The Next Generation of Rice: Inter-Subspecific Indica-Japonica Hybrid Rice.

Rice (Oryza sativa) is an important food crop and has two subspecies, indica and japonica. Since the last century, four generations of rice varieties have been applied to rice production. Semi-dwarf rice, intra-subspecific hybrid rice, and inter-subspecific introgression rice were developed successively by genetic modification based on the first generation of tall rice. Each generation of rice has greater yield potential than the previous generation. Due to the stronger heterosis of indica-japonica hybrids, utilization of the inter-subspecific heterosis has long been of interest. However, indica-japonica hybrid sterility hinders the utilization of heterosis. In the past decades, indica-japonica hybrid sterility has been well understood. It is found that indica-japonica hybrid sterility is mainly controlled by six loci, S5, Sa, Sb, Sc, Sd, and Se. The indica-japonica hybrid sterility can be overcome by developing indica-compatible japonica lines (ICJLs) or wide-compatible indica lines (WCILs) using genes at the loci. With the understanding of the genetic and molecular basis of indica-japonica hybrid sterility and the development of molecular breeding technology, the development of indica-japonica hybrid rice has become possible. Recently, great progress has been made in breeding indica-japonica hybrid rice. Therefore, the indica-japonica hybrid rice will be the next generation of rice. It is expected that the indica-japonica hybrid rice will be widely applied in rice production in the near future.

Introduction

Rice (O. sativa) is the best-known cultivated crop, providing staple food for more than half of the world’s population (Fukagawa and Ziska, 2019). The domestication of cultivated rice began about 10,000 years ago. During domestication, the cultivated rice differentiated into two varietal groups. Since the Han dynasty, the Chinese have recognized two rice varietal groups, Hsien (Xian) and Keng (Geng) (Ting, 1949; Wang et al., 2018). In the last century, Kato et al. (1928) divided O. sativa into two subspecies, indica and japonica. Ting (1949, 1957) named the two subspecies as hsien and keng. Recently, the two subspecies are considered to be Xian/Indica (XI) and Geng/Japonica (GJ) (Wang et al., 2018). The two subspecies of cultivated rice are two different ecological types, where indica rice is suitable for growing in tropical and subtropical areas at low latitudes and low altitudes, while japonica rice is suitable for growing in temperate areas at high latitudes or high altitudes (Chang, 1976; Khush, 1997).

Rice is a self-pollinating plant. The heterosis of self-pollinating plants is usually considered to be weak. In the 1970s, indica hybrid rice was successfully applied in China. Since the 1980s, indica hybrid rice has been widely planted in China, accounting for more than 50% of the rice planting area (Cheng et al., 2007). The indica hybrid rice shows strong heterosis, which plays an important role in increasing rice yield (Yuan and Virmani, 1988; Zhang et al., 2021). At the same time, japonica hybrid rice has also been successfully developed, and a large number of japonica hybrid varieties have been released (Li and Wu, 1991; Zheng et al., 2020). It is believed that inter-subspecific hybrids have stronger heterosis than intra-subspecific hybrids (Fu et al., 2014; Birchler, 2015). Rice inter-subspecific heterosis has long been attempted, but the sterility of inter-subspecific hybrids has hindered the utilization of heterosis (Cheng et al., 2007; Zhang, 2020). Therefore, the key to utilization of inter-subspecific heterosis is to overcome the hybrid sterility.

The Five Generations of Rice

Although domestication of the cultivated rice started about 10,000 years ago, purposeful genetic improvement of varieties began in the last century. According to the genetic basis of varieties, the cultivated rice can be divided into five generations (Zhang, 2019, 2020).

The first generation (1G) of rice is tall rice. Since rice was cultivated about 10,000 years ago, the cultivated rice had been tall rice. Tall rice with high stalk was easy to cover the weeds in the field. Before the 1960s, tall rice was suitable for cultivating without chemical fertilizer. Tall rice had many landraces, which were the result of local intuitive selection by farmers for a long time (Zeven, 1998). As the first generation of rice, tall varieties have become an important genetic resource for rice breeding.

The second generation (2G) of rice is semi-dwarf rice. Around the 1950s, farmers began using chemical fertilizer. The yield of tall rice was greatly improved, but with it came the lodging. From lodging tall varieties, semi-dwarf mutants were selected as new varieties. In 1956, for example, two farmers of Guangdong province of China selected a semi-dwarf mutant from the field of lodging tall rice variety Nan-te 16. This semi-dwarf mutant became a new semi-dwarf variety Ai-jiao-nan-te, which was soon widely planted in southern China (Hu, 1965). At the same time, some semi-dwarf germplasm resources were selected to develop new semi-dwarf varieties by hybridization breeding. In 1956, for example, a breeding team of Guangdong Academy of Agricultural Sciences of China selected Ai-zi-zhan as a semi-dwarf parent and developed new semi-dwarf varieties Guang-chang-ai in 1959, Zhen-zhu-ai in 1961, and Guang-liu-ai 4 in 1966 (Guangdong Academy of Agricultural Sciences, 1966). Meanwhile, a semi-dwarf variety with high yield potential, IR8, was released by International Rice Research Institute (IRRI) in 1966 (Peng et al., 1994; Khush, 2001). The semi-dwarf varieties were bred by incorporating a recessive dwarf gene, sd1, to reduce plant height (Suh and Hue, 1978; Khush, 2001).

