Literature DB >> 35467281

Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells.

Qiang Wu1, Min-Xia Ke1, Huang-Tian Yang2,3,4.   

Abstract

Extracellular vesicles (EVs) secreted by human pluripotent stem cells-derived cardiovascular progenitor cells (hPSC-CVPCs) can improve repair of infarcted hearts in mouse and nonhuman primate myocardial infarction models. To fully achieve their values, it is essential to establish an efficient method for the isolation of EVs from hPSC-CVPCs. Here we describe the protocols for efficient isolation and characterization of EVs from the conditioned medium of hPSC-CVPCs.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cardiovascular progenitor cells; Extracellular vesicles; Human embryonic stem cells; Human induced pluripotent stem cells; Human pluripotent stem cells

Mesh：

Substances：

Year:  2022        PMID： 35467281     DOI： 10.1007/978-1-0716-2341-1_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Acellular therapeutic approach for heart failure: in vitro production of extracellular vesicles from human cardiovascular progenitors.

Authors:  Nadia El Harane; Anaïs Kervadec; Valérie Bellamy; Laetitia Pidial; Hany J Neametalla; Marie-Cécile Perier; Bruna Lima Correa; Léa Thiébault; Nicolas Cagnard; Angéline Duché; Camille Brunaud; Mathilde Lemitre; Jeanne Gauthier; Alexandra T Bourdillon; Marc P Renault; Yeranuhi Hovhannisyan; Solenne Paiva; Alexandre R Colas; Onnik Agbulut; Albert Hagège; Jean-Sébastien Silvestre; Philippe Menasché; Nisa K E Renault
Journal:  Eur Heart J       Date:  2018-05-21       Impact factor: 29.983

Review 2.  Roles of exosomes in cardioprotection.

Authors:  Lucio Barile; Tiziano Moccetti; Eduardo Marbán; Giuseppe Vassalli
Journal:  Eur Heart J       Date:  2017-05-07       Impact factor: 29.983

3.  Exosome-based therapy to repair the injured heart.

Authors:  Alexandra Le Bras
Journal:  Nat Rev Cardiol       Date:  2018-07       Impact factor: 32.419

4.  Human Embryonic Stem Cell-Derived Cardiovascular Progenitors Repair Infarcted Hearts Through Modulation of Macrophages via Activation of Signal Transducer and Activator of Transcription 6.

Authors:  Jinxi Wang; Meilan Liu; Qiang Wu; Qiang Li; Ling Gao; Yun Jiang; Boxiong Deng; Wei Huang; Wei Bi; Zhongyan Chen; Y Eugene Chin; Christian Paul; Yigang Wang; Huang-Tian Yang
Journal:  Antioxid Redox Signal       Date:  2019-04-16       Impact factor: 8.401

5.  A Deep Proteome Analysis Identifies the Complete Secretome as the Functional Unit of Human Cardiac Progenitor Cells.

Authors:  Sudhish Sharma; Rachana Mishra; Grace E Bigham; Brody Wehman; Mohd M Khan; Huichun Xu; Progyaparamita Saha; Young Ah Goo; Srinivasa Raju Datla; Ling Chen; Mohan E Tulapurkar; Bradley S Taylor; Peixin Yang; Sotirios Karathanasis; David R Goodlett; Sunjay Kaushal
Journal:  Circ Res       Date:  2016-12-01       Impact factor: 17.367

6.  Cardiovascular progenitor-derived extracellular vesicles recapitulate the beneficial effects of their parent cells in the treatment of chronic heart failure.

Authors:  Anaïs Kervadec; Valérie Bellamy; Nadia El Harane; Lousineh Arakélian; Valérie Vanneaux; Isabelle Cacciapuoti; Hany Nemetalla; Marie-Cécile Périer; Hadi D Toeg; Adèle Richart; Mathilde Lemitre; Min Yin; Xavier Loyer; Jérôme Larghero; Albert Hagège; Marc Ruel; Chantal M Boulanger; Jean-Sébastien Silvestre; Philippe Menasché; Nisa K E Renault
Journal:  J Heart Lung Transplant       Date:  2016-01-19       Impact factor: 10.247

Review 7.  Biological Nanotherapeutics for Liver Disease.

Authors:  Julia Driscoll; Cody Wehrkamp; Yu Ota; Jonathan N Thomas; Irene K Yan; Tushar Patel
Journal:  Hepatology       Date:  2021-08-30       Impact factor: 17.425

Review 8.  Native and bioengineered extracellular vesicles for cardiovascular therapeutics.

Authors:  Ricardo Cerqueira de Abreu; Hugo Fernandes; Paula A da Costa Martins; Susmita Sahoo; Costanza Emanueli; Lino Ferreira
Journal:  Nat Rev Cardiol       Date:  2020-06-01       Impact factor: 32.419

9.  Extracellular vesicles from human embryonic stem cell-derived cardiovascular progenitor cells promote cardiac infarct healing through reducing cardiomyocyte death and promoting angiogenesis.

Authors:  Qiang Wu; Jinxi Wang; Wilson Lek Wen Tan; Yun Jiang; Shihui Wang; Qiang Li; Xiujian Yu; Jiliang Tan; Shenyan Liu; Peng Zhang; Zenia Tiang; Zhongyan Chen; Roger Sik-Yin Foo; Huang-Tian Yang
Journal:  Cell Death Dis       Date:  2020-05-11       Impact factor: 8.469

Review 10.  Prospects and challenges of extracellular vesicle-based drug delivery system: considering cell source.

Authors:  Wanrong Meng; Chanshi He; Yaying Hao; Linlin Wang; Ling Li; Guiquan Zhu
Journal:  Drug Deliv       Date:  2020-12       Impact factor: 6.419
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