Nathalie Atallah1,2,3, Claire Gaudichon1, Audrey Boulier2, Alain Baniel2, Dalila Azzout-Marniche1, Nadezda Khodorova1, Catherine Chaumontet1, Julien Piedcoq1, Martin Chapelais1, Juliane Calvez4. 1. Université Paris-Saclay, AgroParisTech, INRAE, UMR PNCA, 75005, Paris, France. 2. Ingredia S.A., 62033, Arras Cedex, France. 3. UMR Transfrontalière BioEcoAgro N°1158, University Lille, INRAE, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d'Opale, ICV - Institut Charles Viollette, 59000, Lille, France. 4. Université Paris-Saclay, AgroParisTech, INRAE, UMR PNCA, 75005, Paris, France. juliane.calvez@agroparistech.fr.
Abstract
PURPOSE: Physiological parameters such as adiposity and age are likely to influence protein digestion and utilization. The aim of this study was to evaluate the combined effects of age and adiposity on casein protein and amino acid true digestibility and its postprandial utilization in rats. METHODS: Four groups were included (n = 7/8): 2 months/normal adiposity, 2 months/high adiposity, 11 months/normal adiposity and 11 months/high adiposity. Rats were given a calibrated meal containing 15N-labeled casein (Ingredia, Arras, France) and were euthanized 6 h later. Digestive contents were collected to assess protein and amino acid digestibilities. 15N enrichments were measured in plasma and urine to determine total body deamination. Fractional protein synthesis rate (FSR) was determined in different organs using a flooding dose of 13C valine. RESULTS: Nitrogen and amino acid true digestibility of casein was around 95-96% depending on the group and was increased by 1% in high adiposity rats (P = 0.04). Higher adiposity levels counteracted the increase in total body deamination (P = 0.03) that was associated with older age. Significant effects of age (P = 0.006) and adiposity (P = 0.002) were observed in the muscle FSR, with age decreasing it and adiposity increasing it. CONCLUSION: This study revealed that a higher level of adiposity resulted in a slight increase in protein and individual amino acid true digestibility values and seemed to compensate for the metabolic postprandial protein alterations observed at older age.
PURPOSE: Physiological parameters such as adiposity and age are likely to influence protein digestion and utilization. The aim of this study was to evaluate the combined effects of age and adiposity on casein protein and amino acid true digestibility and its postprandial utilization in rats. METHODS: Four groups were included (n = 7/8): 2 months/normal adiposity, 2 months/high adiposity, 11 months/normal adiposity and 11 months/high adiposity. Rats were given a calibrated meal containing 15N-labeled casein (Ingredia, Arras, France) and were euthanized 6 h later. Digestive contents were collected to assess protein and amino acid digestibilities. 15N enrichments were measured in plasma and urine to determine total body deamination. Fractional protein synthesis rate (FSR) was determined in different organs using a flooding dose of 13C valine. RESULTS: Nitrogen and amino acid true digestibility of casein was around 95-96% depending on the group and was increased by 1% in high adiposity rats (P = 0.04). Higher adiposity levels counteracted the increase in total body deamination (P = 0.03) that was associated with older age. Significant effects of age (P = 0.006) and adiposity (P = 0.002) were observed in the muscle FSR, with age decreasing it and adiposity increasing it. CONCLUSION: This study revealed that a higher level of adiposity resulted in a slight increase in protein and individual amino acid true digestibility values and seemed to compensate for the metabolic postprandial protein alterations observed at older age.
Authors: Melissa M Markofski; Jared M Dickinson; Micah J Drummond; Christopher S Fry; Satoshi Fujita; David M Gundermann; Erin L Glynn; Kristofer Jennings; Douglas Paddon-Jones; Paul T Reidy; Melinda Sheffield-Moore; Kyle L Timmerman; Blake B Rasmussen; Elena Volpi Journal: Exp Gerontol Date: 2015-02-28 Impact factor: 4.032
Authors: Stefan H M Gorissen; Jorn Trommelen; Imre W K Kouw; Andrew M Holwerda; Bart Pennings; Bart B L Groen; Benjamin T Wall; Tyler A Churchward-Venne; Astrid M H Horstman; René Koopman; Nicholas A Burd; Cas J Fuchs; Marlou L Dirks; Peter T Res; Joan M G Senden; Jan M J M Steijns; Lisette C P G M de Groot; Lex B Verdijk; Luc J C van Loon Journal: J Nutr Date: 2020-02-18 Impact factor: 4.798
Authors: Benjamin Toby Wall; Stefan H Gorissen; Bart Pennings; René Koopman; Bart B L Groen; Lex B Verdijk; Luc J C van Loon Journal: PLoS One Date: 2015-11-04 Impact factor: 3.240