Literature DB >> 35507176

Application of 3D Culture Assays to Study Breast Morphogenesis, Epithelial Plasticity, and Cellular Interactions in an Epithelial Progenitor Cell Line.

Anna Karen Sigurdardottir1, Bylgja Hilmarsdottir2, Thorarinn Gudjonsson1,3, Gunnhildur Asta Traustadottir4.   

Abstract

Capturing breast morphogenesis and cancer progression in 3D culture using cell lines with stem cell properties can greatly increase understanding of the underlying mechanisms involved in these processes, highlighting the importance of the culture method. D492 is a breast epithelial progenitor cell line that provides a model for branching morphogenesis when cultured in 3D reconstituted basement membrane matrix (rBM). Along with its derivate cell lines D492M and D492HER2, D492 also serves as a robust model for epithelial to mesenchymal transition (EMT) and tumorigenicity, respectively. Here, we describe the routine maintenance and application of the D492 cell lines in 3D culture for the study of branching morphogenesis, EMT and epithelial-endothelial interaction.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  3D culture; Branching morphogenesis; Breast endothelial cells (BRENCs); D492 cell lines; Matrigel®

Mesh：

Year:  2022        PMID： 35507176     DOI： 10.1007/978-1-0716-1979-7_26

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties.

Authors:  Thorarinn Gudjonsson; René Villadsen; Helga Lind Nielsen; Lone Rønnov-Jessen; Mina J Bissell; Ole William Petersen
Journal:  Genes Dev       Date:  2002-03-15       Impact factor: 11.361

Review 2.  Microenvironmental regulation of tumor progression and metastasis.

Authors:  Daniela F Quail; Johanna A Joyce
Journal:  Nat Med       Date:  2013-11       Impact factor: 53.440

Review 3.  Keeping abreast of the mammary epithelial hierarchy and breast tumorigenesis.

Authors:  Jane E Visvader
Journal:  Genes Dev       Date:  2009-11-15       Impact factor: 11.361

Review 4.  Mammary gland development.

Authors:  Hector Macias; Lindsay Hinck
Journal:  Wiley Interdiscip Rev Dev Biol       Date:  2012 Jul-Aug       Impact factor: 5.814

Review 5.  Application of the D492 Cell Lines to Explore Breast Morphogenesis, EMT and Cancer Progression in 3D Culture.

Authors:  Eirikur Briem; Saevar Ingthorsson; Gunnhildur Asta Traustadottir; Bylgja Hilmarsdottir; Thorarinn Gudjonsson
Journal:  J Mammary Gland Biol Neoplasia       Date:  2019-01-25       Impact factor: 2.673

6.  ECM1 secreted by HER2-overexpressing breast cancer cells promotes formation of a vascular niche accelerating cancer cell migration and invasion.

Authors:  Sophie Sarah Steinhaeuser; Erika Morera; Zuzana Budkova; Alexander Schepsky; Qiong Wang; Ottar Rolfsson; Angela Riedel; Aileen Krueger; Bylgja Hilmarsdottir; Gunhild Mari Maelandsmo; Bryndis Valdimarsdottir; Anna Karen Sigurdardottir; Bjarni Agnar Agnarsson; Jon Gunnlaugur Jonasson; Saevar Ingthorsson; Gunnhildur Asta Traustadottir; Thordur Oskarsson; Thorarinn Gudjonsson
Journal:  Lab Invest       Date:  2020-03-18       Impact factor: 5.662

7.  Endothelial induced EMT in breast epithelial cells with stem cell properties.

Authors:  Valgardur Sigurdsson; Bylgja Hilmarsdottir; Hekla Sigmundsdottir; Agla J R Fridriksdottir; Markus Ringnér; Rene Villadsen; Ake Borg; Bjarni A Agnarsson; Ole William Petersen; Magnus K Magnusson; Thorarinn Gudjonsson
Journal:  PLoS One       Date:  2011-09-06       Impact factor: 3.240

8.  HER2 induced EMT and tumorigenicity in breast epithelial progenitor cells is inhibited by coexpression of EGFR.

Authors:  S Ingthorsson; K Andersen; B Hilmarsdottir; G M Maelandsmo; M K Magnusson; T Gudjonsson
Journal:  Oncogene       Date:  2015-12-21       Impact factor: 9.867

9.  Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma.

Authors:  Lucas A Tomko; Ryan C Hill; Alexander Barrett; Joseph M Szulczewski; Matthew W Conklin; Kevin W Eliceiri; Patricia J Keely; Kirk C Hansen; Suzanne M Ponik
Journal:  Sci Rep       Date:  2018-08-28       Impact factor: 4.379

10.  YKL-40/CHI3L1 facilitates migration and invasion in HER2 overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation.

Authors:  Erika Morera; Sarah Sophie Steinhäuser; Zuzana Budkova; Saevar Ingthorsson; Jennifer Kricker; Aileen Krueger; Gunnhildur Asta Traustadottir; Thorarinn Gudjonsson
Journal:  In Vitro Cell Dev Biol Anim       Date:  2019-09-03       Impact factor: 2.416
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