Literature DB >> 35467277

Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography.

Kaloyan Takov1, I-Jou Teng2, Manuel Mayr3.   

Abstract

Circulating extracellular vesicles (EVs) are gaining increased attention as carriers of proteins, nucleic acids, and lipids. Blood contains EVs from different cell sources that constitute an attractive target for biomarker studies. However, there is no consensus on the best approach to isolate EVs from blood. Non-EV proteins and lipoproteins in plasma/serum tend to contaminate isolated EVs and confound functional experiments. Here we describe a single-step, high-performance size-exclusion chromatography procedure for separation of EVs from most lipoproteins and non-EV proteins, and compare it to ultracentrifugation, still the most commonly used method for EV isolation.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Exosomes; Extracellular vesicles; HPLC; Lipoproteins; Plasma; Proteomics; Size-exclusion chromatography

Mesh：

Substances：

Year:  2022        PMID： 35467277     DOI： 10.1007/978-1-0716-2341-1_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

Review 1.  Circulating Extracellular Vesicles in Human Disease.

Authors:  Ravi Shah; Tushar Patel; Jane E Freedman
Journal:  N Engl J Med       Date:  2018-09-06       Impact factor: 91.245

Review 2.  What Are We Looking At? Extracellular Vesicles, Lipoproteins, or Both?

Authors:  Jens B Simonsen
Journal:  Circ Res       Date:  2017-09-29       Impact factor: 17.367

Review 3.  What is the blood concentration of extracellular vesicles? Implications for the use of extracellular vesicles as blood-borne biomarkers of cancer.

Authors:  Kasper Bendix Johnsen; Johann Mar Gudbergsson; Thomas Lars Andresen; Jens Bæk Simonsen
Journal:  Biochim Biophys Acta Rev Cancer       Date:  2018-12-07       Impact factor: 10.680

Review 4.  Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication.

Authors:  Mathilde Mathieu; Lorena Martin-Jaular; Grégory Lavieu; Clotilde Théry
Journal:  Nat Cell Biol       Date:  2019-01-02       Impact factor: 28.824

5.  Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen.

Authors:  Yusuke Yoshioka; Nobuyoshi Kosaka; Yuki Konishi; Hideki Ohta; Hiroyuki Okamoto; Hikaru Sonoda; Ryoji Nonaka; Hirofumi Yamamoto; Hideshi Ishii; Masaki Mori; Koh Furuta; Takeshi Nakajima; Hiroshi Hayashi; Hajime Sugisaki; Hiroko Higashimoto; Takashi Kato; Fumitaka Takeshita; Takahiro Ochiya
Journal:  Nat Commun       Date:  2014-04-07       Impact factor: 14.919

6.  Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods.

Authors:  Tamás Baranyai; Kata Herczeg; Zsófia Onódi; István Voszka; Károly Módos; Nikolett Marton; György Nagy; Imre Mäger; Matthew J Wood; Samir El Andaloussi; Zoltán Pálinkás; Vikas Kumar; Péter Nagy; Ágnes Kittel; Edit Irén Buzás; Péter Ferdinandy; Zoltán Giricz
Journal:  PLoS One       Date:  2015-12-21       Impact factor: 3.240

7.  Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection.

Authors:  Barbara W Sódar; Ágnes Kittel; Krisztina Pálóczi; Krisztina V Vukman; Xabier Osteikoetxea; Katalin Szabó-Taylor; Andrea Németh; Beáta Sperlágh; Tamás Baranyai; Zoltán Giricz; Zoltán Wiener; Lilla Turiák; László Drahos; Éva Pállinger; Károly Vékey; Péter Ferdinandy; András Falus; Edit Irén Buzás
Journal:  Sci Rep       Date:  2016-04-18       Impact factor: 4.379

8.  Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey.

Authors:  Chris Gardiner; Dolores Di Vizio; Susmita Sahoo; Clotilde Théry; Kenneth W Witwer; Marca Wauben; Andrew F Hill
Journal:  J Extracell Vesicles       Date:  2016-10-31

9.  Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential.

Authors:  Kaloyan Takov; Derek M Yellon; Sean M Davidson
Journal:  J Extracell Vesicles       Date:  2018-12-28

10.  Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium.

Authors:  Liang Dong; Richard C Zieren; Kengo Horie; Chi-Ju Kim; Emily Mallick; Yuezhou Jing; Mingxiao Feng; Morgan D Kuczler; Jordan Green; Sarah R Amend; Kenneth W Witwer; Theo M de Reijke; Yoon-Kyoung Cho; Kenneth J Pienta; Wei Xue
Journal:  J Extracell Vesicles       Date:  2021-01-15
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