Prostate cancer is a slowly developing disease with a high mortality rate in men, especially in Western countries (1). Castrate-resistant prostate cancer (CRPC) is resistant to androgen ablation and cancer metastases are often observed in patients with CRPC (2). Cancer metastasis is a complex mechanism and cascade of events that allows tumor cells to travel to other organs. Epithelial-mesenchymal transition (EMT) is as an important event in the initial steps of cancer cell metastasis (3). The loss of epithelial cell characteristics leads to the transformation of epithelial cells to mesenchymal cells with a stem cell-like phenotype. Notably, EMT can result in increasing resistance to apoptosis and chemotherapy (4,5). Previous studies have reported that lipopolysaccharide (LPS), a component of gram-negative bacteria, can trigger EMT, which induces the migration and invasion of cancer cells (6–8).Autophagy regulates cell damage and degradation and processes the recycling of cell constituents. It is an adaptive process and a form of cell death that occurs in response to stress, including elevated levels of reactive oxygen species (ROS) and anticancer agents (9,10). Autophagy may therefore serve a pivotal role during chemotherapy. Phytochemicals or chemotherapeutic agents can overcome drug resistance and induce apoptosis in cancer cells (11–13).Ferroptosis is a form of cell death, which has characteristics that are different from apoptosis and autophagy. The accumulation of intracellular iron and ROS, and the depletion of glutathione (GSH) are characteristic of ferroptosis (14). Ferroptosis inducers can inhibit cancer cell proliferation and may be a novel target for potential cancer therapeutics (15,16).Dietary natural products contain numerous bioactive phytochemicals with a wide spectrum of pharmacological activities. Ginger (Zingiber officinale) is commonly used as a spice and a traditional medicine (17). One component of ginger extract, 6-Gingerol, has anti-inflammatory, anticancer and antioxidant effects (18–21). In addition, 6-Gingerol has been reported to exhibit synergistic effects on PC3 cells by inducing apoptosis (22) and to inhibit testosterone-induced proliferation of LNCaP cells (23). However, to the best of our knowledge, whether 6-Gingerol also inhibits EMT, and induces autophagy or ferroptosis in prostate cancer cells is unknown.The present study aimed to determine the pharmacological effects of 6-Gingerol against LPS-induced migration and invasion, and the potential of 6-Gingerol to inhibit LPS-induced EMT in prostate cancer cells. It can therefore be hypothesized that 6-Gingerol may be used as an effective chemotherapeutic agent to treat prostate cancer.
Materials and methods
Chemicals and reagents
6-Gingerol (95–99% purity, determined by high-performance liquid chromatography) was purchased from Chengdu Biopurify Phytochemicals, Ltd. PI3K inhibitor (LY294002) and MTT reagent were purchased from Beyotime Institute of Biotechnology. LPS (from Escherichia coli 026:B6) and β-actin primary antibodies (cat. no. A5441) were obtained from MilliporeSigma. Ferrostatin-1 was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Primary antibodies against Beclin-1 (cat. no. AB3219), LC3B (cat. no. CY5992), nuclear factor erythroid 2-related factor 2 (NRF2; cat. no. CY1851) and GSH peroxidase (GPX) 4 (cat. no. CY6959) were purchased from Shanghai Abways Biotechnology Co., Ltd. Primary antibodies against E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), Vimentin (cat. no. 5741) and zonula occludens-1 (ZO-1; cat. no. 8193) were purchased from Cell Signaling Technology, Inc. Anti-rabbit IgG horseradish peroxidase HRP-linked antibody (cat. no. 7074) and anti-mouse IgG HRP-linked antibody (cat. no. 7076) were purchased from Cell Signaling Technology, Inc.
Cell culture
The human prostate cancer LNCaP, DU145 and PC3 cell lines were purchased from Shanghai Fuheng Biotechnology Co., Ltd. Cells were cultured at 37°C in a humidified atmosphere with 5% CO2. LNCaP cells were grown in RPMI-1640 medium, DU145 and PC3 cells were grown in DMEM/Ham's F12 Kaighn's (K) medium (both LONSERA ShangHai ShuangRu Biotech Co., Ltd). The media were supplemented with 10% fetal bovine serum (FBS; LONSERA ShangHai ShuangRu Biotech Co., Ltd.), 100 U/ml penicillin and 100 µg/ml streptomycin (Beyotime Institute of Biotechnology). In each experiment, the control group was untreated cells.
