The Heck reaction of allylic alcohols catalysed by an N-heterocyclic carbene-Pd(ii) complex and toxicity of the ligand precursor for the marine benthic copepod Amphiascoides atopus.

The palladium-catalysed reaction of aryl halides and allylic alcohols is an attractive method for obtaining α,β-unsaturated aldehydes and ketones, which represent key intermediates in organic synthesis. In this context, a 1,2,3-triazol-5-ylidene (aNHC)-based palladium(ii) complex formed in situ has been found to be a selective catalyst for the syntheses of building blocks from the corresponding aryl halides and allylic alcohols, with yields ranging from 50% to 90%. The lack of toxic effects of the ligand precursor (1,2,3-triazolium salt) of the palladium(ii) complex for the harpacticoid copepod Amphiascoides atopus allowed us to contrast the efficiency of the catalytic system with the potential impact of the principal waste chemical in global aquatic ecosystems, which has not been previously addressed. This journal is © The Royal Society of Chemistry.

Introduction

Easy access to 1,2,3-triazoles via a simple copper-catalysed azide–alkyne cycloaddition reaction (CuAAC) has led to the development of a “post-click chemistry” strategy to obtain 1,2,3-triazolium salts by selective alkylation at the N3 position of the triazole ring.[1] 1,2,3-Triazolium salts represent an attractive group of chemical compounds given that they can be used as ionic liquids (ILs),[2] as hosts in anion recognition, as components of molecular machines and supramolecular assemblies[3] or as precursors of abnormal (mesoionic)[4] N-heterocyclic carbenes (aNHCs) as a consequence of their high stability.[5]

As shown in Scheme 1, deprotonation and metalation of 1,2,3-triazolium salts give a 1,2,3-triazol-5-ylidene complex (aNHC), which may be a precursor for an efficient catalyst for organic reactions thanks to its unique electronic features and donor properties.[6] Recently, 1,2,3-triazolium iodide salts have been used as efficient precursors for 1,2,3-triazol-5-ylidene ligands[7] (aNHCs) for palladium-catalysed Suzuki–Miyaaura[8] and Heck–Mizoroki cross-coupling reactions.[9] In those cases, the aNHC and palladium(ii) complex are formed in situ from triazolium salts under mild reaction conditions. With these relevant protocols, we focused on the use of a similar system in the synthesis of α–β unsaturated aldehydes and ketones via the Heck coupling of aryl halides and allylic alcohols, with the idea of developing an efficient and selective protocol using 1,2,3-triazol-5-ylidene (aNHC)-based palladium(ii) complexes.

Scheme 1

Synthesis and uses of 1,2,3-triazolium salt.

The high thermal stability and low vapour pressure make 1,2,3-triazolium salts attractive for industrial eco-friendly processes. It is assumed that their structural characteristics do not cause air pollution or damage to occupational health. However, their properties, such as resistance to photodegradation, water solubility and stability, suggest that they may be a threat to aquatic ecosystems due to bioaccumulation.[10] For that reason, we considered it necessary to compare the efficiency of the catalytic system (aNHC-based palladium(ii) complexes) with the impact of the 1,2,3-triazolium salt precursor in aquatic ecosystems.[11] Thus, we evaluated its acute toxicity in the harpacticoid copepod Amphiascoides atopus, considering that it will be among the principal waste chemicals eliminated after the catalytic process.

Sediments in aquatic ecosystems are rich in small crustaceans, including benthic invertebrates, which are effective indicators of impacts at higher levels of biological organization given their importance to overall ecosystem structure and function.[12] In particular, marine copepods belonging to the order Harpacticoida, one of the most abundant benthic invertebrates and an important food source for macroinvertebrates and fish, are suitable for use in tests that rapidly assess the acute, sublethal, or chronic effects of contaminants.[13] To our knowledge, there are no previous studies testing the toxicity of 1,2,3-triazolium salts in copepods or in other crustaceans.

Results and discussion

Catalytic activity

Initially, we carried out the synthesis of 1,2,3-triazolium iodide salts 4a and 4b with a simple copper-catalysed azide–alkyne cycloaddition (CuAAC) of alkyne 1, bromide derivatives 2a and 2b, and NaN3 under heterogeneous catalytic conditions. Then, direct methylation of both nitrogen atoms of the triazole and pyridine ring allowed us to obtain 1,3,4-trisubstituted-1,2,3-triazolium iodide salts 4a and 4b (Scheme 2).

Scheme 2

Synthesis of 1,3,4-trisubstituted-1,2,3-triazolium iodide salts 4a and 4b.

