| Literature DB >> 35515 |
Abstract
We have partially characterized the biochemical parameters of glutamine synthetase from Klebsiella pneumoniae and have shown that the differential affinity of adenylylated and unadenylylated glutamine synthetase for adenosine diphosphate provides a convenient means of determining the adenylylation state. Using this assay procedure, we examined the relationship between the adenylylation state and the expression of other genes involved in nitrogen assimilation. We observed no correlation between the adenylylation state and the expression of histidase, glutamine synthetase, glutamate synthase, glutamate dehydrogenase, and urease in aerobic cultures.Entities:
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Year: 1979 PMID: 35515 PMCID: PMC218311 DOI: 10.1128/jb.137.3.1282-1289.1979
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490