| Literature DB >> 35467280 |
Andrew Lai1, Carlos Palma1, Alexis Salas2, Flavio Carrion3, Carlos Salomon4,5,6.
Abstract
Extracellular vesicles (EVs) carry a wide range of molecules, such as proteins, RNAs, and DNA. EVs are secreted from a wide range of cells, including placental cells. Interestingly, EVs secreted from placental cells have been identified in maternal circulation as early as 6 weeks of gestation, and their concentration increases with the gestational age. While there is growing interest in elucidating the role of exosomes during normal and complicated pregnancies, progress in the field has been delayed because of the inability to quantify placental EVs from the maternal circulation. Recent reports have demonstrated the presence of placental-type alkaline phosphatase (PLAP) EVs only in the blood of pregnant women, indicating that PLAP is a marker to identify EVs secreted from the placenta. Therefore, here we describe a workflow to quantify placental EVs from maternal circulation using a targeted proteomics approach based on selecting specific peptides identified in the PLAP protein.Entities:
Keywords: Multiple reaction monitoring high resolution (MRMHR); Placental alkaline phosphatase (PLAP); Placental extracellular vesicles; Targeted mass spectrometry
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Year: 2022 PMID： 35467280 DOI： 10.1007/978-1-0716-2341-1_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745