Literature DB >> 35451748

Multiparameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells.

Theresa Connor1, Nerida Sleebs1, Zerina Lokmic-Tomkins2,3.   

Abstract

Multiparameter fluorescence-activated cell sorting (FACS) procedure separates target cells from a total population of cells by using specific signatures that the target cell expresses on their cell surface. For human lymphatic endothelial cells (LECs) this relates to cell surface expression of the CD34LowCD31HighVEGFR-3HighPodoplaninHigh profile that permits their separation from blood vascular endothelial cells and other cells likely to be present in the digested tissue sample. In addition, FACS allows the evaluation of LEC size, volume, granularity, and purity at the time of sorting.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cell culture; Cell surface markers; FACS; Lymphatic endothelial cell

Mesh：

Substances：

Year:  2022        PMID： 35451748     DOI： 10.1007/978-1-0716-2217-9_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  A protocol for isolation and culture of human umbilical vein endothelial cells.

Authors:  Bruno Baudin; Arnaud Bruneel; Nelly Bosselut; Michel Vaubourdolle
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

2.  Parental Experience of Prenatal Diagnosis of Lymphatic Malformation.

Authors:  Z Lokmic; L Hallenstein; A J Penington
Journal:  Lymphology       Date:  2017       Impact factor: 1.286

3.  Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells.

Authors:  Zerina Lokmic
Journal:  Methods Mol Biol       Date:  2016

Review 4.  Utilizing lymphatic cell markers to visualize human lymphatic abnormalities.

Authors:  Zerina Lokmic
Journal:  J Biophotonics       Date:  2017-12-18       Impact factor: 3.207

5.  Isolation of human lymphatic malformation endothelial cells, their in vitro characterization and in vivo survival in a mouse xenograft model.

Authors:  Zerina Lokmic; Geraldine M Mitchell; Nicholas Koh Wee Chong; Jacqueline Bastiaanse; Yi-Wen Gerrand; Yiping Zeng; Elizabeth D Williams; Anthony J Penington
Journal:  Angiogenesis       Date:  2013-07-25       Impact factor: 9.596
  5 in total

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