| Literature DB >> 35501640 |
E A Klimov1,2, V V Sobolev2,3, N A Batashkov1, Z G Kokaeva1, A V Tretiakov1, Z A Nevozinskaya4,5, I M Korsunskaya6,7, T N Sukhova5, O E Evina8, A Z Khashukoeva5.
Abstract
Lichen sclerosus of the vulva is a common, but poorly studied disease. We assessed the level of transcriptional activity of APAF1, BAX, BCL2, BIRC5, CCND1, DAPK1, MCL1, and MYC genes encoding products that control apoptosis in the samples of tissues affected by vulvar lichen sclerosus and adjacent control tissues (n=24). Analysis of transcriptional activity was performed by real-time PCR using specific primers and SYBR Green intercalating dye. After the total group was divided by the presence of the concomitant gynecological diseases, a significant increase in the transcriptional activity of the CCND1 gene was revealed in patients with concomitant uterine fibroids. This may indicate the possible role of the activation of mitosis during tumor initiation.Entities:
Keywords: apoptosis; polymerase chain reaction; transcriptional gene activity; uterine fibroid; vulvar lichen sclerosus
Mesh：
Year: 2022 PMID： 35501640 DOI： 10.1007/s10517-022-05467-6
Source DB: PubMed Journal: Bull Exp Biol Med ISSN： 0007-4888 Impact factor: 0.804