| Literature DB >> 35524495 |
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Abstract
Entities:
Year: 2022 PMID: 35524495 PMCID: PMC9077295 DOI: 10.1111/jcmm.17302
Source DB: PubMed Journal: J Cell Mol Med ISSN: 1582-1838 Impact factor: 5.310
FIGURE 5Knocking out MALT1 inhibits EGFR‐induced NF‐κB activation in GBM cells. (A and B) The effects of knocking out MALT1 on the levels of IκB‐α in U87 and U251 cells as assessed by western blot analysis. (C and D) Subcellular location of p65 was detected using cellular fractionation and immunoblotting after knocking out MALT1. Lamin A/C and actin were used as nuclear and cytoplasm loading controls, respectively. (E and F) Knocking out MALT1 inhibited EGF‐induced degradation of IκB‐α. The cells were then stimulated with EGF (100 ng/mL) for the indicated times. IκB‐α expression was analysed by western blot analysis. (G and H) MALT1 knockout suppressed EGF‐induced nuclear translocation of p65 in GBM cells. The cells were stimulated with EGF (100 ng/mL) for the indicated time, and then cell lysates were subjected to western blotting using p65 antibodies.