The third generation (3G) of rice is intra-subspecific hybrid rice, including indica hybrid rice and japonica hybrid rice. In the 1970s, indica hybrid rice was developed in China by a group of scientists led by Longping Yuan. Since then, the indica hybrid rice has been rapidly applied to production because of its strong heterosis (Yuan and Virmani, 1988). Meanwhile, japonica hybrid rice has also been developed and applied in rice production (Shiniyo, 1969; Li and Wu, 1991; Zheng et al., 2020).

The fourth generation (4G) of rice is inter-subspecific introgression rice, including japonica-introgressive indica rice and indica-introgressive japonica rice. Since the 1970s, restorer genes have been transferred from indica to japonica to develop japonica restorer lines for japonica hybrid rice. In the 1980s, the finding of S5-n gene (Ikehashi and Araki, 1986) and the “new plant type” program of IRRI (Peng et al., 2008) promoted the hybridization breeding between indica and japonica rice. Since then, more and more inter-subspecific introgression varieties have been developed and applied in rice production (Cheng et al., 2007; Ma et al., 2007; Lin et al., 2016).

The fifth generation (5G) of rice is inter-subspecific indica-japonica hybrid rice. The next generation rice has heterosis between indica and japonica subspecies, which will greatly improve the yield potential (Zhang, 2020). It is worth noting that many so-called indica-japonica hybrid varieties released are actually inter-subspecific introgression varieties that are 4G rice (Ma et al., 2007; Lin et al., 2016; Zhu et al., 2020).

Since the second half of the last century, rice breeding has developed rapidly. With the utilization of new genetic resources and new breeding techniques, the genetic basis of varieties has changed more and more. The yield potential of each generation of varieties has been greatly improved.

Genetic Basis of Indica-Japonica Hybrid Sterility

Reproductive isolation usually appears in inter-specific and inter-subspecific hybrids of plants. Reproductive isolation can occur at the prezygotic and postzygotic stages. Postzygotic reproductive isolation usually shows hybrid lethality, hybrid necrosis/weakness and hybrid sterility (Baack et al., 2015; Ouyang and Zhang, 2018). In the past decades, about 50 loci related to reproductive isolation of the genus Oryza have been identified (Ouyang and Zhang, 2013, 2018; Guo et al., 2016; Li et al., 2020). In indica-japonica crosses, reproductive isolation usually shows hybrid sterility. Among the loci for reproductive isolation of Oryza, only some of the loci are responsible for the hybrid sterility of indica-japonica crosses (Zhang, 2020).

In indica-japonica hybrids, female or embryo sac sterility is controlled by the S5 locus. At the locus, indica varieties usually have S5-i allele, while japonica varieties usually have S5-j allele. The interaction of S5-i and S5-j in indica-japonica hybrids causes the abortion of female gametes with the S5-j allele (Ikehashi and Araki, 1986). By genetic mapping, the S5 locus was located on chromosome 6 (Ikehashi and Araki, 1986; Yanagihara et al., 1995). Furthermore, the S5 gene was cloned and functionally analyzed (Chen et al., 2008; Yang et al., 2012). In the hybrids of wide indica-japonica crosses, female sterility is usually under the control of the S5 locus (Ikehashi and Araki, 1986; Song et al., 2005).

For male or pollen sterility of indica-japonica hybrids, five loci, Sa, Sb, Sc, Sd, and Se, were identified in wide indica-japonica crosses. At the loci, indica varieties usually have S-i allele, while japonica varieties usually have S-j allele. In indica-japonica hybrids, the interaction between S-i and S-j at the loci leads to the abortion of male gametes with the S-j allele. The male sterility shows two types of abortive pollens. The empty abortive pollen is caused by the Sa locus, while the stained abortive pollen is caused by the Sb, Sc, Sd, and Se loci. The degree of pollen sterility in indica-japonica hybrids depends on the number of heterozygous loci (Zhang and Lu, 1989, 1993, 1996; Zhang et al., 1993, 1994). By molecular mapping, the Sa, Sb, Sc, Sd, and Se loci were located on chromosomes 1, 5, 3, 1, and 12, respectively (Zhuang et al., 1999, 2002; Zhang and Zhang, 2001; Su and Liu, 2003; Yang et al., 2004; Li et al., 2006, 2008; Zhu et al., 2008). Furthermore, the Sa and Sc genes have been cloned and functionally analyzed (Long et al., 2008; Shen et al., 2017; Xie et al., 2017). The genes for hybrid sterility S24, S35, and S25 (S36) are found to be located in the same chromosomal regions as Sb, Sd, and Se, respectively, which may be the same loci (Kubo and Yoshimura, 2001; Wen et al., 2007; Kubo et al., 2008; Zhao et al., 2011). Therefore, the male sterility of indica-japonica hybrids is usually under the control of the five loci (Zhang, 2020).