Cell viability assay
LNCaP, DU145 and PC3 cells were seeded at a density of 1×104 cells/well in 96-well plates. When cells reached 80% confluency, cells were treated with 6-Gingerol (1–500 µM), with or without LPS (1 µg/ml), ferrostatin-1 (5 µM) and LY294002 (10 µM), at 37°C for 24, 48 or 72 h. After incubation, cell viability was determined using an MTT assay. The medium was replaced with fresh medium, 10 µl MTT (5 mg/ml) was added to each well contain 100 µl fresh medium and cells were incubated at 37°C for 4 h. The supernatant was subsequently discarded and 100 µl DMSO was used to dissolve the MTT-formazan crystals. Absorbance was then quantified using a microplate reader at a wavelength of 570 nm.
Colony formation
LNCaP, DU145 and PC3 cells were seeded into a 6-well plate at a density of 5×102 cells/well. Cells were incubated at 37°C for 4 h and were subsequently treated with different concentrations (1, 10, 100 and 500 µM) of 6-Gingerol. After incubation at 37°C for 7 days without changing the medium, 4% formaldehyde was applied for fixing cells for 20 min at room temperature and stained with 0.2% crystal violet for 20 min at room temperature. Colonies were defined as groups of >50 cells and manually counted under an inverted light microscope (Nikon TI-DH).
Wound healing assay
DU145 and PC3 cells at a density of 1×106 cells/well were cultured on a 6-well plate with medium containing 10% FBS. After reaching 100% confluency, the medium was replaced with serum-free medium. A scratch was created on the cell monolayers using a sterile 200-µl pipette tip and cells were then treated with 6-Gingerol (10 µM), with or without LPS (1 µg/ml) at 37°C for 24 or 48 h. The images were observed and captured by image device (NIS Elements version 4.30, Nikon) and inverted light microscope (Nikon TI-DH). Wound healing was semi-quantified using ImageJ 1.52a software (National Institutes of Health). The wound area was calculated as the follows: (Initial wound width-final wound width)/initial wound width ×100 (%).
Cell adhesion assay
Fibronectin (Beijing Solarbio Science & Technology Co., Ltd.) was dissolved in PBS and used for coating. Then, 0.1 ml of fibronectin (5 µg/ml) was added per well in a 96-well plate at 4°C overnight. After incubation, the wells were washed with PBS twice and incubated with serum-free medium at 37°C for 30 min. LNCaP, DU145 and PC3 cells (1×104) were added to each well in fresh medium containing 6-Gingerol (100 and 500 µM), with or without LPS (1 µg/ml) incubated at 37°C at 1 and 2 h for adhesion. After incubation, the adhered cells were gently washed twice with PBS and measured using MTT assay, as aforementioned.
Migration and invasion assays
The migratory and invasive abilities of DU145 and PC3 cells were determined using 8-µm Transwell filter membranes (Costar; Corning, Inc.). For the migration assay, 1×104 cells were seeded into the upper chamber with DMEM/Ham's F12K serum-free medium containing 6-Gingerol (10 µM), whereas the bottom chamber was loaded with DMEM/Ham's F12K medium containing 10% FBS with or without LPS (1 µg/ml) as a chemoattractant. After incubation at 37°C for 48 h, cells in the upper chamber were gently scraped off and the migrating cells that had accumulated in the bottom chamber were fixed with 4% formaldehyde for 20 min at room temperature and stained with 0.2% crystal violet for 20 min at room temperature. The migrated cells on the bottom surface of the membrane were captured (NIS Elements version 4.30, Nikon) and counted manually under an inverted light microscope (Nikon TI-DH). For the invasion assay, each Transwell plate was coated with Matrigel (1 mg/ml, Corning, Inc.) with serum free medium at 37°C for 1 h. The subsequent procedure was the same as that of migration assay.
