Wheat flour, an inexpensive medium for in vitro cultivation of coprophilous fungus Coprinopsis cinerea.

Coprinopsis cinerea, a coprophilous basidiomycetous fungus generally called as inky cap mushroom is used as a model organism to study the evolution of fruiting bodies in higher fungi. Herbivorous animal dung is a major source of Coprinopsis cinerea, as it contains high carbon, nitrogen, and phosphorus elements. Due to the extensive application of Coprinopsis cinerea in Genetics, Molecular Biology and Microbial Biotechnology it is necessary to explore a suitable inexpensive medium for its in vitro cultivation. In our present study, we found that 2% wheat flour medium supported the vegetative growth and induced the fruiting body formation within 10 days at 30°C, pH 6 under dark compared to malt extract amended media. The number of fruiting bodies and biomass of fruiting bodies were also found higher in wheat flour medium compared to other media tested with similar cultural conditions.