Literature DB >> 35451750

Measurement of VEGF Content in Exosomes and Subsequent Tumor Tubulogenesis and In Vivo Angiogenesis Functional Assays.

Ye Zeng1, Xiaoqiang Du2, Wenli Jiang2, Yan Qiu2.   

Abstract

Vascular endothelial growth factor (VEGF) plays a vital role in angiogenesis, and is also involved in tumor cell growth and immunosuppression, showing very complex roles. VEGF-exosomes are released by tumor endothelial cells (ECs) following anti-angiogenesis therapies (AATs). Transwell assays enable the detection of migration and invasion capacities of tumor cells. Matrigel assays are used to evaluate the angiogenesis capacities of ECs. Here we describe the detection of VEGF content in exosomes by nano-flow cytometry, enzyme-linked immunosorbent assay (ELISA), and western blotting, and demonstrate the procedure for detection of the colon formation of tumor cells induced by exosomes, the angiogenesis of tumor cells co-cultured with ECs, the angiogenesis of tumor cells induced by exosomes in Matrigel assay in vitro and tumor xenografts.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Angiogenesis; Endothelial cells; Exosomes; Tumor; VEGF

Mesh：

Substances：

Year:  2022        PMID： 35451750     DOI： 10.1007/978-1-0716-2217-9_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  18 in total

1.  Tumour-secreted miR-9 promotes endothelial cell migration and angiogenesis by activating the JAK-STAT pathway.

Authors:  Guanglei Zhuang; Xiumin Wu; Zhaoshi Jiang; Ian Kasman; Jenny Yao; Yinghui Guan; Jason Oeh; Zora Modrusan; Carlos Bais; Deepak Sampath; Napoleone Ferrara
Journal:  EMBO J       Date:  2012-07-06       Impact factor: 11.598

2.  Axitinib plus pembrolizumab in patients with advanced sarcomas including alveolar soft-part sarcoma: a single-centre, single-arm, phase 2 trial.

Authors:  Breelyn A Wilky; Matteo M Trucco; Ty K Subhawong; Vaia Florou; Wungki Park; Deukwoo Kwon; Eric D Wieder; Despina Kolonias; Andrew E Rosenberg; Darcy A Kerr; Efrosyni Sfakianaki; Mark Foley; Jaime R Merchan; Krishna V Komanduri; Jonathan C Trent
Journal:  Lancet Oncol       Date:  2019-05-08       Impact factor: 41.316

Review 3.  Vascular endothelial growth factor and angiogenesis.

Authors:  Ann Hoeben; Bart Landuyt; Martin S Highley; Hans Wildiers; Allan T Van Oosterom; Ernst A De Bruijn
Journal:  Pharmacol Rev       Date:  2004-12       Impact factor: 25.468

4.  Ten years of anti-vascular endothelial growth factor therapy.

Authors:  Napoleone Ferrara; Anthony P Adamis
Journal:  Nat Rev Drug Discov       Date:  2016-01-18       Impact factor: 84.694

5.  Glioblastoma stem-like cells secrete the pro-angiogenic VEGF-A factor in extracellular vesicles.

Authors:  Lucas Treps; Raul Perret; Sébastien Edmond; Damien Ricard; Julie Gavard
Journal:  J Extracell Vesicles       Date:  2017-08-08

6.  Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer.

Authors:  Sushrut Kamerkar; Valerie S LeBleu; Hikaru Sugimoto; Sujuan Yang; Carolina F Ruivo; Sonia A Melo; J Jack Lee; Raghu Kalluri
Journal:  Nature       Date:  2017-06-07       Impact factor: 49.962

Review 7.  Extracellular Vesicles in Angiogenesis.

Authors:  Dilyana Todorova; Stéphanie Simoncini; Romaric Lacroix; Florence Sabatier; Françoise Dignat-George
Journal:  Circ Res       Date:  2017-05-12       Impact factor: 17.367

8.  Axitinib in Combination With Toripalimab, a Humanized Immunoglobulin G4 Monoclonal Antibody Against Programmed Cell Death-1, in Patients With Metastatic Mucosal Melanoma: An Open-Label Phase IB Trial.

Authors:  Xinan Sheng; Xieqiao Yan; Zhihong Chi; Lu Si; Chuanliang Cui; Bixia Tang; Siming Li; Lili Mao; Bin Lian; Xuan Wang; Xue Bai; Li Zhou; Yan Kong; Jie Dai; Kai Wang; Xiongwen Tang; Huaning Zhou; Hai Wu; Hui Feng; Sheng Yao; Keith T Flaherty; Jun Guo
Journal:  J Clin Oncol       Date:  2019-08-12       Impact factor: 44.544

9.  Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers.

Authors:  Johan Skog; Tom Würdinger; Sjoerd van Rijn; Dimphna H Meijer; Laura Gainche; Miguel Sena-Esteves; William T Curry; Bob S Carter; Anna M Krichevsky; Xandra O Breakefield
Journal:  Nat Cell Biol       Date:  2008-11-16       Impact factor: 28.824

10.  Tumour exosome integrins determine organotropic metastasis.

Authors:  Ayuko Hoshino; Bruno Costa-Silva; Tang-Long Shen; Goncalo Rodrigues; Ayako Hashimoto; Milica Tesic Mark; Henrik Molina; Shinji Kohsaka; Angela Di Giannatale; Sophia Ceder; Swarnima Singh; Caitlin Williams; Nadine Soplop; Kunihiro Uryu; Lindsay Pharmer; Tari King; Linda Bojmar; Alexander E Davies; Yonathan Ararso; Tuo Zhang; Haiying Zhang; Jonathan Hernandez; Joshua M Weiss; Vanessa D Dumont-Cole; Kimberly Kramer; Leonard H Wexler; Aru Narendran; Gary K Schwartz; John H Healey; Per Sandstrom; Knut Jørgen Labori; Elin H Kure; Paul M Grandgenett; Michael A Hollingsworth; Maria de Sousa; Sukhwinder Kaur; Maneesh Jain; Kavita Mallya; Surinder K Batra; William R Jarnagin; Mary S Brady; Oystein Fodstad; Volkmar Muller; Klaus Pantel; Andy J Minn; Mina J Bissell; Benjamin A Garcia; Yibin Kang; Vinagolu K Rajasekhar; Cyrus M Ghajar; Irina Matei; Hector Peinado; Jacqueline Bromberg; David Lyden
Journal:  Nature       Date:  2015-10-28       Impact factor: 49.962
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