Western blotting
To examine the mechanism of underling the anti-cancer effects of 6-Gingerol on prostate cancer cells, LNCaP, DU145 and PC3 cells were treated with 6-Gingerol (1–100 µM), with or without LPS (1 µg/ml) and ferrostatin-1 (5 µM) at 37°C for 24 or 48 h. After incubation, total protein was extracted by M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, Inc.; cat. no. 78505). The concentration of protein was determined by Pierce Coomassie (Bradford) Protein Assay Kit (Thermo Scientific, cat. no. 23200) and was separated by 7.5, 10.0 or 12.0% SDS-PAGE (20 µg total protein/lane). Separated proteins were subsequently transferred onto a PVDF membrane. The membranes were blocked with 5% non-fat dried milk 1X TBST buffer (20 mM Tris, 150 mM NaCl, 0.1% Tween 20) at room temperature for 1 h. Membranes were incubated at 4°C overnight with the following primary antibodies: Beclin-1 (1:1,000), LC3B (1:1,000), NRF2 (1:1,000), GPX4 (1:1,000), E-cadherin (1:1,000), N-cadherin (1:1,000), Vimentin (1:1,000), β-actin (1:8,000) and ZO-1 (1:1,000). Subsequently, membranes were incubated for 1 h at room temperature with the secondary antibodies, anti-rabbit IgG HRP-linked antibody (1:1,000) and anti-mouse IgG HRP-linked antibody (1:1,000). Protein bands were subsequently visualized using an enhanced chemiluminescent kit to determine protein expression (Shanghai Epizyme Biomedical Technology Co., Ltd). The bands were detected using a ChemiScope 3300 Mini (Clinx Science Instruments Co., Ltd.). β-actin was used as the internal control for Western Blots. The densitometry of protein expression was determined using ImageJ 1.52a software (National Institutes of Health, USA).
Determination of intracellular ROS and GSH
Intracellular ROS levels were determined using reactive oxygen species assay kit (Biosharp; cat. no. BL714A). according to the manufacturer's protocol. Briefly, the LNCaP, DU145 and PC3 cells were cultured in 6-well plates at density of 1×105 cells. Cells were treated with 6-Gingerol (100 µM) with or without ferrostatin-1 (5 µM) at 37°C for 24 h. After the incubation, the cells were collected, stained with H2DCFH-DA (10 µM) at 37°C for 30 min in the dark and then washed twice with serum free medium. For each experiment, the fluorescence intensity of ROS was quantified using flow cytometry (NovoCyte Flow Cytometer; Agilent Technologies, Inc.). Data were analyzed using NovoExpress 1.2.5 software (2016 ACEA Biosciences, Inc.). GSH levels were determined using a Glutathione Assay Kit (Nanjing Jiancheng Bioengineering Institute; cat. no. A006-2-1). LNCaP, DU145 and PC3 cells at the density of 1×104 were seeded into a 24-well plate and incubated overnight at 37°C. Cells were treated with 6-Gingerol (10, 100 µM) with or without ferrostatin-1 (5 µM) at 37°C for 24 h. Cells were then collected and homogenized. After centrifugation at 14,000 g for 10 min at 4°C, the supernatant was collected and GSH levels quantified according to the manufacturer's instructions. The absorbance was measured using a microplate reader at the wavelength of 405 nm. The content of GSH levels were determined by the standard curve.
Statistical analysis
The experiments were performed at three times independently and the data analysis were done by Excel (Microsoft 365MSO, 16.0.14931.20118). Statistical comparisons among more than two groups were performed using one-way ANOVA followed by Tukey's post hoc test. All data are presented as the mean ± SEM. P<0.05 was considered to indicate a statistically significant difference.
