Literature DB >> 3550389

Spot-transformation with plasmids.

W Vogel, H Schmieger.   

Abstract

A method is described which allows transformation of bacterial cells on the surface of agar plates. It is suitable for transferring large numbers of different plasmids, such as gene libraries, into a new genetic background. One nanogram of plasmid DNA is sufficient for transformation.

Mesh：

Year:  1986        PMID： 3550389     DOI： 10.1007/BF00338099

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  6 in total

1.  Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

Authors:  J G Sutcliffe
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

2.  Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.

Authors:  E M Lederberg; S N Cohen
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

3.  Salmonella typhimurium LT2 strains which are r- m+ for all three chromosomally located systems of DNA restriction and modification.

Authors:  L R Bullas; J I Ryu
Journal:  J Bacteriol       Date:  1983-10       Impact factor: 3.490

4.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

5.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

6.  Selective transduction of recombinant plasmids with cloned pac sites by Salmonella phage P22.

Authors:  C Schmidt; H Schmieger
Journal:  Mol Gen Genet       Date:  1984
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.