Literature DB >> 35482183

Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation.

Byong H Kang1,2, Brianna M Lax3,2, K Dane Wittrup4,5,6.   

Abstract

Yeast surface display is a powerful directed evolution method for developing and engineering protein molecules to attain desired properties. Here, updated protocols are presented for purposes of identification of lead binders and their affinity maturation. Large libraries are screened by magnetic bead selections followed by flow cytometric selections. Upon identification and characterization of single clones, their affinities are improved by an iterative process of mutagenesis and fluorescence-activated cell sorting.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Affinity maturation; Directed evolution; Library screening; Protein engineering; Yeast surface display

Mesh：

Year:  2022        PMID： 35482183     DOI： 10.1007/978-1-0716-2285-8_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  83 in total

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Journal:  FEBS Lett       Date:  1999-08-27       Impact factor: 4.124

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Authors:  R W Roberts; J W Szostak
Journal:  Proc Natl Acad Sci U S A       Date:  1997-11-11       Impact factor: 11.205

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Journal:  Nat Biotechnol       Date:  1997-01       Impact factor: 54.908

5.  In vitro virus: bonding of mRNA bearing puromycin at the 3'-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro.

Authors:  N Nemoto; E Miyamoto-Sato; Y Husimi; H Yanagawa
Journal:  FEBS Lett       Date:  1997-09-08       Impact factor: 4.124

6.  In vitro selection and evolution of functional proteins by using ribosome display.

Authors:  J Hanes; A Plückthun
Journal:  Proc Natl Acad Sci U S A       Date:  1997-05-13       Impact factor: 11.205

7.  Yeast surface display for screening combinatorial polypeptide libraries.

Authors:  E T Boder; K D Wittrup
Journal:  Nat Biotechnol       Date:  1997-06       Impact factor: 54.908

Review 8.  Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systems.

Authors:  Jeffrey L Brodsky; William R Skach
Journal:  Curr Opin Cell Biol       Date:  2011-06-12       Impact factor: 8.382

9.  Isolation of anti-CD22 Fv with high affinity by Fv display on human cells.

Authors:  Mitchell Ho; Satoshi Nagata; Ira Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  2006-06-08       Impact factor: 11.205

10.  Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface.

Authors:  G P Smith
Journal:  Science       Date:  1985-06-14       Impact factor: 47.728
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