Neutral (n) allele is usually found at the loci for hybrid sterility in plants. When two alleles of a locus interact to cause sterility, there may be a third allele, n allele, at the locus, whose interaction with other two alleles can’t cause sterility (Rich, 1966). At S5 locus, some tropical japonica accessions carry S5-n allele except indica varieties having S5-i and japonica varieties having S5-j (Ikehashi and Araki, 1986). At the Sa, Sb, Sc, Sd, and Se loci, not only S-i, S-j, and S-n alleles can be divided, but the effects of alleles from different donors also vary quantitatively, resulting in the continuous variation of pollen sterility at a single locus (Zhang et al., 1993). The molecular basis of neutral alleles has been revealed by the cloned genes of S5 (Chen et al., 2008; Yang et al., 2012), Sa (Long et al., 2008; Xie et al., 2017), and Sc (Shen et al., 2017).

Summarily, six loci of hybrid sterility are usually found in indica-japonica crosses, S5 for female sterility, and Sa, Sb, Sc, Sd, and Se for male sterility (Table 1 and Figure 1A). Generally, indica varieties have S-i allele, japonica varieties have S-j allele, while some accessions have S-n allele at these loci. The genic model of the loci is the one-locus sporo-gametophytic interaction model. In indica-japonica hybrids, the allelic interaction of S-i and S-j causes the abortion of female gametes carrying the S-j allele of S5 locus, and the abortion of male gametes carrying the S-j allele of Sa, Sb, Sc, Sd, and Se loci, resulting in hybrid sterility (Figure 1B). In contrast, the interaction of S-n with S-i or S-j can’t cause the abortion of any gametes (Ikehashi and Araki, 1986; Zhang, 2020). The understanding of the genetic basis of indica-japonica hybrid sterility has laid the foundation for overcoming the hybrid sterility.

TABLE 1

The loci for indica-japonica hybrid sterility.

Sterility	Locus	Chr.	Molecular mechanism	References
				Identification	Molecular mapping	Cloning and functional analysis
Female	S5	6	A killer-protector system encoded by three tightly linked genes	Ikehashi and Araki, 1986	Yanagihara et al., 1995; Ji et al., 2005; Qiu et al., 2005	Chen et al., 2008; Yang et al., 2012
Male	Sa	1	A two-gene/three component interaction model	Zhang and Lu, 1989, 1993; Zhang et al., 1993, 1994	Zhuang et al., 1999; Su and Liu, 2003	Long et al., 2008; Xie et al., 2017
	Sb	5			Zhuang et al., 2002; Li et al., 2006
	Sc	3	A model of gene dosage- dependent hybrid male sterility		Zhang and Zhang, 2001; Yang et al., 2004	Shen et al., 2017
	Sd	1			Li et al., 2008
	Se	12			Zhu et al., 2008

FIGURE 1

Strategies for overcoming indica-japonica hybrid sterility. (A) Chromosomal location of the S5, Sa, Sb, Sc, Sd, and Se loci for indica-japonica hybrid sterility. (B) Hybrid of indica/japonica is sterile, which is caused by the interaction between S-i and S-j at the S5, Sa, Sb, Sc, Sd, and Se loci. (C) Hybrid of indica/ICJL is fertile due to ICJL carrying S-i at the S5, Sa, Sb, Sc, Sd, and Se loci. (D) Hybrid of WCIL/japonica is fertile due to WCIL carrying S-n at the S5, Sa, Sb, Sc, Sd, and Se loci. ICJL, indica-compatible japonica line; WCIL, wide-compatible indica line.

Strategies for Overcoming Indica-Japonica Hybrid Sterility

Based on the genetic basis of indica-japonica hybrid sterility, two types of breeding lines can be developed to overcome the hybrid sterility. They are indica-compatible japonica lines (ICJLs) (Zhang et al., 1994; Zhang and Lu, 1999) and wide-compatibility lines (WCLs). The breeding lines can be used to develop indica-japonica hybrid rice without hybrid sterility (Zhang, 2020).

