| Literature DB >> 35425915 |
Abstract
Globally, the shrimp farming industry faces increasing challenges and pressure to reduce the broken shrimps and maintain a healthier pond environment. Shrimps lack an adaptive immune system to combat invading pathogens due to an imbalance in beneficial gut microbiota. The use of top-dressing agents like probiotics and pond optimizes is an alternative strategy to improve the innate immune system leading produce disease-free shrimp in international markets. The cost of top-dressing agents is accounted for 20% of the production cost and therefore, the development of top-dressing automation technology is important to maintain and improve the financial and environmental viability of shrimp sustainable farming. This perspective described several sensor-based aquaculture technologies for on-farm management systems but sustainability in the aquaculture industry is not yet achieved in practice. The present technology is a new invention to reduce labor and production costs required for reducing bacterial and organic loads in Biofloc shrimp cultures. Aquaculture automation system disperses the top-dressing agents to the shrimp ponds based on the signals received from microbial and environmental sensors. Continuous monitoring of shrimp growth, mortality, immune responses, diseases, and pond water quality parameters will fetch larger profits with additional savings on labor and production costs for sustainable shrimp aquaculture in India.Entities:
Keywords: Automation; Disease control; Immunity; Probiotics; Shrimp; Top-dressing
Year: 2021 PMID: 35425915 PMCID: PMC8142868 DOI: 10.1007/s43621-021-00036-9
Source DB: PubMed Journal: Discov Sustain ISSN: 2662-9984
Details of PCR and RT-PCR primers for studying probiotics competency, biofilm-forming efficiency, Biofloc microbiota, and shrimp diseases
| Target specifications | Primer | Primer sequence |
|---|---|---|
| Microbiota | ||
| Bacteria | 1114-F | CGGCAACGAGCGCAACCC |
| 1275-R | CCATTGTAGCACGTGTGTAGCC | |
| Methanogens | McrA-F | TTCGGTGGATCDCARAGRGC |
| McrA-R | GBARGTCGWAWCCGTAGAATCC | |
| Probe | ARGCACCKAACAMCATGGACACWGT | |
| Lactobacillus | lacto-F | GAGGCAGCAGTAGGGAATCTTC |
| lacto-R | GGCCAGTTACTACCTCTATCCTTCTTC | |
| Immune-response and probiotic competency related genes | ||
| F1F0-ATPase β-subunit | MGB-lacto | ATGGAGCAACGCCGC |
| atpD-F | GCCAACCTGGTTCGTATGTG | |
| atpD-R | ACCACGTCGTCGATCTTACC | |
| Bile salt hydrolase | bsh-F | ATGGGCGGACTAGGATTACC |
| bsh-R | TGCCACTCTCTGTCTGCATC | |
| Mucin binding protein | mub-F | GTAGTTACTCAGTGACGATCAATG |
| mub-R | TAATTGTAAAGGTATAATCGGAGG | |
| Mucus adhesion-promoting protein | mapA-F | TGGATTCTGCTTGAGGTAAG |
| mapA-R | GACTAGTAATAACGCGACCG | |
| Glyceraldehyde-3-phosphate dehydrogenase | Gadph-bac-F | ACTGAATTAGTTGCTATCTTAGAC |
| Nisin biosynthesis protein | nisB-F | GGGAGAGTTGCCGATGTTGT |
| nisB-R | TAAAGCCACTCGTTAAAGGGCAAT | |
