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Literature DB >> 355262

Localization of actin in Dictyostelium amebas by immunofluorescence.

Abstract

Antibody prepared against avian smooth muscle actin has been used to localize actin in the slime mold, Dictyostelium discoideum. The distribution of actin in migrating cells is different from that in feeding cells. Migrating amebas display fluorescence primarily in advancing regions whereas feeding amebas show uniform fluorescence throughout. The reaction is specific for actin since the fluorescence observed is blocked when the antibody is absorbed by actin purified from avian skeletal muscle, human platelets, and Dictyostelium. These results, in addition to describing the distribution of actin in D. discoideum, demonstrate that actins from these diverse sources share at least one common antigenic determinant.

Entities: Species

Mesh：

Substances：
Actins
Actomyosin

Year: 1978 PMID： 355262 PMCID： PMC2110147 DOI： 10.1083/jcb.77.3.714

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

17 in total

1. Orientation and rate of locomotion of individual amebas in the life cycle of the cellular slime mold Dictyostelium mucoroides.

Authors: E W SAMUEL
Journal: Dev Biol Date: 1961-06 Impact factor: 3.582

2. Cell communication by periodic cyclic-AMP pulses.

Authors: G Gerisch; D Hülser; D Malchow; U Wick
Journal: Philos Trans R Soc Lond B Biol Sci Date: 1975-11-06 Impact factor: 6.237

3. The contractile basis of amoeboid movement III. Structure and dynamics of motile extracts and membrane fragments from Dictyostelium discoideum and Amoeba proteus.

Authors: D L Taylor; J S Condeelis; J A Rhodes
Journal: Prog Clin Biol Res Date: 1977

4. Supramolecular forms of actin from amoebae of Dictyostelium discoideum.

Authors: J A Spudich; R Cooke
Journal: J Biol Chem Date: 1975-09-25 Impact factor: 5.157

5. Inducation of antibody against actin from myxomycete plasmodium and its properties.

Authors: K Owaribe; S Hatano
Journal: Biochemistry Date: 1975-07 Impact factor: 3.162

6. Actin is the naturally occurring inhibitor of deoxyribonuclease I.

Authors: E Lazarides; U Lindberg
Journal: Proc Natl Acad Sci U S A Date: 1974-12 Impact factor: 11.205

7. A comparison of the distribution of actin and tubulin in the mammalian mitotic spindle as seen by indirect immunofluorescence.

Authors: W Z Cande; E Lazarides; J R McIntosh
Journal: J Cell Biol Date: 1977-03 Impact factor: 10.539

8. The role of actin in the temperature-dependent gelation and contraction of extracts of Acanthamoeba.

Authors: T D Pollard
Journal: J Cell Biol Date: 1976-03 Impact factor: 10.539

9. Fluorescent antibody localization of myosin in the cytoplasm, cleavage furrow, and mitotic spindle of human cells.

Authors: K Fujiwara; T D Pollard
Journal: J Cell Biol Date: 1976-12 Impact factor: 10.539

10. Structural and biochemical aspects of cell motility in amebas of Dictyostelium discoideum.

Authors: B S Eckert; R H Warren; R W Rubin
Journal: J Cell Biol Date: 1977-02 Impact factor: 10.539

11 in total

1. Studies on the antigenic sites of actin: a comparative study of the immunogenic crossreactivity of invertebrate actins.

Authors: H G De Couet
Journal: J Muscle Res Cell Motil Date: 1983-08 Impact factor: 2.698

2. Association of the cyclic AMP chemotaxis receptor with the detergent-insoluble cytoskeleton of Dictyostelium discoideum.

Authors: N J Galvin; D Stockhausen; B L Meyers-Hutchins; W A Frazier
Journal: J Cell Biol Date: 1984-02 Impact factor: 10.539

3. Localization of actin and myosin for the study of ameboid movement in Dictyostelium using improved immunofluorescence.

Authors: S Yumura; H Mori; Y Fukui
Journal: J Cell Biol Date: 1984-09 Impact factor: 10.539

4. Association of creatine phosphokinase with the cytoskeleton of cultured mammalian cells.

Authors: B S Eckert; S J Koons; A W Schantz; C R Zobel
Journal: J Cell Biol Date: 1980-07 Impact factor: 10.539

5. Location of actin, myosin, and microtubular structures during directed locomotion of Dictyostelium amebae.

Authors: S Rubino; M Fighetti; E Unger; P Cappuccinelli
Journal: J Cell Biol Date: 1984-02 Impact factor: 10.539

6. The Dictyostelium discoideum 30,000-dalton protein is an actin filament-bundling protein that is selectively present in filopodia.

Authors: M Fechheimer
Journal: J Cell Biol Date: 1987-06 Impact factor: 10.539

7. Ligand-induced changes in the location of actin, myosin, 95K (alpha-actinin), and 120K protein in amebae of Dictyostelium discoideum.

Authors: J M Carboni; J S Condeelis
Journal: J Cell Biol Date: 1985-06 Impact factor: 10.539

8. Abundance, relative gelation activity, and distribution of the 95,000-dalton actin-binding protein from Dictyostelium discoideum.

Authors: J Brier; M Fechheimer; J Swanson; D L Taylor
Journal: J Cell Biol Date: 1983-07 Impact factor: 10.539

9. Filopodelike projections induced with dimethyl sulfoxide and their relevance to cellular polarity in Dictyostelium.

Authors: S Yumura; Y Fukui
Journal: J Cell Biol Date: 1983-03 Impact factor: 10.539

10. Dynamics of nuclear actin bundle induction by dimethyl sulfoxide and factors affecting its development.

Authors: Y Fukui; H Katsumaru
Journal: J Cell Biol Date: 1980-01 Impact factor: 10.539