Then, we were interested in evaluating the catalytic properties of aNHC-based palladium(ii) complexes formed in situ from 4a and 4b in the Heck reaction between bromo pyridine 5a and allylic alcohol 6a to give ketone 7a. After various solvent systems were examined, we concluded that DMF was the best solvent (Table 1, entries 1–6). A higher reaction yield was obtained in the presence of Pd(OAc)2 and NaOAc (Table 1, entry 1). Furthermore, the yield was lowered when the reaction was carried out in the presence of PdCl2 (Table 1, entries 2 and 4) or when t-BuOK or K2CO3 was used as the base (Table 1, entries 7 and 8). When the reaction was performed in THF under reflux, only the starting material was observed in the 1H NMR spectrum of the reaction mixture (Table 1, entry 5). Product 7a was obtained in 55% and 50% yields at 50 °C (Table 1, entry 9), and it was not observed at room temperature (Table 1, entry 10) in the presence of 4a or 4b. When the reaction was carried out with 5 mol% 4a or 4b and 5 mol% Pd(OAc)2, the yield did not increase considerably (Table 1, entry 11). Notably, a complex reaction mixture was obtained in the absence of triazolium salts 4a or 4b.

Coupling of aryl halide 5a with allylic alcohol 6b in the presence of 4a or 4ba

Entry	Solvent	Pd(ii) source	Base	T (°C)	Yieldb (%)
1	DMF	Pd(OAc)2	NaOAc	90	80c and 78d
2	DMF	PdCl2	NaOAc	90	65c and 60d
3	Toluene	Pd(OAc)2	NaOAc	90	67c and 60d
4	Toluene	PdCl2	NaOAc	90	48c and 45d
5	THF	Pd(OAc)2	NaOAc	Reflux	0c and 0d
6	H2O	Pd(OAc)2	NaOAc	90	15c and 15d
7	DMF	Pd(OAc)2	t-BuOK	90	70c and 60d
8	DMF	Pd(OAc)2	K2CO3	90	72c and 62d
9	DMF	Pd(OAc)2	NaOAc	50	55c and 50d
10	DMF	Pd(OAc)2	NaOAc	rt	0c and 0d
11e	DMF	Pd(OAc)2	NaOAc	90	82c and 80d

Reaction conditions: aryl halide 5a (1.0 mmol), allylic alcohol 6a (1.0 mmol), base (2.0 mmol), Pd(ii) source (1 mol%), 1,2,3-triazolium salt 4 (1 mol%) and solvent (5 mL).

Yields of isolated product after chromatographic purification.

Yield obtained with 4a as aNHC precursor.

Yield obtained with 4b as aNHC precursor.

5% of Pd(OAc)2 and 5% of 1,2,3-triazolium salt 4 were used.

Using the best reaction conditions, we next examined the application of a 1,2,3-triazolium iodide and palladium salt system from the 4a aNHC precursor for the cross-couplings of a variety of aryl bromides with primary or secondary allylic alcohols (Table 2). The results indicate that the aNHC-based palladium(ii) complex formed in situ constituted an efficient and selective catalyst system for the synthesis of saturated aldehydes 7b–7e and ketones 7f–7r in good yields. In the case of tertiary allylic alcohols, the only final products were α–β unsaturated alcohols (7s–7v, Table 2). Unfortunately, the reaction does not evolve successfully when it was carried with aryl chlorides. One of the principal drawbacks of the reaction studied here is the formation of mixtures with both saturated and unsaturated carbonyl compounds as principal products,[14,15] which was not observed under our reaction conditions. Saturated carbonyl compounds were obtained as the only product, and unsaturated products (α–β unsaturated carbonyl compounds) were not observed when crude products were analysed by NMR.

Scope of N-heterocyclic carbene and Pd(ii) complex-catalysed allylic alcohol Heck reactiona,b

Reaction conditions: aryl halide 5 (1.0 mmol), allylic alcohol 6 (1.0 mmol), NaOAc (2.0 mmol), Pd(OAc)2 (1 mol%), and 1,2,3-triazolium salt 4a (1 mol%) and DMF (5 mL).

Yields of isolated product after chromatographic purification.

Regarding the reaction mechanism, we propose the direct metalation of 1,2,3-triazolium salt 4a with Pd(AcO)2 in the presence of NaOAc to obtain in situ 1,2,3-triazol-5-ylidene (aNHC)-based palladium(ii) complexes 8 (Scheme 3a), which should be the principal precursor to the catalytic species in the reaction. The catalytic process starts with the oxidative addition of bromo pyridine 5a, followed by coordination and regioselective alkene insertion (allylic alcohol 6a). Finally, Pd–H β-elimination involving carbinol hydrogen furnished desired carbonyl compound 7a.[16]

Scheme 3

(a) Proposed structure of 1,2,3-triazol-5-ylidene (aNHC)-based palladium(ii) complex 8. (b) Proposed mechanism for synthesis of α–β unsaturated aldehydes and ketones via the Heck coupling of aryl halide 5a and allylic alcohol 6a.

