Literature DB >> 3552733

Glucose stimulates the biosynthesis of rat I and II insulin to an equal extent in isolated pancreatic islets.

C J Rhodes, C A Lucas, P A Halban.   

Abstract

The effects of glucose on insulin biosynthesis were studied by measuring the incorporation of radiolabelled amino acids into proinsulin/insulin in isolated rat islets. The islets were pulse labelled for 15 min with [3H]leucine (present in rat insulin I and II) or [35S]methionine (unique to rat insulin II) and then incubated for a 165 min post-label (chase) period during which the majority of labelled proinsulin was converted to insulin but under conditions whereby greater than 95% of radiolabelled proinsulin or insulin was retained in the islets. The newly synthesized, labelled, insulin was analyzed by high performance liquid chromatography. Rat I and II insulin biosynthesis was stimulated by 16.7 mM glucose to the same extent.

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Year:  1987        PMID： 3552733     DOI： 10.1016/0014-5793(87)80137-0

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  8 in total

1.  Partial diversion of a mutant proinsulin (B10 aspartic acid) from the regulated to the constitutive secretory pathway in transfected AtT-20 cells.

Authors:  D J Gross; P A Halban; C R Kahn; G C Weir; L Villa-Komaroff
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

2.  High-performance liquid chromatography analysis of circulating insulins distinguishes between endogenous insulin production (a potential pitfall with streptozotocin diabetic rats) and islet xenograft function.

Authors:  D Chicheportiche; S Darquy; J Lepeintre; F Capron; P A Halban; G Reach
Journal:  Diabetologia       Date:  1990-08       Impact factor: 10.122

3.  Islet beta cell failure in the 60% pancreatectomised obese hyperlipidaemic Zucker fatty rat: severe dysfunction with altered glycerolipid metabolism without steatosis or a falling beta cell mass.

Authors:  V Delghingaro-Augusto; C J Nolan; D Gupta; T L Jetton; M G Latour; M Peshavaria; S R Murthy Madiraju; E Joly; M-L Peyot; M Prentki; J Leahy
Journal:  Diabetologia       Date:  2009-03-18       Impact factor: 10.122

4.  Adaptation to supraphysiologic levels of insulin gene expression in transgenic mice: evidence for the importance of posttranscriptional regulation.

Authors:  B Schnetzler; G Murakawa; D Abalos; P Halban; R Selden
Journal:  J Clin Invest       Date:  1993-07       Impact factor: 14.808

5.  Differential inhibitory action of cationic amino acids on protein synthesis in pancreatic rat islets.

Authors:  C Patzelt
Journal:  Diabetologia       Date:  1988-04       Impact factor: 10.122

6.  The intracellular handling of insulin-related peptides in isolated pancreatic islets. Evidence for differential rates of degradation of insulin and C-peptide.

Authors:  C J Rhodes; P A Halban
Journal:  Biochem J       Date:  1988-04-01       Impact factor: 3.857

Review 7.  Proinsulin processing in the regulated and the constitutive secretory pathway.

Authors:  P A Halban
Journal:  Diabetologia       Date:  1994-09       Impact factor: 10.122

8.  A genetic screen identifies Crat as a regulator of pancreatic beta-cell insulin secretion.

Authors:  Dassine Berdous; Xavier Berney; Ana Rodriguez Sanchez-Archidona; Maxime Jan; Clara Roujeau; Isabel C Lopez-Mejia; Randall Mynatt; Bernard Thorens
Journal:  Mol Metab       Date:  2020-04-13       Impact factor: 7.422
  8 in total

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