Literature DB >> 35508993

Correction: A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples.

Kiran K Mangalaparthi1, Sandip Chavan1, Anil K Madugundu1,2,3,4, Santosh Renuse1,5, Patrick M Vanderboom1, Anthony D Maus1, Jennifer Kemp1, Benjamin R Kipp6, Stefan K Grebe1,7, Ravinder J Singh1, Akhilesh Pandey8,9,10.   

Abstract

Entities:  

Year:  2022        PMID: 35508993      PMCID: PMC9065230          DOI: 10.1186/s12014-022-09355-z

Source DB:  PubMed          Journal:  Clin Proteomics        ISSN: 1542-6416            Impact factor:   5.000


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Correction to: Clin Proteom (2021) 18:25 https://doi.org/10.1186/s12014-021-09331-z

The original version [1] of this article contains errors in Tables 2 and 3 as follows: In Table 2: Because of a formatting error, the mean values of three peptides in Set 2 were listed under Set 1 and the mean values for Set 3 were listed under Set 2. In Table 3: The values under “Positive” and “Negative” columns for the row describing “SARS-CoV-2 negative nasopharyngeal swab samples (n = 30)” were switched and the “Specificity” was incorrectly represented as “~ 100%” instead of “100%.” The authors regret these errors, which have now been corrected. The corrected Tables 2 and 3 are given.
Table 2

Variability (reported as CV) for SISCAPA assay performed on 3 separate sets of pooled RT-PCR positive nasopharyngeal swab samples (Ct value < 24)

DGIIWVATEGALNTPKITFGGPSDSTGSNQNGERNPANNAAIVLQLPQGTTLPK
Set 1
 Mean1.64E+084.90E+076.04E+06
 SD2.63E+073.37E+066.28E+05
 CV16.046.8810.38
Set 2
 Mean1.62E+085.15E+075.69E+06
 SD2.13E+074.30E+065.50E+05
 CV13.148.349.66
Set 3
 Mean1.87E+084.28E+076.27E+06
 SD1.06E+073.60E+063.90E+05
 CV5.648.416.21

The total area was considered for calculating the mean and standard deviation

Table 3

Summary of MS-based detection of SARS-CoV-2 viral peptides from clinical nasopharyngeal swab samples using the SISCAPA workflow

Detection of SARS-CoV-2 viral peptides by SISCAPA approach
PositiveNegative
SARS-CoV-2 positive nasopharyngeal swab samples (n = 41)383Sensitivity—92.68%
SARS-CoV-2 negative nasopharyngeal swab samples (n = 30)030Specificity—100%
Variability (reported as CV) for SISCAPA assay performed on 3 separate sets of pooled RT-PCR positive nasopharyngeal swab samples (Ct value < 24) The total area was considered for calculating the mean and standard deviation Summary of MS-based detection of SARS-CoV-2 viral peptides from clinical nasopharyngeal swab samples using the SISCAPA workflow
  1 in total

1.  A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples.

Authors:  Kiran K Mangalaparthi; Sandip Chavan; Anil K Madugundu; Santosh Renuse; Patrick M Vanderboom; Anthony D Maus; Jennifer Kemp; Benjamin R Kipp; Stefan K Grebe; Ravinder J Singh; Akhilesh Pandey
Journal:  Clin Proteomics       Date:  2021-10-22       Impact factor: 3.988

  1 in total

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