Toxicity of 1,2,3-triazolium salt 4a

Then, we used the harpacticoid copepod Amphiascoides atopus as a model to evaluate the toxicity (LC50 at 24, 48, 72 and 96 h) of 1,2,3-triazolium salt 4a. The results obtained were based on the mortality of adult copepods, which varied significantly among triazolium salt concentrations at every exposure time (Fig. 1, ANOVA: F = 18.156 for 24 h, F = 39.54 for 48 h, F = 45.562 for 72 h, and F = 34.951 for 96 h; all P < 0.0001). There was practically no mortality in unexposed (control) organisms and those exposed to 30 mg L−14a. Some mortality was observed at higher concentrations (Fig. 1); however, compared to the control, significant differences occurred above 250 mg L−1 at 24 h, 200 mg L−1 at 48 h, 150 mg L−1 at 72 h, and 100 mg L−1 at 96 h (all P < 0.05). The estimated LC50 values were 250.4 mg L−1 (CI 215.4 to 290.5 mg L−1) at 24 h, 173.9 mg L−1 (CI 150.4 to 196.4 mg L−1) at 48 h, 155 mg L−1 (CI 135.6 to 173.9 mg L−1) at 72 h, and 111.5 mg L−1 (CI 93.4 to 129.8 mg L−1) at 96 h. From these results and following the hazard classification of the US EPA adopted by Tsarpali and Dailianis[17] for crustaceans, the triazolium salt tested in the present study may be considered practically nontoxic since the estimated LC50 values were >100 mg L−1 at every exposure time. Similarly, Rodriguez Castillo et al.[18] reported no toxicity of [BMTriaz][NTf2] and [CF3CF2BTriaz][NTf2] triazolium salts against zebrafish Danio rerio.

Fig. 1

Toxicity of 1,2,3-triazolium salt 4a against (a) the harpacticoid copepod Amphiascoides atopus at (b) 24 h, (c) 48 h, (d) 72 h and (e) 96 h. Asterisk (*) indicates significant difference from controls.

The lack of toxic effect of triazolium salt 4a tested herein could be attributed to its hydrophilic properties, which limit its biocompatibility and adsorption onto or intercalation into the cellular membrane.[19] Our results are based only on the mortality of adult copepods. This may be a shortcoming considering that early developmental stages of copepods as well as sublethal endpoints are more sensitive indicators of the toxicity of contaminants.[20] Additionally, further studies are required to test the effect of the triazolium salt used in the present work on other aquatic organisms, including marine and freshwater species. However, it is noteworthy that there are no previous studies testing the toxicity of triazolium salts in marine eukaryotes or copepods because we consider that the LC50 values from toxicity bioassays described in the present work are fundamental for future more ecologically relevant studies.[21]

Conclusions

In summary, a 1,2,3-triazol-5-ylidene (aNHC)-based palladium(ii) complex, formed in situ from a 1,2,3-triazolium salt, is an efficient catalytic system for the formation of C–C bonds (Heck coupling) in the selective synthesis of α–β unsaturated aldehydes and ketones from the corresponding aryl halides and allylic alcohols. The efficiency of this catalytic system is reinforced by the null toxicity of the catalytic precursor (1,2,3-triazolium salt) in the marine benthic copepod Amphiascoides atopus. This result, in addition to the high thermal stability and low vapour pressure of the catalytic precursor, allows a glimpse of the low impact in air and in aquatic ecosystems resulting from one of the main waste chemicals produced in the catalytic process studied here.

Experimental section

General methods

All reagents were purchased from Aldrich Chemical Co and used without further purification unless stated otherwise. Yields refer to the chromatographically and spectroscopically (1H and 13C) homogeneous materials. The organic reactions were monitored by TLC carried out on 0.25 mm E. Merck silica gel plates. The developed TLC plates were visualised under a short-wave UV lamp or by heating after they were dipped in Ce(SO4)2. Flash column chromatography (FCC) was performed using silica gel (230–400) and employed a solvent polarity correlated with the TLC mobility. NMR experiments were conducted on a Varian 300 and Bruker 500 MHz instruments in CDCl3 (99.9% D) and CD3OD (99.8% D) as solvents; the chemical shifts (δ) were referenced to CHCl3 (7.26 ppm 1H, 77.00 ppm 13C), CD3OD (4.87 ppm 1H, 49.00 ppm 13C), or TMS (0.00 ppm). The chemical shifts are reported in parts per million (ppm).

Toxicity test

Copepods (A. atopus) were obtained from a stock maintained in laboratory culture in a 15 L flask with filtered natural seawater (35‰) under natural conditions of photoperiod (typically, 12 h light/12 h dark) and temperature (28 ± 0.5 °C), and fed with microalgae diet.[22]

To choose the test concentrations a range-finding test was performed. The definitive tests were performed in six-well culture plates. Ten copepods (ovigerous females) were randomly transferred to each well containing 5 mL of filtered seawater (35‰) at 28 ± 0.5 °C. Copepods were not fed and exposed to increasing concentrations of 1,3,4-trisubstituted-1,2,3-triazolium iodide salts 4a: 0, 30, 50, 100, 150, 200, 250, 300, 350, 450 and 550 mg L−1 for 96 h. Experiments were set up with six replicates. Mortality of copepods was checked under a stereomicroscope every 24 h. Copepods were considered dead if they did not show movement of appendages and did not show any reaction when transferred to wells with seawater without 1,3,4-trisubstituted-1,2,3-triazolium iodide salts 4a in a period of up to 20 s of observation.