Results
6-Gingerol suppresses cell viability and colony formation in prostate cancer cells
LNCaP, PC3 and DU145 cells were treated with 6-Gingerol (1–500 µM) for 24, 48 or 72 h. The viability of LNCaP, PC3 and DU145 cells was inhibited by the different 6-Gingerol (1–500 µM) treatments. The cell survival rate with 6-Gingerol (500 µM) at 72 h was 46.08±4.29, 47.20±5.90 and 50.59±4.20% in LNCaP, PC3 and DU145 cells, respectively (Fig. 1A). Colony formation in the presence of 6-Gingerol was also investigated. The colony number determined for each treatment group (1–500 µM, 6-Gingerol) was significantly reduced compared with the control group in LNCaP, PC3 and DU145 cells (Figs. 1B and S1), which suggested that 6-Gingerol inhibited cell viability and colony formation in prostate cancer cells. Furthermore, the cell survival rate of LNCaP, PC3 and DU145 cells treated with LPS was assessed (Fig. 1C). Several studies reported that LPS can enhance the metastasis and invasion in prostate and breast cancer cells (6–8). LPS (1 µg/ml) was not cytotoxic to any of the cell lines; this concentration was therefore selected to assess the adhesion, invasion, migration and EMT effects on prostate cancer cells. 6-Gingerol (100 µM) can significantly inhibit LPS-induced cell growth at 48 and 72 h (Fig. 1C). Overall, these results indicated that 6-Gingerol may exhibit cytotoxicity in a dose-dependent manner in LNCaP, PC3 and DU145 cells.
Figure 1.
6-G suppresses prostate cancer cell viability. (A) Viability of LNCaP, PC3 and DU145 cells incubated with 6-G. (B) Colony formation of LNCaP, PC3 and DU145 cells incubated with 6-G for 7 days. (C) Cell viability following treatment with 6-G (100 µM) with or without LPS (1 µg/ml). Data are presented as the mean ± SEM from three independent experiments. *P<0.05 vs. control; #P<0.05 vs. LPS. 6-G, 6-Gingerol; LPS, lipopolysaccharide.
6-Gingerol attenuates migration, invasion and adhesion in prostate cancer cells
CRPC is an aggressive disease, and it is not sensitive to medical castration with higher potential of invasion and metastasis (2). PC3 and DU145 cells are CRPC cells (7). Therefore, we selected PC3 and DU145 cells for migration and invasion assay. To investigate the mechanism of 6-Gingerol in cell migration and invasion, the wound healing and Transwell assays were performed. The results demonstrated that cell migration and invasion were significantly enhanced in LPS-induced DU145 cells. However, only cell invasion was significantly enhanced in LPS-induced PC3 cells (Figs. 2 and 3). Moreover, 6-Gingerol (10 µM) significantly inhibited migration and invasion in LPS-treated or LPS-untreated PC3 and DU145 cells at 48 h, compared with the LPS or control groups, respectively.
Figure 2.
6-G inhibits migration and invasion of LPS-stimulated and LPS-unstimulated PC3 cells. The anti-migratory and anti-invasive effects of 6-G (10 µM) with or without LPS (1 µg/ml) on PC3 cells were determined using (A) wound healing (magnification, ×100) and (B) Transwell assays (magnification, ×200). Scale bar=100 µm. Data are presented as the mean ± SEM from three independent experiments. *P<0.05 vs. control; #P<0.05 vs. LPS. 6-G, 6-Gingerol; LPS, lipopolysaccharide.
Figure 3.
6-G inhibits migration and invasion of LPS-stimulated and LPS-unstimulated DU145 cells. The anti-migration and anti-invasion effects of 6-G (10 µM) with or without LPS (1 µg/ml) on DU145 cells were determined using (A) wound healing (magnification, ×100) and (B) Transwell migration assays (magnification, ×200). Scale bar=100 µm. Data are presented as the mean ± SEM of three independent experiments. *P<0.05 vs. control; #P<0.05 vs. LPS. 6-G, 6-Gingerol; LPS, lipopolysaccharide.
Cell attachment to the extracellular matrix is important for cell metastasis in distant organs (24); therefore, the effect of 6-Gingerol on prostate cancer cell adhesion to extracellular matrix proteins was investigated. Fibronectin (5 µg/ml) significantly induced adhesion in DU145 and LNCaP cells at 2 h (Fig. 4A). 6-Gingerol (100 and 500 µM) significantly inhibited fibronectin-treated attachment at 2 h in LNCaP, DU145 and PC3 cells (Fig. 4A). The results demonstrated that LPS significantly enhanced the binding affinity of PC3, DU145 and LNCaP cells to fibronectin compared with the group treated with LPS alone at 2 h (Fig. 4B). 6-Gingerol (100, 500 µM) significantly decreased the binding affinity of LNCaP, PC3 and DU145 cells to fibronectin with or without LPS treatment compared with the LPS + fibronectin or fibronectin group, respectively at 2 h (Fig. 4). These results indicated that 6-Gingerol may have anti-invasion, anti-migration and anti-adhesion properties in prostate cancer cells.
