| Literature DB >> 35488296 |
Xiao Cen1,2, Xuefeng Pan1,3, Bo Zhang1,3, Wei Huang1,3, Fang Pei1,3, Tao Luo4, Xinqi Huang5,6, Jun Liu1,3, Zhihe Zhao1,3.
Abstract
Entities:
Year: 2022 PMID: 35488296 PMCID: PMC9055762 DOI: 10.1186/s13287-022-02870-8
Source DB: PubMed Journal: Stem Cell Res Ther ISSN: 1757-6512 Impact factor: 6.832
Fig. 1miR-20a-5p was up-regulated during the osteogenesis of hDPSCs. A hDPSCs were positive for CD29 and CD44, while negative for CD34 and CD45. B The morphology of primary hDPSCs was spindle-shaped fibroblast-like. C–E Osteogenic and chondrogenic differentiations were evaluated by ARS and Alcian Blue staining respectively, and Oil Red O staining was performed for testing adipogenic differentiation. F ALP and ARS staining after osteogenic induction for 1, 7, and 14 days. G RUNX2 and OPN mRNA levels after osteogenic induction for 1, 7, and 14 days. H The expression levels of miR-20a-5p from day 1 to day 14. *p < 0.05 and **p < 0.01 compared with osteogenic induction for 1 day