| 18 S rRNA (Shrimp) | 18 S ShrRNA-F | GAGACGGCTACCACATCTAAG |
| 18 S ShrRNA-R | ATACGCTAGTGGAGCTGGA | |
| β-Actin (Shrimp) | LvActin-F | CCACGAGACCACCTACAAC |
| LvActin-B | AGCGAGGGCAGTGATTTC | |
| Shrimp disease-coding genes | ||
| White spot syndrome virus | WSSV-F | ATCATGGCTGCTTCACAGAC |
| WSSV-R | GGCTGGAGAGGACAAGACAT | |
| Infectious hypodermal and hematopoietic necrosis virus | IHHNV-F | TCCAACACTTAGTCAAAACCAA |
| IHHNV-R | TGTCTGCTACGATGATTATCCA | |
| Monodon baculovirus | MBV-F | CGATTCCATATCGGCCGAATA |
| MBV-R | TTGGCATGCACTCCCTGAGAT | |
| Hepatopancreatic parvovirus | HPV-F | GCATTACAAGAGCCAAGCAG |
| HPV-R | ACACTCAGCCTCTACCTTGT | |
| Infectious myonecrosis virus | IMNV-F | CGACGCTGCTAACCATACAA |
| IMNV-R | ACTCGGCTGTTCGATCAAGT | |
| Yellowhead virus | YHV-F | CCGCTAATTTCAAAAACTACG |
| YHV-R | AAGGTGTTATGTCGAGGAAGT | |
| Taura syndrome virus | TSV-F | AAGTAGACAGCCGCGCTT |
| TSV-R | TCAATGAGAGCTTGGTCC | |
| Necrotizing hepatopancreatitis bacterium | NHPB-F | CGTTGGAGGTTCGTCCTTCAGT |
| NHPB-R | GCCATGAGGACCTGACATCATC | |
| Acute hepatopancreatic necrosis disease | AHPND-F | ATGAGTAACAATATAAAACATGAAAC |
| AHPND-R | ACGATTTCGACGTTCCCCAA | |
| Enterocytozoon hepatopenaei | EHP-F | CAGCAGGCGCGAAAATTGTCCA |
| EHP-R | AAGAGATATTGTATTGCGCTTGCTG | |
Commercial aquaculture sensors and their specifications
| Parameters | YSI Inc. | Xylem Inc. | IN SITU Inc. | ATLAS Scientific | ||||
|---|---|---|---|---|---|---|---|---|
| Model | Range | Model | Range | Model | Range | Model | Range | |
| Temperature | 006560 | 1–50 °C | 4060R | 1–40 °C | 0063490 | 1–50 °C | V 1.2 | 1–99 °C |
| pH | 577602 | 0–14 | 6589FR | 0–14 | 0060000 | 0–14 | V4.2 | 0–14 |
| ORP | 006565 | ± 300 mV | SensoLyt®SEA | ± 2000 mV | 0063470 | ± 1400 mV | V 2.2 | ± 2000 mV |
| Conductivity | 599827 | 0–100 mS/cm | 4119ASW | 0–75 mS/cm | 0063460 | 0–350 mS/cm | V 3.7 | 0.07–50 mS/cm |
| DO | 006562 | 0–50 mg/L | FDO 700 IQ | 0–20 mg/L | RDO®Blue | 0–60 mg/L | V 4.6 | 0–100 mg/L |
| Turbidity | 626901 | 0–4000 FNU | WQ 730 | 0-1000 FTU | 0063480 | 0–4000 FNU | – | – |
| Ammonia | 626906 | 0–200 mg/L | Tru Lab 1320 | 0.02–17,000 mg/L | 0033700 | 0–10,000 mg/L | – | – |
| Nitrate | 608090 | 0–10 mg/L | YSI MultiLab 4010-2 | 0.4–62000 mg/L | 0033710 | 0–40,000 mg/L as N | – | – |
| Chlorophyll | 599102-01 | 0–100 µg/L | YSI 6025 | 0–400 µg/L | 0038900 | 0–1000 µg/L | – | – |
| BGA | 626210 | 0–100 RFU | YSI 6031 | 0–100 RFU | 0038930 | 0–100 RFU | – | – |
Fig. 1Schematic representation of aquaculture automation designing for Biofloc production of shrimp
Fig. 2depicts the overview of experimental aquaculture automation system for Biofloc production of shrimp (Place: Bharathidasan University, Tiruchirappalli, India)
Fig. 3Top-dressing system with stirring and dispersion units to be used in an aquaculture automation system for Biofloc production of shrimp
Fig. 4The proposed model for a top-dressing automation system for Biofloc production of shrimp