Significant differences in mortality among different concentrations of 1,3,4-trisubstituted-1,2,3-triazolium iodide salts 4a were determined by one-way ANOVA with the Holm–Sidak test posteriori, performed in RStudio software. Significant differences were considered when P < 0.05. The LC50 value and its respective 95% confidence interval were determined by probit analysis method (Finney, 1971) using PASW Statistics 18 software (SPSS Inc. Chicago, IL, USA).

General procedure

Synthesis of 1,2,3-triazoles 3a and 3b[23]

To a solution of alkyne 1 (1 mmol), benzyl halide 2a or 2b (1.2 mmol), NaN3 (1.2 mmol) in a mixture of THF–H2O (20 mL, 1 : 1 v/v), were added sodium ascorbate (0.5 mmol%) and CuSO4 (0.5 mmol%). The mixture was stirred at 50 °C for 48 h. After the reaction time, the mixture was cooled to room temperature and THF was eliminated under vacuum. The resulting precipitate of the Cu complex was decomposed by addition of small portions of aqueous ammonia. Then, the mixture was diluted with ethyl acetate (25 mL), washed with NaHCO3 (7% aqueous, 2 × 10 mL) and brine (2 × 10 mL), dried over Na2SO4 and concentrated under vacuum. The crude product was purified through a silica gel column chromatography with a gradient AcOEt–hexane.

Synthesis of 1,2,3-triazolium salts 4a and 4b

A mixture of 1,2,3-triazole 3a or 3b (1.0 mmol) and CH3CN (10 mL) was treated with CH3I (20.0 mmol) at 60 °C for 48 h with vigorous stirring. Then, the white precipitate obtained was filtered and washed with acetonitrile and dried under vacuum to obtain the corresponding 1,2,3-triazolium iodide salts 4a or 4b.

General procedure for the catalytic reactions

In all Heck reactions, a DMF solution (5 mL) of 1 mmol of aryl halide 5, 1.0 mmol of allylic alcohol 6, 2.0 mmol of NaOAc, 1% mmol of Pd(OAc)2 and 1% mmol of 1,2,3-triazolium salt 4 were heated for 6 h in a 90 °C silicon oil bath equipped with a condenser system. After the reaction time, the mixture was cooled to room temperature and diluted with ethyl acetate (15 mL), washed with brine (3 × 10 mL), dried over Na2SO4 and concentrated under vacuum. The crude product was purified through a silica gel column chromatography with a gradient AcOEt–hexane.

Characterization data

2-(1-(4-Bromobenzyl)-1H-1,2,3-triazol-4-yl)pyridine 3a (80%)

1H (CDCl3, 500 MHz): δ 8.52 (ddd, J = 4.5, 1.5, 1.0 Hz, 1H), 8.16 (ddd, J = 8.0, 1.5, 1.0 Hz, 1H), 8.03 (brs, 1H), 7.75 (ddd, J = 8.0, 7.5, 1.5 Hz, 1H), 7.35 (m, 4H), 7.19 (ddd, J = 7.5, 4.5, 1.5 Hz, 1H), 5,57 (brs, 2H). NMR 13C (CDCl3, 125 MHz): δ 150.4, 149.4, 148.5, 137.0, 134.5, 129.3, 128.9, 128.4, 122.97, 122.04, 120.03, 54.5. Anal. calcd for C14H11BrN4: C, 53.35; H, 3.52; N, 17.78. Found: C, 53.30; H, 3.48; N, 17.73.

2-(1-(4-Iodobenzyl)-1H-1,2,3-triazol-4-yl)pyridine 3a (75%)

1H (CDCl3, 500 MHz): δ 8.54 (ddd, J = 4.5, 1.5, 1.0 Hz, 1H), 8.17 (ddd, J = 8.0, 1.5, 1.0 Hz, 1H), 8.04 (brs, 1H), 7.76 (ddd, J = 8.0, 7.5, 1.5 Hz, 1H), 7.71 (AA′BB′, m, 2H), 7.21 (ddd, J = 7.5, 4.5, 1.5 Hz, 1H), 7.07 (AA′BB′, m, 2H), 5.52 (brs, 2H). NMR 13C (CDCl3, 125 MHz): δ 150.2, 149.5, 149.1, 138.4, 137.0, 134.2, 130.1, 123.0, 122.0, 120.3, 94.81, 53.9. Anal. calcd for C14H11IN4: C, 46.43; H, 3.06; N, 15.47. Found: C, 46.37; H, 3.01; N, 15.42.