Figure 4.
Effect of 6-G on LNCaP, PC3 and DU145 prostate cancer cell attachment on FN (5 µg/ml)-coated plates. (A) LPS-unstimulated and (B) LPS-stimulated prostate cancer cells were treated with 6-G (100 or 500 µM). Attached cells were determined using an MTT assay. *P<0.05 vs. FN; #P<0.05 vs. control; &P<0.05 vs. LPS + FN; †P<0.05 vs. LPS. 6-G, 6-Gingerol; FN, fibronectin; LPS, lipopolysaccharide.
6-Gingerol induces autophagy in prostate cancer cells
Subsequently it was determined if 6-Gingerol could induce autophagy in prostate cancer cells using western blotting to analyze Beclin-1 and LC3B protein expression levels. LC3B-II is important in autophagy and can be used as an autophagy marker (25). The results demonstrated that 6-Gingerol (10–100 µM) significantly induced LC3B-II protein expression levels in LNCaP cancer cells compared with the control (Fig. 5A). The LC3B-II protein expression levels were significantly upregulated in 6-Gingerol-treated (1–10 µM) PC3 cells. However, this was not observed in DU145 cells, due to the absence of the ATG5 protein, which results in ATG12/ATG5 conjugate deficiency (26). 6-Gingerol (10–100 µM) significantly upregulated Beclin-1 protein expression levels in LNCaP, PC3 and DU145 cells compared with the control (Fig. 5A). LY294002, a known PI3K and autophagy inhibitor, slightly enhanced 6-Gingerol cytotoxicity in LNCaP and PC3 cells (Fig. S2); however, this effect was significantly reversed in DU145 cells compared with the 6-Gingerol group (Fig. 5B). These results therefore indicated that 6-Gingerol potentially induced protective autophagy in LNCaP and PC3 cells but promoted autophagic cell death in DU145 cells. The results suggested that 6-Gingerol induced autophagy by regulating LC3B-II and Beclin-1 protein expression levels in LNCaP and PC3 cells. Moreover, 6-Gingerol also induced autophagy by inducing Beclin-1 and LC3B-I but without LC3B-II protein expression in DU145 cells.
Figure 5.
6-G induces autophagy in prostate cancer cells. (A) Prostate cancer cells were treated with 6-G (1–100 µM) for 24 h. The protein expression levels of Beclin-1 and LC3B were determined via western blotting. (B) 6-Gingerol-treated cell viability in the presence or absence of LY294002 (10 µM) in DU145 cells incubated for 24 and 48 h. Semi-quantification of Beclin-1 and LC3B protein expression levels were performed using image analysis in (C) PC3, (D) LNCaP and (E) DU145 cells. *P<0.05 vs. control; &P<0.05 vs. 6-G (100 µM). 6-G, 6-Gingerol.
6-Gingerol suppresses EMT-related protein expression in prostate cancer cells
EMT serves a significant role in cancer progression and metastasis, a mechanism which LPS can trigger and enhance (6–8). The protein expression levels of E-cadherin, N-cadherin, Vimentin and ZO-1 were examined following 6-Gingerol (1–100 µM) treatment for 24 h in LNCaP, PC3 and DU145 cells. The results demonstrated that E-cadherin and ZO-1 were significantly upregulated in 6-Gingerol-treated (10–100 µM) prostate cancer cells compared with the control (Fig. 6); however, N-cadherin and Vimentin were downregulated in 6-Gingerol-treated PC3 and LNCaP cells. The protein expressions of N-cadherin were not significantly inhibited by 6-Gingerol (1–100 µM) treatment for 24 h in DU145 cells. Cell invasion and migration were significantly induced after LPS treatment in DU145 cells. Therefore, DU145 cells were selected for examining the underlying mechanism of action of EMT in LPS-treated DU145 cells. Furthermore, the results indicated that LPS significantly induced N-cadherin and Vimentin protein expression levels in DU145 cells at 48 h compared with the control (Fig. 7A and C). In addition, 6-Gingerol did not markedly increased E-cadherin protein expression levels, whereas it significantly downregulated N-cadherin and Vimentin protein expression levels in the LPS + 6-Gingerol group compared with the LPS group. The results also demonstrated that the protein expression levels of LC3B-I were significantly decreased in LPS-stimulated DU145 cells compared with the control. 6-Gingerol (100 µM) reversed the protein expression levels of LC3B-I in LPS-stimulated DU145 cells. These data indicated that LPS potentially stimulated EMT and that 6-Gingerol may reverse these effects on EMT in LPS-treated prostate cancer cells.
