Omar M Amin1, Anshu Chaudhary2, Hirdaya S Singh2,3. 1. Institute of Parasitic Diseases, 11445 E. Via Linda 2-419, Scottsdale, AZ, 85259, USA. omaramin@aol.com. 2. Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut, Uttar Pradesh, 250004, India. 3. Maa Shakumbhari University, Saharanpur, Uttar Pradesh, 247120, India.
Abstract
PURPOSE: Immature Southwellina hispida (Van Cleave, 1925) Witenberg, 1932 from the body cavity of the paratenic host Gillichthys mirabilis Cooper (Gobiidae) in California are described. METHODS: New Scanning Electron images and features of micropores, hook and spine Gallium cut sections and chemistry using Energy Dispersive X-ray analysis (EDXA), and molecular profile are provided for the first time. The 18S rDNA and mt Cox1 sequences were performed for molecular and phylogenetic study. RESULTS: Our specimens were somewhat comparable to those reported from other paratenic hosts in Asia, Europe, and North and South America but varied in relative sizes of trunk and other structures, proboscis formula, and distribution of trunk spines. About 60 publications were reviewed of which one third included line drawings used for comparative morphometrics. In our specimens, the trunk measured 2.72-3.10 mm long by 0.92-1.07 mm wide and the proboscis 700-800 × 270-312 μm had 20-21 rows of 14-15 hooks each measuring 47-55 long by 12-15 μm wide at base anteriorly, 47-48 × 20-23 μm at middle bulge, and 43-50 × 13-20 μm basally. These measurements, among others were compared with measurements of juveniles from 13 other collections world-wide and intraspecific variability was noted especially in the shape of hook roots that were occasionally misinterpreted. EDXA showed hooks with high levels of Sulfur especially at the tip and edge of all hooks and low levels of Calcium and Phosphorus. Anterior spines had higher levels of Sodium but Gallium cut spine sections had higher levels of Calcium at middle and of Sulfur at base of spines. Micropores were variably distributed on the body wall and extended to the cortical layer of spines. Gene sequences of the 18S and the mitochondrial cytochrome c oxidase subunit 1 (cox 1) region were amplified for specimens of S. hispida. Molecular phylogenetic analysis inference from 18S rDNA and mt Cox1 gene sequences show a close relationship with previously reported myxozoan sequences available on GenBank database. Phylogenetic analysis positioned our S. hispida in a well-supported clade including other members of Polymorphidae. CONCLUSION: The present study combined morphological, morphometric and molecular data to identify S. hispida.
PURPOSE: Immature Southwellina hispida (Van Cleave, 1925) Witenberg, 1932 from the body cavity of the paratenic host Gillichthys mirabilis Cooper (Gobiidae) in California are described. METHODS: New Scanning Electron images and features of micropores, hook and spine Gallium cut sections and chemistry using Energy Dispersive X-ray analysis (EDXA), and molecular profile are provided for the first time. The 18S rDNA and mt Cox1 sequences were performed for molecular and phylogenetic study. RESULTS: Our specimens were somewhat comparable to those reported from other paratenic hosts in Asia, Europe, and North and South America but varied in relative sizes of trunk and other structures, proboscis formula, and distribution of trunk spines. About 60 publications were reviewed of which one third included line drawings used for comparative morphometrics. In our specimens, the trunk measured 2.72-3.10 mm long by 0.92-1.07 mm wide and the proboscis 700-800 × 270-312 μm had 20-21 rows of 14-15 hooks each measuring 47-55 long by 12-15 μm wide at base anteriorly, 47-48 × 20-23 μm at middle bulge, and 43-50 × 13-20 μm basally. These measurements, among others were compared with measurements of juveniles from 13 other collections world-wide and intraspecific variability was noted especially in the shape of hook roots that were occasionally misinterpreted. EDXA showed hooks with high levels of Sulfur especially at the tip and edge of all hooks and low levels of Calcium and Phosphorus. Anterior spines had higher levels of Sodium but Gallium cut spine sections had higher levels of Calcium at middle and of Sulfur at base of spines. Micropores were variably distributed on the body wall and extended to the cortical layer of spines. Gene sequences of the 18S and the mitochondrial cytochrome c oxidase subunit 1 (cox 1) region were amplified for specimens of S. hispida. Molecular phylogenetic analysis inference from 18S rDNA and mt Cox1 gene sequences show a close relationship with previously reported myxozoan sequences available on GenBank database. Phylogenetic analysis positioned our S. hispida in a well-supported clade including other members of Polymorphidae. CONCLUSION: The present study combined morphological, morphometric and molecular data to identify S. hispida.
Authors: F J Alcántar-Escalera; M García-Varela; E Vázquez-Domínguez; G Pérez-Ponce de León Journal: Mol Ecol Resour Date: 2013-03-09 Impact factor: 7.090