2-(1-(4-Bromobenzyl)-3-methyl-1H-1,2,3-triazol-3-ium-4-yl)-1-methylpyridin-1-ium iodide 4a (50%)

1H (CD3OD, 500 MHz): δ 8.98 (brs, 1H), 8.97 (m, 1H), 8.59 (m, 1H), 8.49 (dd, J = 8.0, 1.5 Hz, 1H), 8.02 (ddd, J = 7.5, 6.0, 1.5 Hz, 1H), 7.57 (AA′BB′, m, 2H), 7.39 (AA′BB′, m, 2H), 5,57 (brs, 2H), 4.56 (brs, 3H), 3.31 (brs, 1H). NMR 13C (CD3OD, 125 MHz): δ 148.2, 146.4, 140.2, 135.2, 133.2, 131.3, 129.9, 129.8, 129.6, 127.6, 123.8, 54.6, 49.2, 49.1. Anal. calcd for C16H17BrI2N4: C, 32.08; H, 2.86; Br, N, 9.35. Found: C, 32.01; H, 2.82; N, 9.30.

2-(1-(4-Iodobenzyl)-3-methyl-1H-1,2,3-triazol-3-ium-4-yl)-1-methylpyridin-1-ium iodide 4b (45%)

1H (CD3OD, 500 MHz): δ 8.98 (brs, 1H), 8.97 (m, 1H), 8.59 (m, 1H), 8.49 (dd, J = 8.0, 1.5 Hz, 1H), 8.02 (ddd, J = 7.5, 6.0, 1.5 Hz, 1H), 7.57 (AA′BB′, m, 2H), 7.39 (AA′BB′, m, 2H), 5,57 (brs, 2H), 4.58 (s, 3H), 4.56 (s, 3H). NMR 13C (CD3OD3, 125 MHz): δ 148.2, 146.5, 140.3, 135.2, 133.1, 131.4, 131.1, 129.9, 129.6, 127.6, 123.8, 54.7, 49.3, 49.2. Anal. calcd for C16H17I3N4: C, 29.75; H, 2.65; Br, N, 8.67. Found: C, 29.71; H, 2.59; N, 8.62.

1-Phenyl-3-(pyridin-3-yl)propan-1-one 7a (80% yield)

NMR 1H (CDCl3, 300 MHz): δ 8.45 (brs, 1H), 8.35 (d, J = 3.7 Hz, 1H), 7.86 (d, J = 7.0 Hz, 2H), 7.52 (m, 4H), 7.14 (dd, J = 7.5, 6.1 Hz, 1H), 3.19 (t, J = 7.2 Hz, 2H), 2.98 (t, J = 7.2 Hz, 2H). NMR 13C (CDCl3, 75 MHz): δ 198.1, 149.3, 146.9, 136.5, 136.2, 136.1, 133, 128.5, 127.7, 123.2, 39.3, 26.7. Anal. calcd for C14H13NO: C, 79.59; H, 6.20; N, 6.63. Found: C, 79.55; H, 6.17; N, 6.61.

2-(1-Methyl-3-oxopropyl)benzonitrile 7b (60% yield)

NMR 1H (CDCl3, 300 MHz): δ 9.84 (t, J = 1.4 Hz, 1H), 7.62 (m, 2H), 7.18 (m, 2H), 3.68 (sex, J = 7.2 Hz, 1H), 2.75 (dd, J = 16, 7.2 Hz, 1H), 2.66 (dd, J = 16, 7.2 Hz, 1H), 1.34 (d, J = 7.2 Hz, 3H). NMR 13C (CDCl3, 75 MHz): δ 200.3, 148.9, 133.3, 133, 127, 126.4, 117.8, 112, 50.5, 32.4, 21.4. Anal. calcd for C11H11NO: C, 76.28; H, 6.40; N, 8.09. Found: C, 76.25; H, 6.34; N, 8.01.

3-(Isoquinolin-4-il)butanal 7c (90% yield)

NMR 1H (CDCl3, 300 MHz): δ 9.85 (t, J = 1.4 Hz, 1H), 9.16 (brs, 1H), 8.47 (brs, 1H), 8.09 (dd, J = 8.2, 1.1 Hz, 1H), 8.01 (dd, J = 8.0, 1.0 Hz, 1H), 7.78 (ddd, J = 8.6, 6.8, 1.4 Hz, 1H), 7.64 (ddd, J = 8.2, 6.8, 1.0 Hz, 1H), 4.15 (sex, J = 6.8 Hz, 1H), 2.99 (ddd, J = 16.1, 5.6, 1.2 Hz, 1H), 2.89 (ddd, J = 16.8, 5.7, 1.4 Hz, 1H), 1.48 (d, J = 6.8 Hz, 1H). NMR 13C (CDCl3, 75 MHz): δ 200.9, 151.7, 140.1, 134.5, 133.7, 130.6, 128.6, 128.45, 126.9, 121.9, 50.8, 26.9, 21.4. Anal. calcd for C13H13NO: C, 78.36; H, 6.58; N, 7.03. Found: C, 78.27; H, 6.47; N, 6.96.