Figure 6.
6-G inhibits the epithelial-mesenchymal transition in prostate cancer cells. Prostate cancer cells were treated with 6-G (1–100 µM) for 24 h. The protein expression levels of E-cadherin, N-cadherin, Vimentin and ZO-1 were analyzed via western blotting. *P<0.05 vs. control. 6-G, 6-Gingerol; ZO-1, zonula occludens-1.
Figure 7.
Effects of 6-G treatment on autophagy, the epithelial-mesenchymal transition and ferroptosis in LPS-stimulated and LPS-unstimulated prostate cancer cells. (A) DU145 cells were treated with 6-G (100 µM) with or without LPS (1 µg/ml) for 48 h. Western blotting was performed to determine the protein expression levels of E-cadherin, N-cadherin, Vimentin and LC3B-I. (B) NRF2 and GPX4 protein expression levels following 6-G treatment for 24 h were determined via western blotting. (C) Semi-quantification of E-cadherin, N-cadherin, Vimentin and LC3B-I protein expression levels was performed using image analysis. (D) Semi-quantification of GPX4 and NRF2 protein expression levels was performed using image analysis. *P<0.05 vs. control; #P<0.05 vs. LPS. 6-G, 6-Gingerol; LPS, lipopolysaccharide; GPX4, glutathione peroxidase 4; NRF2, nuclear factor erythroid 2-related factor 2.
6-Gingerol treatment induces ferroptosis
Ferroptosis is associated with ROS production, which leads to decreased cellular GSH levels (27). GPX4 is an enzyme that belongs to the family of GPXs and GPX4 inactivation can promote ferroptosis (28). Therefore, the role of ROS, GSH, GPX4 and NRF2 protein expression in prostate cancer cells was determined. GPX4 and NRF2 protein expression levels were significantly downregulated after 24 h of 6-Gingerol (100 µM) treatment in LNCaP, PC3 and DU145 cells (Fig. 7B and D). NRF2 protein expression levels were increased after 6-Gingerol (1–10 µM) treatment in PC3 and DU145 cells, but this was not observed in LNCaP cells. PC3 and DU145 are castration-resistant prostate cancer cells, and LNCaP is androgen-dependent prostate cancer cell line (18,29). This might slightly increase NRF2 levels after low concentration of 6-Gingerol treatment in PC3 and DU145 cells because of castration-resistant prostate cancer cells. Furthermore, ROS levels were significantly increased following 6-Gingerol treatment in LNCaP, PC3 and DU145 cells compared with the control. Notably, this effect was significantly attenuated by pre-treatment with ferrostatin-1, compared with the 6-Gingerol only group (Figs. 8A and S3).
Figure 8.
6-G triggers ferroptosis in LNCaP, PC3 and DU145 cells. (A) ROS levels in prostate cancer cells following 6-G treatment with or without Fer-1 (5 µM) for 24 h. (B) Cytotoxicity of 6-G (100 µM) with or without Fer-1 (5 µM) for 24 and 48 h in prostate cancer cells. (C) GSH concentration in prostate cancer cells following 6-G treatment with or without Fer-1 (5 µM) for 24 h. (D) GPX4 protein expression levels following 6-G (100 µM) treatments with or without Fer-1 (5 µM) for 24 h in DU145 cells. *P<0.05 vs. control; #P<0.05 vs. 6-G (100 µM). 6-G, 6-Gingerol; ROS, reactive oxygen species; Fer-1, ferrostatin-1; GSH, glutathione.
