Clostridium perfringens and Escherichia coli Bacteremia in a Patient with Acute Obstructive Suppurative Cholangitis: A Case Report and Review of the Literature.

BACKGROUND Clostridium perfringens (CP), one of several clostridial species gram-positive bacteria, is a major cause of animal necrosis enteritis and traumatic gangrene. In some reports, CP can cause acute emphysematous cholecystitis in patients with biliary tract infections. However, C. perfringens combined with other aerobic bacteria (eg, E. coli) in bloodstream co-infection is extremely rare and often fatal. Herein, we present a case of co-infection to underscore this unusual situation so that clinicians can adequately evaluate and treat patients in time. CASE REPORT A 74-year-old man presented to the Emergency Department half a day after the onset of acute abdominal pain accompanied by nausea, vomiting, and chills. The patient was admitted, following development of jaundice, chills, high fever, confusion, and shock. Computed tomography (CT) revealed that the patient had cholangiectasis with acute obstructive suppurative cholangitis (AOSC). We subsequently performed percutaneous transhepatic gallbladder drainage surgery combined with antibiotics, including ceftriaxone, levofloxacin, and metronidazole. C. perfringens and Escherichia coli infections were identified by in vitro blood culture. Fortunately, the patient responded favorably to treatment in our hospital and was cured within 1 week. CONCLUSIONS We report a rare case of C. perfringens and E. coli bloodstream co-infection in a patient with AOSC. We suggest that anaerobic and aerobic co-infection should be considered in future clinical diagnoses. Effective antibiotic treatment combined with surgical drainage is crucial if mixed infection occurs.

Background

Clostridium perfringens (CP) is a common anaerobic gram-positive bacterium that can be isolated from the soil and from the human gastrointestinal and urogenital tracts [1]. CP can cause septicemia through release of more than 20 different toxins, including α-toxin, which can induce hemolysis, and with poor prognosis [2]. Moreover, the released toxin is capable of causing toxinosis in the host, even if the bacteria are eliminated. For survival, control of potentially fatal hemolysis using appropriate antibiotics and surgery is necessary, and effective therapy should be started before getting the result of blood cultures.

Rare cases of CP in bloodstream infection have been described in the medical literature and the mortality rate is up to 59.7% (74/124) according to recent studies [3,4]. CP infections show broad clinical manifestations, including gangrene, massive intravascular hemolysis, and multi-organ failure from septic shock. Additionally, CP can occur as co-infection with other pathogens, which dramatically increases patient risk.

Herein, we present a case report of a patient with acute obstructive suppurative cholangitis with C. perfringens and E. coli co-infection. In addition, we provide an overview of all reported CP infections since 1990 in humans with gastrointestinal disease and co-infection of CP with other pathogens.

Case Report

On 30 Oct 2017, a 74-year-old man presented to the Emergency Department of Shanghai Pudong New Area Gongli Hospital after he had begun to experience abdominal pain for half a day. He was febrile (38.7°C) and had a pulse rate of 113 times/min, breathing rate of 20 times/min, and blood pressure of 112/69 mmHg. A repeat computed tomography (CT) scan of the abdomen was obtained, which showed a significant gallbladder enlargement and dilated common bile duct, indicating the possibility of bile duct calculus (). A blood test demonstrated a high count of WBC and neutrophils, as well as an abnormal liver function index (). Above all, the patient was diagnosed with a biliary tract infection and was taken to the Department of Gastroenterology. After clinical admission, the patient completed relevant examinations and fasting. A total of 4 bilateral blood culture flasks (aerobic culture and anaerobic culture) were sent to the Clinical Bacteriology Department for examination. The treatment course was carefully determined and administered, which include the anti-infective drugs ceftazidime and levofloxacin. However, the symptoms worsened, including persistent abdominal pain, jaundice, chills, and confusion, the temperature rose to 40.4°C, heart rate of 100 beats/min, and blood pressure of 95/50 mmHg. Due to concern about septic shock and acute obstructive suppurative cholangitis, the patient was immediately transferred to the Department of Surgery at 8 PM. The antibiotic treatment regimen was adjusted to ceftriaxone and metronidazole sodium chloride injection. The patient underwent percutaneous transhepatic gallbladder drainage the next day, and his body temperature dropped to 36.5°C and remained normal for 72 h. On 1 Nov, the patient underwent magnetic resonance cholangiogram cryptography (MRCP), which showed the gallbladder was contracted and there were fewer common bile duct calculi ().

