| Literature DB >> 35505042 |
Enzo M Scutigliani1, Jakub A Kochan1,2, Emilie C B Desclos1, Art Jonker1, Michal Heger3,4, Przemek M Krawczyk5.
Abstract
Liposomal nanocarriers are intensively investigated as delivery vehicles for photoactivatable agents used in photodynamic therapy (PDT). The uptake, intracellular distribution, and processing of the nanocarriers are of paramount importance for the effectiveness of the therapy; visualization and analysis of these processes can, therefore, stimulate the development of improved PDT modalities. Here we describe a simple protocol, based on super-resolution imaging, that can be used for detailed quantification of concentration, distribution, and size of individual lipid nanocarriers in adherent mammalian cells.Entities:
Keywords: Photodynamic therapy; Photosensitizers; Super-resolution microscopy
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Year: 2022 PMID： 35505042 DOI： 10.1007/978-1-0716-2099-1_37
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745