To further determine the effect of 6-Gingerol on cell death, ferrostatin-1, an effective ferroptosis inhibitor, was used. The results demonstrated that ferrostatin-1 significantly alleviated a decrease in cell viability in LNCaP, PC3 and DU145 cells at 48 h in cells treated with 6-Gingerol (100 µM) compared with the 6-Gingerol group (Fig. 8B). GSH levels were also significantly reduced after 6-Gingerol treatment (10–100 µM) compared with the control; however, this effect was significantly attenuated by pre-treatment with ferrostatin-1 (5 µM) compared with the 6-Gingerol group (100 µM) (Fig. 8C). GPX4 protein expression levels were attenuated following 6-Gingerol (100 µM) treatment for 24 h in DU145 cell. The expression was significantly increased in ferrostatin-1 pre-treatment 6-Gingerol-treated (100 µM) DU145 cells compared with the 6-Gingerol group (Fig. 8D). These results indicated that cell death may be mediated by a ferroptosis mechanism. Furthermore, these data indicated that 6-Gingerol may induce ROS accumulation and ferroptosis; therefore, ferroptosis may be a potential mechanism, induced by 6-Gingerol, against prostate cancer cell proliferation.
Discussion
6-Gingerol has been reported to induce apoptosis in numerous types of cancer cells, including breast cancer, colon cancer, prostate cancer and cervical cancer cells (21,30–32). In addition, it may regulate both multidrug resistance-associated protein 1 and glutathione S-transferase in docetaxel-resistant prostate cancer cells (21). To the best of our knowledge, no study has focused on the anti-migratory and anti-invasive activity of 6-Gingerol in prostate cancer cells. In the present study, it was reported that 6-Gingerol affected human androgen-dependent (LNCaP) and castrate-resistant (DU145 and PC3) prostate cancer cells by inducing autophagy and ferroptosis. The results also demonstrated that 6-Gingerol significantly inhibited cell migration and invasion via the regulation of EMT-related proteins in prostate cancer cells.EMT serves a significant role in cancer progression, whereby epithelial cells lose cell polarity and are transformed into cells with a mesenchymal phenotype, which exhibit increased migratory and invasive abilities in combination with reduced intracellular adhesion (33). EMT is also associated with cancer stem cell-like properties and chemotherapy drug resistance (4). Therefore, a therapeutic agent that can effectively inhibit the EMT process may be a potential anti-metastatic strategy. Cadherins, named for ‘calcium-dependent adhesion’, serve a key role in adherens junctions (34). A loss in E-cadherin expression can result in the loss of contact inhibition, and increase cell motility and invasion (35). Notably, N-cadherin is expressed in mesenchymal cells and is overexpressed in cancer cells (36). Vimentin is an intermediate filament protein, which is a cytoskeletal component in mesenchymal cells (37). In the present study, it was demonstrated that E-cadherin and ZO-1 protein expression levels were significantly upregulated following 6-Gingerol treatment in prostate cancer cells, whereas the mesenchymal markers, Vimentin and N-cadherin were significantly decreased following 6-Gingerol treatment in the PC3 and LNCaP cell lines. Our previous study reported that LPS can enhance cell migration, invasion and inflammation in prostate cancer cells (8). LPS is known to induce EMT in prostate and breast cancer cells, which results in metastasis (7,38). In the present study, the results demonstrated that LPS stimulated EMT progression by significantly increasing Vimentin and N-cadherin and did not markedly attenuate E-cadherin protein expression levels in DU145 cells. Cell invasion and migration were significantly induced following LPS treatment, whereas 6-Gingerol significantly suppressed cell migration and invasion, and EMT by reversing this pattern of EMT protein expression levels in LPS-treated DU145 cells.Autophagy is a form of cell death that can remove mis-folded proteins and maintain cellular homeostasis under stressful conditions; notably, excess autophagy can also result in cell death (39). Therefore, the induction or inhibition of autophagy is considered to be a potential novel strategy for the treatment of cancer (39). In the present study, 6-Gingerol significantly