Literature DB >> 35486252

Grafting of Epiblast Stem Cell into the Epiblast and Whole-Embryo Imaging to Unveil Lineage Competence.

Pierre Osteil1,2, Joshua B Studdert3,4, Patrick P L Tam3,5.   

Abstract

Here we describe a method to engraft epiblast stem cells (EpiSC) into the epiblast of gastrulation-stage mouse embryo to test the lineage propensity acquired by the EpiSCs during in vitro culture under different signaling conditions. After dissection and grafting, the recipient embryos can be grown in whole-embryo culture for up to 48 h and the contribution of the EpiSC-derived cells to tissues in the recipient embryo is assessed by light sheet 3D microscopy.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  EpiSC; Epiblast; Grafting; Light sheet microscopy

Mesh：

Year:  2022        PMID： 35486252     DOI： 10.1007/978-1-0716-2281-0_19

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

1.  Culture of whole mouse embryos at early postimplantation to organogenesis stages: developmental staging and methods.

Authors:  Jaime A Rivera-Pérez; Vanessa Jones; Patrick P L Tam
Journal:  Methods Enzymol       Date:  2010       Impact factor: 1.600

2.  Inhibition of Wnt/β-catenin signaling by IWR1 induces expression of Foxd3 to promote mouse epiblast stem cell self-renewal.

Authors:  Kuisheng Liu; Yuanyuan Sun; Dahai Liu; Shoudong Ye
Journal:  Biochem Biophys Res Commun       Date:  2017-06-16       Impact factor: 3.575

3.  Anterior primitive endoderm may be responsible for patterning the anterior neural plate in the mouse embryo.

Authors:  P Thomas; R Beddington
Journal:  Curr Biol       Date:  1996-11-01       Impact factor: 10.834

Review 4.  Postimplantation mouse development: whole embryo culture and micro-manipulation.

Authors:  P P Tam
Journal:  Int J Dev Biol       Date:  1998       Impact factor: 2.203

5.  New cell lines from mouse epiblast share defining features with human embryonic stem cells.

Authors:  Paul J Tesar; Josh G Chenoweth; Frances A Brook; Timothy J Davies; Edward P Evans; David L Mack; Richard L Gardner; Ronald D G McKay
Journal:  Nature       Date:  2007-06-27       Impact factor: 49.962

6.  The transcriptional and functional properties of mouse epiblast stem cells resemble the anterior primitive streak.

Authors:  Yoji Kojima; Keren Kaufman-Francis; Joshua B Studdert; Kirsten A Steiner; Melinda D Power; David A F Loebel; Vanessa Jones; Angelyn Hor; Gustavo de Alencastro; Grant J Logan; Erdahl T Teber; Oliver H Tam; Michael D Stutz; Ian E Alexander; Hilda A Pickett; Patrick P L Tam
Journal:  Cell Stem Cell       Date:  2013-10-17       Impact factor: 24.633

7.  An alternative pluripotent state confers interspecies chimaeric competency.

Authors:  Jun Wu; Daiji Okamura; Mo Li; Keiichiro Suzuki; Chongyuan Luo; Li Ma; Yupeng He; Zhongwei Li; Chris Benner; Isao Tamura; Marie N Krause; Joseph R Nery; Tingting Du; Zhuzhu Zhang; Tomoaki Hishida; Yuta Takahashi; Emi Aizawa; Na Young Kim; Jeronimo Lajara; Pedro Guillen; Josep M Campistol; Concepcion Rodriguez Esteban; Pablo J Ross; Alan Saghatelian; Bing Ren; Joseph R Ecker; Juan Carlos Izpisua Belmonte
Journal:  Nature       Date:  2015-05-06       Impact factor: 49.962

8.  Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells.

Authors:  Tomoyuki Sumi; Shinya Oki; Keiko Kitajima; Chikara Meno
Journal:  PLoS One       Date:  2013-05-14       Impact factor: 3.240

9.  Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.

Authors:  Anestis Tsakiridis; Yali Huang; Guillaume Blin; Stavroula Skylaki; Filip Wymeersch; Rodrigo Osorno; Costas Economou; Eleni Karagianni; Suling Zhao; Sally Lowell; Valerie Wilson
Journal:  Development       Date:  2014-03       Impact factor: 6.868

10.  Derivation of pluripotent epiblast stem cells from mammalian embryos.

Authors:  I Gabrielle M Brons; Lucy E Smithers; Matthew W B Trotter; Peter Rugg-Gunn; Bowen Sun; Susana M Chuva de Sousa Lopes; Sarah K Howlett; Amanda Clarkson; Lars Ahrlund-Richter; Roger A Pedersen; Ludovic Vallier
Journal:  Nature       Date:  2007-06-27       Impact factor: 49.962
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