Both anaerobic and aerobic flasks cultivational tests were positive after 12 h of incubation. The specific identification steps were performed. Gas was detected in the anaerobic flask, and direct image microscopic examination found coarse gram-positive bacilli, implying the possibility of anaerobic bacilli infection and the clinician was notified immediately (). Bacterial colonies with off-white, oblique, and hemolysis rings were detected on the plate after 24 h of anaerobic culture. Staining of colony smears showed blunt-rounded gram-positive bacilli (). The colonies were selected and subsequently identified as Clostridium perfringens by MALDI TOF MS (). Various off-white, smooth, and moist colonies could be seen on the aerobic plate after 24-h culture, suggesting a gram-negative bacillus, which was subsequently identified as Escherichia coli using the same automatic microbial identification, and the antimicrobial susceptibility was analyzed using the VITEK Compact-2 automatic system.

On 4 Nov, the patient continued to improve and was discharged from the hospital. A review CT after 1 week showed improved outcomes (). Three months later, the patient underwent the surgical combined treatment of cholecystectomy and choledochotomy by choledochoscopy with T-tube drainage.

We searched the literature for all cases of C. perfringens bloodstream infection in humans with gastrointestinal symptoms disease since 1990 and the papers we found are listed in . We also searched cases caused by C. perfringens following co-infection with other pathogens and found only 7 reported cases in humans or other species (). Among these 7 cases, only 1 patient, who was infected with C. perfringens following E. coli, has been reported in 1993, whose death occurred as a result of a subsequent urea-instillation abortion [9]. For other species (eg, pigs, goats, and dogs), all of them died due to co-infection. To the best of our knowledge, this is the first reported case of C. perfringens and E. coli mixed infection in a patient with acute obstructive suppurative cholangitis who survived after gallbladder drainage surgical treatment.

Discussion

C. perfringens sepsis is rare, with only a few cases described in the literature, but it is usually associated with serious hemolysis and high mortality [5]. The α-toxin is produced by C. perfringens, which mainly causes severe intravascular hemolysis and deficiency of blood, associated with its role of degradation of phospholipids in the red blood cell membrane and the destruction of platelets, massive tissue necrosis, gas generation, and gangrene [6]. Based on previous reports, the median time from admission to death is only 8 h during CP blood infection [7]. However, early diagnosis of infection is usually difficult due to the high morbidity and short survival time.

Therefore, it is necessary to establish effective guidelines for the treatment of C. perfringens infection.

The biliary tract infections, including cholecystitis and cholangitis, are usually secondary to predisposing factors leading to bacteremia or sepsis. In clinical practice, some biliary tract microbial agents are gram-negative bacteria, as well as fungi. A previous study showed that 60.9% of patients have bacterial infections, of which E. coli infection accounted for 44.4%, followed by K. pneumoniae, which accounted for 27.3% [8]. Mixed anaerobic-aerobic infections occur in a variety of locations, including the abdomen, pelvis, and soft tissues [9]. However, bacteremia caused by C. perfringens and E. coli is rare and can induce a more serious and fatal disease [10]. In this circumstance, the necessity of treatment by surgical drainage and appropriate antibiotics has been adequately determined in clinical studies [11].

In the present case, the patient was diagnosed with acute obstructive suppurative cholangitis based on clinical symptoms and imaging. Subsequently, the patient received relevant assessment after admission and we adjusted treatment strategies according to the clinical report. Fortunately, the patient did not have serious clinical outcomes, although he also had clinical symptoms such as high fever and cognitive deficits. A positive clinical response often depends on timely and effective treatment. The bloodstream infectious source is most likely via a damaged biliary tract caused by inflammation. Although the causes and mechanisms of this bloodstream mixed infection are still unclear, a possible explanation is a decreased ratio of oxygen in the blood due to E. coli infection through a biological mechanism, and this reduced level of oxygen increases the risk of facultative anaerobic C. perfringens proliferation. In the case of emphysema cholecystitis caused by C. perfringens, a large amount of gas is generally seen in the gallbladder on abdominal CT. However, we did not find this symptom in our patient, which may be related to effective treatment that had a bacteriostatic effect. Moreover, after admission, the diagnosis of acute obstructive suppurative cholangitis was confirmed by a series of typical clinical symptoms and choledocholithiasis. Timely treatment such as early empirical use of metronidazole and levofloxacin and gallbladder drainage-controlled infection prevented further deterioration. The patient’s body temperature returned to normal and no fever recurred. Blood cultures were negative after antibiotics were administered, indicating a bacterial infection causing transient bacteremia.