induced LC3B conversion and Beclin-1 protein expression in prostate cancer cells. However, autophagy inhibitor LY294002 increased 6-Gingerol-induced cell death in PC3 and LNCaP cells. Previous studies have reported that autophagy serves a cytoprotective role against apoptosis (39,40). These results revealed that autophagy induction of 6-Gingerol might protect PC3 and LNCaP cells from cytotoxicity effects. However, cell viability was increased following 6-Gingerol combined with LY294002 treatment in DU145 cells. Protective autophagy (PC3 and LNCaP) and autophagic cell death (DU145) were observed after 6-Gingerol treatment in prostate cancer cells.Recent studies have demonstrated that ferroptosis is important in the regulation of tumor cell proliferation, including in breast, lung and prostate cancer (41–43). Therefore, ferroptosis may be a potential novel strategy and therapeutic target for the treatment of cancer. Ferroptosis results from the depletion of GSH, GPX4 inactivation and intracellular ROS accumulation (44). In the present study, 6-Gingerol significantly decreased the levels of GPX4 and GSH, and significantly elevated ROS accumulation in PC3, DU145 and LNCaP cells. Previous studies have reported that 6-Gingerol-induced ROS production is accompanied by apoptosis in gastric cancer, human epidermoid carcinoma and myeloid leukemia cells (45–47).The results of the present study demonstrated that 6-Gingerol may have significantly induced ROS production via a ferroptosis mechanism in prostate cancer cells and that pretreatment with the ferroptosis inhibitor, ferrostatin-1, significantly reversed 6-Gingerol-induced ferroptosis. NRF2 is a transcription factor that regulates signaling pathways in response to oxidative stress. Inhibition or knockdown of the NRF2 gene has been shown to enhance ferroptosis that results in decreased GSH synthesis and GPX4 inhibition (48,49). The present study demonstrated that 6-Gingerol (100 µM) significantly decreased NRF2 protein expression levels in prostate cancer cells. Taken together, these data suggested that 6-Gingerol may promote ferroptosis, which could be beneficial for the treatment of prostate cancer. Furthermore, these results indicated that ferroptosis potentially serves an important role in mediating cell death in DU145 cells treated with 6-Gingerol.6-Gingerol is a flavonoid antioxidant that is enriched in fresh ginger. Numerous studies have reported that 6-Gingerol has anticancer and anti-inflammatory effects (20,50–53). The present study provided new evidence that 6-Gingerol may have potential anti-metastatic and anticancer activities in prostate cancer cells (Fig. 9). 6-Gingerol significantly regulated EMT-related protein expression levels in LPS-stimulated and LPS-unstimulated prostate cancer cells. Furthermore, 6-Gingerol may trigger autophagy and ferroptosis, which suggested that both mechanisms may serve pivotal roles in regulating cell survival. In summary, 6-Gingerol may be considered an important novel therapeutic agent for the prevention and treatment of prostate cancer as a result of its numerous pharmacological activities. Our study demonstrated that 6-Gingerol can suppress migration, invasion and cell survival in CRPC, and androgen-dependent prostate cancer cells. In vivo studies are needed to verify these results in the future.
Figure 9.
Diagram demonstrating the inhibition of cell proliferation and EMT in prostate cancer cells following 6-Gingerol treatment. In the present study, 6-Gingerol induced autophagy and ferroptosis. 6-Gingerol also reversed the EMT in LPS-treated and LPS-untreated prostate cancer cells. EMT, epithelial-mesenchymal transition; LPS, lipopolysaccharide; TLR4, toll-like receptor 4; NRF2, nuclear factor erythroid 2-related factor 2; GSH, glutathione; GPX4, glutathione peroxidase 4; ZO-1, zonula occludens-1; HO-1, heme oxygenase-1; GS-SG, oxidized glutathione.
Authors: E K Radhakrishnan; Smitha V Bava; Sai Shyam Narayanan; Lekshmi R Nath; Arun Kumar T Thulasidasan; Eppurathu Vasudevan Soniya; Ruby John Anto Journal: PLoS One Date: 2014-08-26 Impact factor: 3.240
Authors: Jie Li; Feng Cao; He-Liang Yin; Zi-Jian Huang; Zhi-Tao Lin; Ning Mao; Bei Sun; Gang Wang Journal: Cell Death Dis Date: 2020-02-03 Impact factor: 8.469
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