Literature DB >> 3542297

Microbiological assay for vitamin B12 with use of a colistin-sulfate-resistant organism.

B P Kelleher, K G Walshe, J M Scott, S D O'Broin.   

Abstract

In this simplified microbiological assay for serum vitamin B12, Lactobacillus leichmanii (NCIB 8117) adapted to tolerate high concentrations (500 mg/L) of the polymyxin antibiotic colistin sulfate is used. Results were similar in parallel experiments in which we used both the parent strain of L. leichmanii (NCIB 8117), and the new adapted strain. Evaluation of assay performance showed excellent analytical recovery of added cyanocobalamin (97%, SD 3%) and good interassay and intra-assay precision (CV less than 5%). This modified assay system obviates the need to sterilize culture medium and glassware. Consequently, assay manipulations may be carried out openly, without aseptic precautions. Moreover, this adapted organism would be suitable for use in an automated microbiological assay system.

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Year:  1987        PMID: 3542297

Source DB:  PubMed          Journal:  Clin Chem        ISSN: 0009-9147            Impact factor:   8.327


  26 in total

1.  The FUT2 secretor variant p.Trp154Ter influences serum vitamin B12 concentration via holo-haptocorrin, but not holo-transcobalamin, and is associated with haptocorrin glycosylation.

Authors:  Aneliya Velkova; Jennifer E L Diaz; Faith Pangilinan; Anne M Molloy; James L Mills; Barry Shane; Erica Sanchez; Conal Cunningham; Helene McNulty; Cheryl D Cropp; Joan E Bailey-Wilson; Alexander F Wilson; Lawrence C Brody
Journal:  Hum Mol Genet       Date:  2017-12-15       Impact factor: 6.150

2.  Streptozotocin-induced diabetes is not a model for methylmalonic acidaemia.

Authors:  L B Bailey; A Molloy; J Scott; D Rice
Journal:  J Inherit Metab Dis       Date:  1989       Impact factor: 4.982

3.  A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults.

Authors:  Clovis Paniz; Juliano Felix Bertinato; Maylla Rodrigues Lucena; Eduardo De Carli; Patrícia Mendonça da Silva Amorim; Guilherme Wataru Gomes; Cecília Zanin Palchetti; Maria Stella Figueiredo; Christine M Pfeiffer; Zia Fazili; Ralph Green; Elvira Maria Guerra-Shinohara
Journal:  J Nutr       Date:  2017-07-19       Impact factor: 4.798

4.  A Common Polymorphism in HIBCH Influences Methylmalonic Acid Concentrations in Blood Independently of Cobalamin.

Authors:  Anne M Molloy; Faith Pangilinan; James L Mills; Barry Shane; Mary B O'Neill; David M McGaughey; Aneliya Velkova; Hatice Ozel Abaan; Per M Ueland; Helene McNulty; Mary Ward; J J Strain; Conal Cunningham; Miriam Casey; Cheryl D Cropp; Yoonhee Kim; Joan E Bailey-Wilson; Alexander F Wilson; Lawrence C Brody
Journal:  Am J Hum Genet       Date:  2016-04-28       Impact factor: 11.025

5.  Development and application of an alpha-face-specific radioimmunoassay for vitamin B12.

Authors:  D G Kennedy; W J Blanchflower; P B Young; W B Davidson
Journal:  Biol Trace Elem Res       Date:  1992-11       Impact factor: 3.738

6.  Association between Serum Unmetabolized Folic Acid Concentrations and Folic Acid from Fortified Foods.

Authors:  Cecília Zanin Palchetti; Clóvis Paniz; Eduardo de Carli; Dirce M Marchioni; Célia Colli; Josiane Steluti; Christine M Pfeiffer; Zia Fazili; Elvira Maria Guerra-Shinohara
Journal:  J Am Coll Nutr       Date:  2017-09-12       Impact factor: 3.169

7.  Higher maternal plasma folate but not vitamin B-12 concentrations during pregnancy are associated with better cognitive function scores in 9- to 10- year-old children in South India.

Authors:  Sargoor R Veena; Ghattu V Krishnaveni; Krishnamachari Srinivasan; Andrew K Wills; Sumithra Muthayya; Anura V Kurpad; Chittaranjan S Yajnik; Caroline H D Fall
Journal:  J Nutr       Date:  2010-03-24       Impact factor: 4.798

8.  Effect of physiological doses of oral vitamin B12 on plasma homocysteine: a randomized, placebo-controlled, double-blind trial in India.

Authors:  U S Deshmukh; C V Joglekar; H G Lubree; L V Ramdas; D S Bhat; S S Naik; P S Hardikar; D A Raut; T B Konde; A K Wills; A A Jackson; H Refsum; A S Nanivadekar; C H Fall; C S Yajnik
Journal:  Eur J Clin Nutr       Date:  2010-03-10       Impact factor: 4.016

9.  Low plasma vitamin B12 in pregnancy is associated with gestational 'diabesity' and later diabetes.

Authors:  G V Krishnaveni; J C Hill; S R Veena; D S Bhat; A K Wills; C L S Karat; C S Yajnik; C H D Fall
Journal:  Diabetologia       Date:  2009-08-26       Impact factor: 10.122

10.  Increases in plasma holotranscobalamin can be used to assess vitamin B-12 absorption in individuals with low plasma vitamin B-12.

Authors:  Dattatray S Bhat; Nileema V Thuse; Himangi G Lubree; Charudatta V Joglekar; Sadanand S Naik; Lalita V Ramdas; Carole Johnston; Helga Refsum; Caroline H Fall; Chittaranjan S Yajnik
Journal:  J Nutr       Date:  2009-09-23       Impact factor: 4.798

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