Literature DB >> 3546482

Immunolocalization of glyceraldehyde-3-phosphate dehydrogenase, hexokinase, and carboxypeptidase Y in yeast cells at the ultrastructural level.

E van Tuinen, H Riezman.   

Abstract

We have developed a simple and effective method to embed whole yeast cells in Lowicryl resins with excellent ultrastructural and antigenic preservation. Using affinity-purified antibodies eluted from electrophoretically separated proteins transferred to nitrocellulose, we have shown by immunoelectron microscopy that two glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase and hexokinase, are present in the cytoplasm and the nucleus. Carboxypeptidase Y is localized in the yeast vacuole. These results agree with earlier localization studies based on subcellular fractionation.

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Year:  1987        PMID： 3546482     DOI： 10.1177/35.3.3546482

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  32 in total

1.  Specific retrieval of the exocytic SNARE Snc1p from early yeast endosomes.

Authors:  M J Lewis; B J Nichols; C Prescianotto-Baschong; H Riezman; H R Pelham
Journal:  Mol Biol Cell       Date:  2000-01       Impact factor: 4.138

2.  Fate of highly expressed proteins destined to peroxisomes in Saccharomyces cerevisiae.

Authors:  A Hartig; M Ogris; G Cohen; M Binder
Journal:  Curr Genet       Date:  1990-07       Impact factor: 3.886

3.  Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.

Authors:  Helga Grötsch; Jonathan P Giblin; Fatima-Zahra Idrissi; Isabel-María Fernández-Golbano; John R Collette; Thomas M Newpher; Virginia Robles; Sandra K Lemmon; María-Isabel Geli
Journal:  EMBO J       Date:  2010-07-20       Impact factor: 11.598

4.  Topology of penicillin-binding protein 1b of Escherichia coli and topography of four antigenic determinants studied by immunocolabeling electron microscopy.

Authors:  T den Blaauwen; N Nanninga
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

5.  Ultrastructural localization of sialylated glycoconjugates in cells of the salamander olfactory mucosa using lectin cytochemistry.

Authors:  J D Foster; M L Getchell; T V Getchell
Journal:  Cell Tissue Res       Date:  1992-01       Impact factor: 5.249

Review 6.  Immunogold labeling of yeast cells: an efficient tool for the study of protein targeting and morphological alterations due to overexpression and inactivation of genes.

Authors:  M Binder; A Hartig; T Sata
Journal:  Histochem Cell Biol       Date:  1996-07       Impact factor: 4.304

7.  Morphology of the yeast endocytic pathway.

Authors:  C Prescianotto-Baschong; H Riezman
Journal:  Mol Biol Cell       Date:  1998-01       Impact factor: 4.138

8.  Localization of Membrane Proteins in the Cyanobacterium Synechococcus sp. PCC7942 (Radial Asymmetry in the Photosynthetic Complexes).

Authors:  D. M. Sherman; T. A. Troyan; L. A. Sherman
Journal:  Plant Physiol       Date:  1994-09       Impact factor: 8.340

9.  Characterization of the Saccharomyces Golgi complex through the cell cycle by immunoelectron microscopy.

Authors:  D Preuss; J Mulholland; A Franzusoff; N Segev; D Botstein
Journal:  Mol Biol Cell       Date:  1992-07       Impact factor: 4.138

10.  Salmonella enterica serovar Typhimurium adhesion and cytotoxicity during epithelial cell stress is reduced by Lactobacillus rhamnosus GG.

Authors:  Kristin M Burkholder; Arun K Bhunia
Journal:  Gut Pathog       Date:  2009-07-09       Impact factor: 4.181
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