perrelet; M Amherdt; M Ravazzola; R G Anderson
Journal:  J Cell Biol       Date:  1994-09       Impact factor: 10.539
  10 in total

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