Literature DB >> 3549057

Enzymic ethanol assay: a new colorimetric method based on measurement of hydrogen peroxide.

L Prencipe, E Iaccheri, C Manzati.   

Abstract

We describe a new colorimetric method for measuring ethanol in plasma by use of a peroxidase-coupled assay system and alcohol oxidase (EC 1.1.3.13) from Pichia species. Absorptivity is low enough to give useful results without sample dilution. The procedure is also applicable to saliva samples and utilizes only one working reagent. The absorbance of the blue dye that is formed is measured at 600 nm. The standard curve for the method is linear for ethanol concentrations up to 4 g/L. Average analytical recovery of ethanol in human plasma exceeded 99%. Within-run and between-run CVs were less than 2.45% and less than 1.92%, respectively. Results correlate very well with those by gas chromatography (r = 0.9977). The method is adaptable to automation.

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Year:  1987        PMID： 3549057

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  9 in total

1.  Ketamine and MAG Lipase Inhibitor-Dependent Reversal of Evolving Depressive-Like Behavior During Forced Abstinence From Alcohol Drinking.

Authors:  Katherine M Holleran; Hadley H Wilson; Tracy L Fetterly; Rebecca J Bluett; Samuel W Centanni; Rachel A Gilfarb; Lauren E R Rocco; Sachin Patel; Danny G Winder
Journal:  Neuropsychopharmacology       Date:  2016-01-11       Impact factor: 7.853

2.  Tolerance to cannabinoid-induced behaviors in mice treated chronically with ethanol.

Authors:  Matthew J Pava; Emily M Blake; Stephen T Green; Brandon J Mizroch; Patrick J Mulholland; John J Woodward
Journal:  Psychopharmacology (Berl)       Date:  2011-06-24       Impact factor: 4.530

3.  Noradrenergic tone mediates marble burying behavior after chronic stress and ethanol.

Authors:  Carolina R den Hartog; Katrina L Blandino; McKenzie L Nash; Emily R Sjogren; Michael A Grampetro; David E Moorman; Elena M Vazey
Journal:  Psychopharmacology (Berl)       Date:  2020-06-25       Impact factor: 4.530

4.  Deficits in the extinction of ethanol-seeking behavior following chronic intermittent ethanol exposure are attenuated with positive allosteric modulation of mGlu5.

Authors:  J T Gass; J T McGonigal; L J Chandler
Journal:  Neuropharmacology       Date:  2016-10-08       Impact factor: 5.250

5.  Alcohol ingestion lowers supine blood pressure, causes splanchnic vasodilatation and worsens postural hypotension in primary autonomic failure.

Authors:  K R Chaudhuri; S Maule; T Thomaides; D Pavitt; C J Mathias
Journal:  J Neurol       Date:  1994-01       Impact factor: 4.849

6.  Chronic alcohol disrupts dopamine receptor activity and the cognitive function of the medial prefrontal cortex.

Authors:  Heather Trantham-Davidson; Elizabeth J Burnett; Justin T Gass; Marcelo F Lopez; Patrick J Mulholland; Samuel W Centanni; Stan B Floresco; L Judson Chandler
Journal:  J Neurosci       Date:  2014-03-05       Impact factor: 6.167

7.  MPEP Lowers Binge Drinking in Male and Female C57BL/6 Mice: Relationship with mGlu5/Homer2/Erk2 Signaling.

Authors:  Gan Huang; Summer L Thompson; Jane R Taylor
Journal:  Alcohol Clin Exp Res       Date:  2021-03-28       Impact factor: 3.455

8.  Homer2 deletion alters dendritic spine morphology but not alcohol-associated adaptations in GluN2B-containing N-methyl-D-aspartate receptors in the nucleus accumbens.

Authors:  Natalie S McGuier; Audrey E Padula; Patrick J Mulholland; L Judson Chandler
Journal:  Front Pharmacol       Date:  2015-02-23       Impact factor: 5.810

9.  Impact of sex, strain, and age on blood ethanol concentration and behavioral signs of intoxication during ethanol vapor exposure.

Authors:  Elizabeth J Glover; Fauzan Khan; Kacey Clayton-Stiglbauer; L Judson Chandler
Journal:  Neuropharmacology       Date:  2020-11-19       Impact factor: 5.250
  9 in total

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