Limitations of this case report include the absence of anaerobic culture of gallbladder drainage fluid, leading to the inability to identify the source of bloodstream infection, and insufficient evidence of C. perfringens and E. coli co-infection. Imaging did not show abdominal gas, which was inconsistent with emphysema cholecystitis. However, we obtained and identified C. perfringens in both sides of anaerobic blood culture bottles to confirm our conclusion, which highlights the need to collect blood without contamination, and the collection should occur before antibiotics treatment.

Conclusions

We presented a rare case suggesting that bloodstream infection by C. perfringens following E. coli should be considered during acute inflammation. When this mixed anaerobic and aerobic infection is suspected, rapid recognition and effective antibiotic treatment combined with surgery are critical to improving clinical outcomes. More attention to determination and reporting of C. perfringens toxins will provide new insights and could reveal new targets for intervention.

Table 1.

Summary of the laboratory data.

	10.30 (AM)	10.30 (PM)	10.31	11.1	11.2	11.10
WBC (×109/L)	9.82↑	9.97↑	19.11↑	14.61↑	12.7↑	8.55
NEU (%)	96↑	96↑	95↑	94↑	89.7↑	72.8
HGB (g/L)	156	135	138	133	133	153
PLT (×109/L)	117↓	89↓	77↓	75↓	86↓	268
h-CRP (mg/L)	11↑	17↑	100↑	50↑	60.2↑	2
K+ (mmol/L)	3.82	4.2	4.5	4.26	4.5	3.75
NA+ (mmol/L)	137	135	134	130	134	136
CL+ (mmol/L)	97.7	98.4	99.3	101	109	98.2
CREA (μmol/L)	76	89	81	70	64	62
UREA (mmol/L)	7.69↑	8.56↑	8.68↑	9.71↑	8.77↑	6.23
TBIL (μmol/L)	79.4↑	93.2↑	110.1↑	34.2↑	19	23.4
ALT (U/L)	178.6	195.7↑	127↑	84↑	44	50
AST (U/L)	637↑	734↑	216↑	83↑	30	47
GGT (U/L)	328↑	387↑	284.5↑	215↑	160↑	127↑

– increase;

– decrease.

Table 2.

Cases of C. perfringens bloodstream infection in humans with gastrointestinal disease.

Ref. No.	Year	Age	Sex	Pathologic diagnosis	Outcome	Country
12	2013	48	Female	Cholecystitis	Survival	USA
13	2020	80	Female	Gastrointestinal symptoms	Death	Japan
14	2010	75	Female	Cholecystitis	Death	USA
15	1989	69	Man	Gallbladder empyema	Survival	Japan
16	2010	74	Man	Cholangitis	Survival	Netherlands
17	1996	67	Male	Gallbladder stones	Survival	USA
18	2018	81	Male	Gastrointestinal stromal tumor	Death	Germany
19	2016	46	Male	Cholecystitis	Survival	Netherlands
20	2019	47	Male	Ulcerative colitis	Survival	Iran
21	2020	40	Female	Enterocolitis	Survival	Australian
22	2016	76	Female	Emphysematous gastritis	Death	Hungary
23	2012	21	Male	Necrotizing duodenitis	Survival	Japan
24	2011	60	Male	Cholecystitis	Death	USA
25	2015	81	Female	Gastroenteritis	Death	Australian
26	2020	63	Male	Gastroenteritis	Death	Switzerland
27	2007	73	Male	Cholecystitis	Death	Japan
28	2013	77	Male	Cholecystitis	Death	Finland
29	2000	79	Female	Colitis	Survival	China
30	2020	51	Male	Enteritis	Death	France
31	2005	83	Female	Cholecystitis	Survival	Japan

Table 3.

Cases of Clostridium perfringens co-infection pathogens.

Ref. No.	Year	Co-infection pathogen	Species	Age	Outcome
10	1993	E. coli	Human	18	Death
32	2018	C. sordellii	Human	80	Survival
33	2012	T. gondii	Human	19	Survival
34	2013	E. coli	Pig	NA	Death
35	2007	S. aureus & E. coli	Goat	NA	Death
36	2016	C. difficile	Dogs (2 cases)	NA	Death
37	2020	Klebsiella variicola	Human	68	Survival