3-(Quinolin-3-yl)butanal 7d (75% yield)

NMR 1H (CDCl3, 300 MHz): δ 9.73 (t, J = 1.4, 1H), 8.81 (d, J = 2.3, 1H), 8.08 (d, J = 7.0, 1H), 7.97 (d, J = 2.3, 1H), 7.78 (d, J = 7.0, 1H), 7.64 (d, J = 7.0, 1H), 7.56 (d, J = 7.1, 1H), 3.57 (sex, J = 7.2 Hz, 1H), 2.88 (ddd, J = 17.2, 6.8, 1.4, 1H, Hz), 2.76 (ddd, J = 17.2, 7.4, 1.4 Hz, 1H), 1.40 (d, J = 7.0 Hz, 3H). NMR 13C (CDCl3, 75 MHz, DEPT): δ 200.6, 150.5, 146.8, 137.9, 132.8, 128.9, 127.9, 127.5, 126.7, 51.2, 31.5, 21.8. Anal. calcd for C13H13NO: C, 78.36; H, 6.58; N, 7.03. Found: C, 78.30; H, 6.51; N, 6.95.

5-(4-Oxobutan-2-yl)furan-2-carbaldehyde 7e (85% yield)

NMR 1H (CDCl3, 300 MHz): δ 9.79 (t, J = 1.6 Hz, 1H), 9.54 (brs, H1), 7.17 (d, J = 3.6 Hz, 1H), 6.29 (d, J = 3.6 Hz, 1H), 3.54 (m, 1H), 2.95 (ddd, J = 17.6, 6.4, 1.6 Hz, 1H), 2.72 (ddd, J = 17.6, 7.2, 1.6 Hz, 1H), 1.37 (d, J = 7.2 Hz, 3H). NMR 13C (CDCl3, 75 MHz): δ 199.7, 177.2, 167.3, 151.9, 123.2, 107.9, 48.5, 27.8, 18.5. Anal. calcd for C9H10O: C, 65.05; H, 6.07. Found: C, 64.96; H, 5.93.

5-(3-Pentanona)furan-2-carbaldehyde 7f (70% yield)

NMR 1H (CDCl3, 300 MHz): δ 9.65 (s, 1H), 7.33 (d, J = 3.6 Hz, 1H, H-3), 6.43 (d, J = 3.6 Hz, 1H), 3.16 (t, J = 6.8 Hz, 2H), 3.02 (t, J = 6.8 Hz, 2H), 2.62 (q, J = 7.2 Hz, 2H), 1.21 (t, J = 7.4, 3H). NMR 13C (CDCl3, 75 MHz): δ 208.9, 176.7, 161.9, 151.7, 123.6, 109.1, 39.2, 35.7, 22.2, 7.5. Anal. calcd for C10H12O3: C, 66.65; H, 6.71. Found: C, 66.61; H, 6.67.

5-(3-Oxo-3-phenylpropyl)furan-2-carbaldehyde 7g (85% yield)

NMR 1H (CDCl3, 300 MHz): δ 9.41 (brs, 1H), 7.87 (d, J = 7.5 Hz, 2H), 7.41 (m, 3H), 7.11 (d, J = 3.6 Hz, 1H), 6.25 (d, J = 3.5 Hz, 1H), 3.32 (t, J = 7.2 Hz, 2H), 3.08 (t, J = 7.2 Hz, 2H). NMR 13C (CDCl3, 75 MHz): δ 197.4, 176.7, 162.1, 151.6, 135.9, 132.9, 128.4, 127.7, 123.7, 109.1, 35.7, 22.4. Anal. calcd for C14H12O3: C, 73.67; H, 5.30. Found: C, 73.62; H, 5.27.

5-(2-Butanone)furan-2-carbaldehyde 7h (85% yield)

NMR 1H (CDCl3, 200 MHz): δ 9.44 (brs, 1H), 7.16 (d, J = 3.6 Hz, 1H), 6.45 (d, J = 3.6 Hz, 1H), 2.94 (m, 2H), 2.82 (m, 2H), 2.11 (s, 3H). NMR 13C (CDCl3, 75 MHz): δ 206.1, 176.6, 161.8, 151.7, 123.5, 109.1, 40.5, 29.6, 22.1. Anal. calcd for C9H10O: C, 65.05; H, 6.07. Found: C, 64.95; H, 5.94.

5-(2-Nitrobenzene)pentan-3-one 7i (90% yield)

(CDCl3, 300 MHz): δ 7.87 (d, J = 8.0 Hz, 1H), 7.55 (dd, J = 8.1 Hz, 7.3 Hz, 1H), 7.42 (m, 2H), 3.10 (t, J = 7.4 Hz, 2H), 2.78 (t, J = 7.6 Hz, 2H), 2.39 (q, J = 7.4 Hz, 2H), 1.01 (t, J = 7.4 Hz, 3H). NMR 13C (CDCl3, 75 MHz): δ 209.7, 149.1, 136.3, 133.1, 132.3, 127.3, 124.7, 42.6, 35.8, 27.1, 7.6. Anal. calcd for C11H13NO3: C, 63.76; H, 6.32; N, 6.76. Found: C, 63.70; H, 6.26; N, 6.71.

