Literature DB >> 3549453

Expression of synthetic suppressor tRNA genes under the control of a synthetic promoter.

J M Masson, J H Miller.   

Abstract

A synthetic promoter derived from the Escherichia coli lipoprotein promoter sequence was used to express synthetic suppressor tRNA genes in E. coli. These constructs, cloned into a plasmid of the pEMBL family, gave very high yields of suppressor tRNA in vivo.

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Year:  1986        PMID： 3549453     DOI： 10.1016/0378-1119(86)90061-2

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  26 in total

1.  Importance of the reverse Hoogsteen base pair 54-58 for tRNA function.

Authors:  Ekaterina I Zagryadskaya; Felix R Doyon; Sergey V Steinberg
Journal:  Nucleic Acids Res       Date:  2003-07-15       Impact factor: 16.971

2.  Structure-function analysis of tRNA(Gln) in an Escherichia coli knockout strain.

Authors:  William H McClain; Kay Gabriel; Dennis Lee; Sharee Otten
Journal:  RNA       Date:  2004-05       Impact factor: 4.942

Review 3.  High-expression of a target gene and high-stability of the plasmid.

Authors:  M Kobayashi; Y Kurusu; H Yukawa
Journal:  Appl Biochem Biotechnol       Date:  1991-02       Impact factor: 2.926

4.  A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold.

Authors:  Luc Ponchon; Geneviève Beauvais; Sylvie Nonin-Lecomte; Frédéric Dardel
Journal:  Nat Protoc       Date:  2009-05-28       Impact factor: 13.491

5.  Genes for 7S RNAs can replace the gene for 4.5S RNA in growth of Escherichia coli.

Authors:  S Brown
Journal:  J Bacteriol       Date:  1991-03       Impact factor: 3.490

6.  Amber suppression in Escherichia coli by unusual mitochondria-like transfer RNAs.

Authors:  V Bourdeau; S V Steinberg; G Ferbeyre; R Emond; N Cermakian; R Cedergren
Journal:  Proc Natl Acad Sci U S A       Date:  1998-02-17       Impact factor: 11.205

7.  Recognition of acceptor-stem structure of tRNA(Asp) by Escherichia coli aspartyl-tRNA synthetase.

Authors:  Hyunsic Choi; Kay Gabriel; Jay Schneider; Sharee Otten; William H McClain
Journal:  RNA       Date:  2003-04       Impact factor: 4.942

8.  Aptamer redesigned tRNA is nonfunctional and degraded in cells.

Authors:  Dennis Lee; William H McClain
Journal:  RNA       Date:  2004-01       Impact factor: 4.942

9.  Genetic selection and DNA sequences of 4.5S RNA homologs.

Authors:  S Brown; G Thon; E Tolentino
Journal:  J Bacteriol       Date:  1989-12       Impact factor: 3.490

10.  tRNA nucleotide 47: an evolutionary enigma.

Authors:  N Cermakian; W H McClain; R Cedergren
Journal:  RNA       Date:  1998-08       Impact factor: 4.942
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