Mohammad Ali Saghiri1, Nader Sheibani2, Toshihisa Kawai3, Devyani Nath4, Sahar Dadvand5, Saeid B Amini6, Julia Vakhnovetsky7, Steven M Morgano8. 1. Biomaterials Laboratory, Department of Restorative Dentistry, Rutgers School of Dental Medicine, NJ, USA; Department of Endodontics, University of the Pacific, Arthur A. Dugoni School of Dentistry, CA, USA. Electronic address: saghiri@gmail.com. 2. Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA. 3. College of Dental Medicine, Nova Southeastern University, FL, USA. 4. Lab Manager of Biomaterials Laboratory, Department of Restorative Dentistry, Rutgers School of Dental Medicine, NJ, USA. 5. Department of Preventive and Restorative Dental Sciences, San Francisco School of Dentistry, University of California, San Francisco, CA, USA. 6. Statistics Department, George Washington University, Washington, DC, USA. 7. Sector of Angiogenesis Regenerative Medicine, Dr. Hajar Afsar Lajevardi Research Cluster (DHAL), Hackensack, NJ, USA; Rutgers School of Dental Medicine, Newark, NJ, USA. 8. Department of Restorative Dentistry, Rutgers School of Dental Medicine, NJ, USA.
Abstract
OBJECTIVE: This study was performed to evaluate the effect of type 1 diabetes mellitus (T1DM) on the microhardness of tooth enamel and dentine in mice. DESIGN: Seventy male C57BL/6 J mice were used in this study. Thirty-five mice were rendered diabetic by administration of streptozotocin (STZ), and the remaining animals received citrate buffer (normal/non-diabetic). In each group, specimens were divided into 7 subgroups of 5 mice based on the time points 0, 1, 4, 8, 12, 20, and 28 weeks. The microhardness value (MHV) of the second molars' enamel and root dentine were tested with a Vickers microhardness tester. Five specimens from each subgroup were evaluated for dentinal tubular density by scanning electron microscope (SEM) and color dot map analysis to determine the color intensity of strontium (Sr) and magnesium (Mg) by using ImageJ software. RESULTS: The MHV of enamel was significantly reduced in STZ specimens in time points of 12 weeks (STZ: 274.39 ± 15.42, normal: 291.22 ± 15.28), 20 weeks (STZ: 247.28 ± 19.65, normal: 290.68 ± 11.52), and 28 weeks (STZ: 232.87 ± 15.07, normal: 282.76 ± 10.36) (P < 0.05). When comparing the MHV of dentine in subgroups of the normal group, after 20 weeks (169.1 ± 7.5) and 28 weeks (168.6 ± 7.81), the MHV increased significantly (P < 0.05). However, in the STZ group, a significant reduction of MHV was noticed between 28 weeks (131.69 ± 6.2) specimens with other subgroups (P < 0.05). CONCLUSIONS: T1DM negatively affected enamel and dentine microhardness, and enamel was influenced much more negatively and rapidly compared with dentine in diabetic groups.
OBJECTIVE: This study was performed to evaluate the effect of type 1 diabetes mellitus (T1DM) on the microhardness of tooth enamel and dentine in mice. DESIGN: Seventy male C57BL/6 J mice were used in this study. Thirty-five mice were rendered diabetic by administration of streptozotocin (STZ), and the remaining animals received citrate buffer (normal/non-diabetic). In each group, specimens were divided into 7 subgroups of 5 mice based on the time points 0, 1, 4, 8, 12, 20, and 28 weeks. The microhardness value (MHV) of the second molars' enamel and root dentine were tested with a Vickers microhardness tester. Five specimens from each subgroup were evaluated for dentinal tubular density by scanning electron microscope (SEM) and color dot map analysis to determine the color intensity of strontium (Sr) and magnesium (Mg) by using ImageJ software. RESULTS: The MHV of enamel was significantly reduced in STZ specimens in time points of 12 weeks (STZ: 274.39 ± 15.42, normal: 291.22 ± 15.28), 20 weeks (STZ: 247.28 ± 19.65, normal: 290.68 ± 11.52), and 28 weeks (STZ: 232.87 ± 15.07, normal: 282.76 ± 10.36) (P < 0.05). When comparing the MHV of dentine in subgroups of the normal group, after 20 weeks (169.1 ± 7.5) and 28 weeks (168.6 ± 7.81), the MHV increased significantly (P < 0.05). However, in the STZ group, a significant reduction of MHV was noticed between 28 weeks (131.69 ± 6.2) specimens with other subgroups (P < 0.05). CONCLUSIONS: T1DM negatively affected enamel and dentine microhardness, and enamel was influenced much more negatively and rapidly compared with dentine in diabetic groups.
Authors: Mohammad Ali Saghiri; Ali Mohammad Saghiri; Elham Samadi; Devyani Nath; Julia Vakhnovetsky; Steven M Morgano Journal: Odontology Date: 2022-07-10 Impact factor: 2.885