4-(2-Nitrobenzene)butan-2-one 7j (90% yield)

NMR 1H (CDCl3, 300 MHz): δ 7.88 (d, J = 7.2 Hz, 1H), 7.51 (dd, J = 8.0 Hz, 7.4 Hz, 1H), 7.39 (m, 2H), 3.11 (t, J = 7.2 Hz, 2H), 2.85 (t, J = 7.2 Hz, 2H), 2.14 (s, 3H). NMR 13C (CDCl3, 75 MHz): δ 206.8, 148.9, 134.3, 133.0, 132.1, 127.2, 124.6, 43.8, 29.6, 26.9. Anal. calcd for C10H11NO3: C, 62.17; H, 5.74; N, 7.25. Found: C, 62.12; H, 5.70; N, 7.19.

3-(2-Nitrobenzene)-1-phenylpropan-1-one 7k (70% yield)

NMR 1H (CDCl3, 300 MHz): δ 7.95 (m, 3H), 7.46 (m, 6H), 3.37 (m, 4H). NMR 13C (CDCl3, 75 MHz): δ 198.4, 149.2, 136.5, 136.4, 133.1, 132.5, 128.5, 127.9, 127.4, 124.8, 39.3, 27.6. Anal. calcd for C15H13NO3: C, 70.58; H, 5.13; N, 5.49. Found: C, 70.51; H, 5.05; N, 5.41.

2-(3-Oxo-3-phenylpropyl)benzonitrile 7l (50% yield)

NMR 1H (CDCl3, 300 MHz): δ 7.95 (d, J = 7.8 Hz, 1H), 7.48 (m, 7H), 7.26 (t, J = 7.9 Hz, 1H), 3.32 (m, 4H). NMR 13C (CDCl3, 75 MHz): δ 197.9, 145.1, 136.3, 133.1, 132.8, 132.7, 129.9, 128.5, 127.9, 126.7, 117.9, 112.2, 38.9, 28.6. Anal. calcd for C16H13NO: C, 81.68; H, 5.57; N, 5.95. Found: C, 81.60; H, 5.51; N, 5.89.

1-Phenyl-3-[4-(phenylcarbonyl)phenyl]propan-1-one 7m (75% yield)

NMR 1H (CDCl3, 300 MHz): δ 7.98 (d, J = 7.0 Hz, 2H), 7.79 (m, 4H), 7.45 (m, 8H), 3.35 (t, J = 7.2 Hz, 2H), 3.17 (t, J = 7.2 Hz, 2H). NMR 13C (CDCl3, 75 MHz): δ 198.7, 196.3, 146.4, 137.6, 136.5, 135.4, 133.1, 132.3, 130.4, 129.8, 128.5, 128.3, 128.1, 127.9, 39.7, 29.8. Anal. calcd for C22H18O2: C, 84.05; H, 5.77. Found: C, 83.98; H, 5.71.

2-(3-Oxopentyl)-9H-fluorene-9-one 7n (80% yield)

NMR 1H (CDCl3, 300 MHz): δ 7.62 (d, J = 7.9 Hz, 1H), 7.35 (m, 6H), 2.92 (t, J = 7.4 Hz, 2H), 2.76 (t, J = 7.4 Hz, 2H), 2.43 (q, J = 7.2 Hz, 2H), 1.05 (t, J = 7.4 Hz, 3H). NMR 13C (CDCl3, 75 MHz): δ 210.5, 194.5.8, 144.6, 142.8, 138.1, 136.9, 135.3, 134.5, 133.8, 124.8, 124.5, 124.2, 123.9, 122.6, 43.8, 29.8, 20.6, 7.5. Anal. calcd for C18H16O2: C, 81.74; H, 6.10. Found: C, 81.68; H, 6.02.

1-(Naphthalene-yl)pentan-3-one 7o (60% yield)

NMR 1H (CDCl3, 300 MHz): δ 7.95 (m, 4H), 7.62 (m, 3H), 3.48 (t, J = 7.4 Hz, 2H), 2.84 (t, J = 7.6 Hz, 2H), 2.47 (q, J = 7.4 Hz, 2H), 1.17 (t, J = 7.2 Hz, 3H). NMR 13C (CDCl3, 75 MHz): δ 210.5, 137.1, 133.6, 131.7, 128.8, 127.7, 127.1, 125.8, 125.4, 125.3, 123.3, 42.8, 35.8, 26.6, 7.6. Anal. calcd for C15H16O: C, 84.87; H, 7.60. Found: C, 84.82; H, 7.56.

4-(Naphthalene-1-yl)butan-2-one 7p (55% yield)

NMR 1H (CDCl3, 300 MHz): δ 7.85 (d, J = 7.9 Hz, 1H), 7.75 (m, 3H), 7.46 (m, 3H), 3.30 (t, J = 7.4 Hz, 2H), 2.79 (t, J = 7.2 Hz, 2H), 2.07 (s, 3H). NMR 13C (CDCl3, 75 MHz): δ 207.6, 136.8, 133.6, 131.5, 128.8, 127.8, 126.8, 125.9, 125.8, 125.4, 125.3, 44.2, 29.9, 26.6. Anal. calcd for C14H14O: C, 84.81; H, 7.12. Found: C, 84.75; H, 7.05.

