Flexible membrane composite based on sepiolite/chitosan/(silver nanoparticles) for enrichment and surface-enhanced Raman scattering determination of sulfamethoxazole in animal-derived food.

A sepiolite/chitosan/silver nanoparticles (Sep/CTs/AgNPs) membrane substrate has been developed for the fast separation, enrichment, and surface-enhanced Raman scattering (SERS) determination of sulfamethoxazole all-in-one. The Sep/CTs/AgNPs membrane substrate possessed the ability of rapid separation and enrichment to simplify the process for pretreatment and improve the efficiency of analysis. The grown AgNPs can provide abundant hot spots and plasmonic areas to amplify the Raman signals of target molecules effectively. The membrane substrate exhibited good stability with relative standard deviations of 5.8% and 7.1% to same batch and different batches membrane substrate, respectively,  by detecting sulfamethoxazole. The SERS method based on Sep/CTs/AgNPs membrane substrate was used for the determination of sulfamethoxazole with a linear range of 0.05-2.0 mg/L, and the limit of detection was 0.020 mg/L. The established SERS method was finally applied to the quantification of sulfamethoxazole in animal-derived food samples. Sulfamethoxazole was actually found in crucian sample with 12.4 μg/kg, and the result was confirmed by a high-performance liquid chromatography method with relative error of 5.3%. The whole process of analysis can be finished within 25 min with recoveries of 89.3-102.2%. The SERS method based on Sep/CTs/AgNPs membrane substrate provided an integrated strategy for rapid and accurate SERS analysis in food safety issues.