The Fao cell. A tissue culture model for lipoprotein synthesis and secretion. I. Characterization of the system.

In this study we found that a differentiated cultured rat hepatocyte cell line, Fao, synthesizes and secretes lipoproteins qualitatively similar to those synthesized by the rat hepatocyte in vivo, but quantitatively differing considerably in apoprotein composition and density distribution. Immunoprecipitation demonstrates that all the major apoproteins are synthesized, including both forms of apoB, apoE and apoA-I. Particles of all density classes are formed, apoB associating with the lighter particles and apoA-I with the heavier. ApoE is a major apoprotein in all but the lightest density classes. The general finding is that most particles formed have a density exceeding 1.10 g/ml, while very few of the lighter, apoB-containing particles form, probably because the normal growth medium of the cultured cells is lipid-poor as compared with rat serum. In ref. [20] we show that the composition of the lipoproteins synthesized can be effectively modulated by lipid depletion and lipid supplementation of the growth medium.