K Wang1, J Zhang1, M Deng1, Y Ju1, M Ouyang1. 1. Department of Gastrointestinal Surgery, Shunde Hospital, Southern Medical University, Foshan 528308, China.
Abstract
OBJECTIVE: To investigate the expression and gene function of methyltransferase-like protein 27 (METTL27) in colon cancer, its association with immune infiltration and its prognostic significance. METHODS: We analyzed the expression levels of METTL27 in 33 cancers using R language and identified METTL27 as a differential gene in colon cancer. The related signaling pathways of METTL27 were analyzed by gene functional annotation and enrichment. SsGSEA algorithm was used to analyze immune infiltration, and logistic analysis was used to evaluate the correlation between METTL27 expression and clinicopathological features of the patients. Kaplan-meier analysis, univariate and multivariate Cox regression analysis were performed to construct a nomogram for evaluating the correlation between METTL27 expression and clinical prognosis. The expression level of METTL27 was further verified in colorectal cancer cell lines and 16 clinical specimens of colorectal cancer tissues using qPCR and Western blotting. RESULTS: METTL27 was highly expressed in 21 cancers, and its expression was significantly higher in colon cancer than in adjacent tissues (P < 0.001). METTL27-related genes were identified by differential analysis, and functional annotation revealed that METTL27 was significantly enriched in transmembrane transport and lipid metabolism, and 5 related signaling pathways were identified by GSEA. METTL27 expression was negatively correlated with different T helper cells and central memory T cells (P < 0.001). The patients with a high METTL27 mRNA expression had a poor survival outcome. Cox regression analysis showed that METTL27 expression was an independent prognostic factor of the overall survival. The expression level of METTL27 was significantly higher in the colorectal cancer cell line than in normal cells (P < 0.05). CONCLUSION: METTL27 is overexpressed in colon cancer and is associated with a poor prognosis of the patients. A high expression of METTL27 showed is associated less T cell immune infiltration, suggesting the potential of METTL27 as a prognostic marker of colon cancer.
OBJECTIVE: To investigate the expression and gene function of methyltransferase-like protein 27 (METTL27) in colon cancer, its association with immune infiltration and its prognostic significance. METHODS: We analyzed the expression levels of METTL27 in 33 cancers using R language and identified METTL27 as a differential gene in colon cancer. The related signaling pathways of METTL27 were analyzed by gene functional annotation and enrichment. SsGSEA algorithm was used to analyze immune infiltration, and logistic analysis was used to evaluate the correlation between METTL27 expression and clinicopathological features of the patients. Kaplan-meier analysis, univariate and multivariate Cox regression analysis were performed to construct a nomogram for evaluating the correlation between METTL27 expression and clinical prognosis. The expression level of METTL27 was further verified in colorectal cancer cell lines and 16 clinical specimens of colorectal cancer tissues using qPCR and Western blotting. RESULTS: METTL27 was highly expressed in 21 cancers, and its expression was significantly higher in colon cancer than in adjacent tissues (P < 0.001). METTL27-related genes were identified by differential analysis, and functional annotation revealed that METTL27 was significantly enriched in transmembrane transport and lipid metabolism, and 5 related signaling pathways were identified by GSEA. METTL27 expression was negatively correlated with different T helper cells and central memory T cells (P < 0.001). The patients with a high METTL27 mRNA expression had a poor survival outcome. Cox regression analysis showed that METTL27 expression was an independent prognostic factor of the overall survival. The expression level of METTL27 was significantly higher in the colorectal cancer cell line than in normal cells (P < 0.05). CONCLUSION: METTL27 is overexpressed in colon cancer and is associated with a poor prognosis of the patients. A high expression of METTL27 showed is associated less T cell immune infiltration, suggesting the potential of METTL27 as a prognostic marker of colon cancer.
Authors: Sofia S Mariasina; Chi-Fon Chang; Olga A Petrova; Sergey V Efimov; Vladimir V Klochkov; Olga I Kechko; Vladimir A Mitkevich; Petr V Sergiev; Olga A Dontsova; Vladimir I Polshakov Journal: FEBS J Date: 2020-04-20 Impact factor: 5.542
Authors: Mark R Sullivan; Katherine R Mattaini; Emily A Dennstedt; Anna A Nguyen; Sharanya Sivanand; Montana F Reilly; Katrina Meeth; Alexander Muir; Alicia M Darnell; Marcus W Bosenberg; Caroline A Lewis; Matthew G Vander Heiden Journal: Cell Metab Date: 2019-03-21 Impact factor: 27.287
Authors: Oxana Dmitrieva-Posocco; Amiran Dzutsev; David F Posocco; Vivianty Hou; Wuxing Yuan; Vishal Thovarai; Ilgiz A Mufazalov; Matthias Gunzer; Igor P Shilovskiy; Musa R Khaitov; Giorgio Trinchieri; Ari Waisman; Sergei I Grivennikov Journal: Immunity Date: 2019-01-15 Impact factor: 43.474
Authors: Ceren Ozkul; Victoria E Ruiz; Thomas Battaglia; Joseph Xu; Claire Roubaud-Baudron; Ken Cadwell; Guillermo I Perez-Perez; Martin J Blaser Journal: Genome Med Date: 2020-07-25 Impact factor: 11.117
Authors: Ivan Laptev; Ekaterina Shvetsova; Sergey Levitskii; Marina Serebryakova; Maria Rubtsova; Victor Zgoda; Alexey Bogdanov; Piotr Kamenski; Petr Sergiev; Olga Dontsova Journal: Nucleic Acids Res Date: 2020-06-23 Impact factor: 16.971
Authors: Sofia S Mariasina; Chi-Fon Chang; Tsimafei L Navalayeu; Anastasia A Chugunova; Sergey V Efimov; Viktor G Zgoda; Vasily A Ivlev; Olga A Dontsova; Petr V Sergiev; Vladimir I Polshakov Journal: Front Mol Biosci Date: 2022-06-15