| Literature DB >> 35508993 |
Kiran K Mangalaparthi1, Sandip Chavan1, Anil K Madugundu1,2,3,4, Santosh Renuse1,5, Patrick M Vanderboom1, Anthony D Maus1, Jennifer Kemp1, Benjamin R Kipp6, Stefan K Grebe1,7, Ravinder J Singh1, Akhilesh Pandey8,9,10.
Abstract
Entities:
Year: 2022 PMID: 35508993 PMCID: PMC9065230 DOI: 10.1186/s12014-022-09355-z
Source DB: PubMed Journal: Clin Proteomics ISSN: 1542-6416 Impact factor: 5.000
Variability (reported as CV) for SISCAPA assay performed on 3 separate sets of pooled RT-PCR positive nasopharyngeal swab samples (Ct value < 24)
| DGIIWVATEGALNTPK | ITFGGPSDSTGSNQNGER | NPANNAAIVLQLPQGTTLPK | |
|---|---|---|---|
| Set 1 | |||
| Mean | 1.64E+08 | 4.90E+07 | 6.04E+06 |
| SD | 2.63E+07 | 3.37E+06 | 6.28E+05 |
| CV | 16.04 | 6.88 | 10.38 |
| Set 2 | |||
| Mean | 1.62E+08 | 5.15E+07 | 5.69E+06 |
| SD | 2.13E+07 | 4.30E+06 | 5.50E+05 |
| CV | 13.14 | 8.34 | 9.66 |
| Set 3 | |||
| Mean | 1.87E+08 | 4.28E+07 | 6.27E+06 |
| SD | 1.06E+07 | 3.60E+06 | 3.90E+05 |
| CV | 5.64 | 8.41 | 6.21 |
The total area was considered for calculating the mean and standard deviation
Summary of MS-based detection of SARS-CoV-2 viral peptides from clinical nasopharyngeal swab samples using the SISCAPA workflow
| Detection of SARS-CoV-2 viral peptides by SISCAPA approach | |||
|---|---|---|---|
| Positive | Negative | ||
| SARS-CoV-2 positive nasopharyngeal swab samples (n = 41) | 38 | 3 | Sensitivity—92.68% |
| SARS-CoV-2 negative nasopharyngeal swab samples (n = 30) | 0 | 30 | Specificity—100% |