4-(Isoquinolin-4-yl)butan-2-one 7q (75% yield)

NMR 1H (CDCl3, 300 MHz): δ 9.10 (s, 1H), 8.40 (s, 1H), 8.12 (d, J = 7.5 Hz, 1H), 7.7 (m, 3H), 3.27 (t, J = 7.2 Hz, 2H), 2.85 (t, J = 7.2 Hz, 2H), 2.16 (s, 3H). NMR 13C (CDCl3, 75 MHz): δ 206.8, 151.3, 144.2, 133.9, 131.2, 129.8, 128.1, 127.4, 126.6, 125.4, 42.5, 29.7, 23.1. Anal. calcd for C13H13NO: C, 78.36; H, 6.58; N, 7.03. Found: C, 78.31; H, 6.52; N, 6.97.

4-(Quinolin-3-yl)butan-2-one 7r (65% yield)

NMR 1H (CDCl3, 300 MHz): δ 8.65 (s, 1H), 8.01 (s, 1H), 7.88 (m, 1H), 7.45 (m, 3H), 2.85 (t, J = 7.4 Hz, 2H), 2.65 (t, J = 7.0 Hz, 2H), 1.95 (s, 3H). NMR 13C (CDCl3, 75 MHz): δ 206.6, 150.9, 148.9, 134.1, 130.5, 129.1, 128.1, 127.5, 127.3, 126.6, 44.1, 29.7, 26.5. Anal. calcd for C13H13NO: C, 78.36; H, 6.58; N, 7.03. Found: C, 78.32; H, 6.54; N, 6.99.

(E)-5-(3-Hydroxy-3-methylbut-1-enyl)furan-2-carbaldehyde 7s (80% yield)

NMR 1H (CDCl3, 300 MHz): δ 9.45 (s, 1H), 7.17 (d, J = 3.6 Hz, 1H), 6.64 (d, J = 15.8 Hz, 1H), 6.45 (d, J = 15.8, 1H) 6.34 (d, J = 3.6 Hz, 1H), 1.34 (s, 6H). NMR 13C (CDCl3, 75 MHz): δ 176.9, 158.3, 151.2, 143.1, 124.1, 113.8, 109.9, 70.7, 29.5. Anal. calcd for C10H12O3: C, 66.65; H, 6.71. Found: C, 66.59; H, 6.68.

2-[(E)-1-Buten-3-methyl-3-ol]benzonitrile 7t (70% yield)

NMR 1H (CDCl3, 300 MHz): δ 7.45 (m, 3H), 7.16 (dd, J = 8.0, 7.8 Hz, 2H), 6.79 (d, J = 16 Hz, 1H), 6.43 (d, J = 16 Hz, 1H), 1.34 (s, 6H). NMR 13C (CDCl3, 75 MHz): δ 142.9, 140.3, 132.7, 132.6, 127.3, 125.7, 122.2, 117.8, 110.6, 70.9, 29.4. Anal. calcd for C12H13NO: C, 76.98; H, 7.00; N, 7.48. Found: C, 76.94; H, 6.95; N, 7.43.

(E)-2-Metil-4-(quinolin-3-il)-3-buten-2-ol 7u (85% yield)

NMR 1H (CDCl3, 300 MHz): δ 8.64 (brs, 1H), 8.04 (brs, 1H), 7.9 (d, J = 8.0 Hz, 1H), 7.43 (m, 2H), 7.36 (m, 1H), 6.61 (d, J = 16.2 Hz, 1H), 6.44 (d, J = 16.2, 1H), 1.33 (s, 6H). NMR 13C (CDCl3, 75 MHz): δ 149.1, 145.6, 136.9, 129.4, 128.8, 127.3, 126.6, 140.4, 122.7, 116.7, 70.3, 29.7. Anal. calcd for C14H15NO: C, 78.84; H, 7.09; N, 6.57. Found: C, 78.79; H, 7.02; N, 6.51.

(3E,E′)-4,4′-Pyridine-3,5-di(yl)bis(3-buten-2-methyl-2-ol) 7v (75% yield)

NMR 1H (CDCl3, 300 MHz): δ 8.56 (brs, 2H), 7.98 (brs, 1H), 6.51 (d, J = 15.9 Hz, 2H), 6.38 (d, J = 15.9, 2H), 1.39 (s, 12H). NMRN 13C (CDCl3, 75 MHz): δ 149.0, 141.7, 135.3, 121.4, 120.8, 70.9, 29.8. Anal. calcd for C15H21NO2: C, 72.84; H, 8.56; N, 5.66. Found: C, 72.80; H, 8.51; N, 5.62.

Conflicts of interest

There are no conflicts to declare.