The ICJLs can be developed by transferring S-i alleles at the S5, Sa, Sb, Sc, Sd, and Se loci from indica to japonica by backcrossing (Figure 1C). For example, a set of Taichung 65 (T65) isogenic F1-sterile lines (TISLs) having S-i alleles at the Sa, Sb, Sc, Sd, and Se loci were developed using a set of indica varieties as S-i donors in the genetic background of T65, a japonica variety. Then, the S-i alleles of these loci were pyramided together by crossing the TISLs. The hybrid pollen fertility of pyramiding lines with different genotypes at these loci were tested with indica and japonica testers. The results showed that as the number of S-i alleles at these loci in the pyramiding lines increased, the pollen fertility of hybrids with indica testers increased, while that of hybrids with japonica testers decreased (Zhang and Lu, 1996; Guo et al., 2016). Furthermore, by pyramiding the S5-n allele in the pyramiding lines with the S-i alleles of the Sb, Sc, Sd, and Se loci, several ICJLs with Sb-i, Sc-i, Sd-i, Se-i, and S5-n alleles in japonica genetic background were developed. The ICJLs showed normal or near normal pollen fertility and spikelet fertility in their hybrids with indica testers, but serious pollen sterility and spikelet sterility in the hybrids with japonica testers. Therefore, the indica-japonica hybrid sterility can be overcome in the crosses of ICJLs with indica varieties (Guo et al., 2016).

The WCLs can be developed by using the S-n alleles of six loci. WCLs with indica genetic background are wide-compatible indica lines (WCILs), and WCLs with japonica genetic background are wide-compatible japonica lines (WCJLs). The indica-japonica hybrid rice can be developed by using WCILs crossed with japonica lines, or by using WCJLs crossed with indica lines (Figure 1D).

Discussion

Since the 1970s, the intra-subspecific hybrid rice, including indica hybrid rice and japonica hybrid rice, has been developed. The cytoplasmic male sterility (CMS) system is a three-line system including CMS line, maintainer line and restorer line (Yuan and Virmani, 1988; Chen and Liu, 2014). The photoperiod/thermosensitive genic male-sterility (PTGMS) system is a two-line system including PTGMS line and restorer line (Shi, 1985; Ding et al., 2012; Zhou et al., 2012). The CMS system and the PTGMS system have been widely utilized in hybrid rice production. The application of genic male-sterility (GMS) materials in hybrid rice is the third-generation hybrid rice breeding technology (Deng et al., 2013; Wang and Deng, 2018; Song et al., 2021). A large number of breeding lines have been developed by the three generations of hybrid rice breeding technology. These techniques and breeding lines can be used to develop not only intra-subspecific hybrid rice but also inter-subspecific hybrid rice. For examples, the CMS lines, PTGMS lines, and GMS lines are male sterility lines (MSLs) that can also be used for the breeding of inter-subspecific hybrid rice. The restorer lines of CMS system, PTGMS system and GMS system can be used for the breeding of inter-subspecific hybrid rice after improving their compatibility. Thus, the breeding of intra-subspecific hybrid rice provides available breeding lines for the development of inter-subspecific hybrid rice.

Compared with intra-subspecific hybrid rice and inter-subspecific introgression rice, the development of inter-subspecific hybrid rice will face greater challenges. Firstly, the overcoming of indica-japonica hybrid sterility requires to pyramid multiple genes for compatibility. Secondly, male sterility and fertility restoration should be considered in hybrid rice breeding. Third, there may be some problems caused by the remote genetic backgrounds of the two subspecies (Lin et al., 2016). To meet the challenge, it is necessary to develop molecular breeding techniques. For the past two decades, we have been building a library of chromosome single-segment substitution lines (SSSLs) to construct a target chromosome-segment substitution platform for rice design (Zhang, 2021). The SSSL library was constructed by using forty-three accessions from seven species with AA genome as donors of chromosome segments in the genetic background of Huajingxian 74 (HJX74), an elite indica variety in South China. The HJX74-SSSL library consists of 2,360 SSSLs, which collects rich gene resources from donors with genetic diversity (Zhang et al., 2004; Xi et al., 2006; He et al., 2017; Zhao et al., 2019; Zhang, 2021). The HJX74-SSSLs have been used to detect QTLs for complex traits (Zhang et al., 2012; Yang et al., 2016, 2021a,2021b; Zhou et al., 2017; Tan et al., 2020, 2021, 2022; Pan et al., 2021), to clone genes of agronomic importance and to assess allelic variation (Wang et al., 2008, 2012, 2015; Teng et al., 2012; Sui et al., 2019; Zhang et al., 2020; Gao et al., 2021). Using the HJX74-SSSL library as platform for rice breeding by design, several CMS, maintainer and restorer lines have been developed (Dai et al., 2015, 2016; Luan et al., 2019). These results suggest that the target chromosome-segment substitution is an effective way to rice breeding by design (Zhang, 2021). Recently, the HJX74-SSSL library was used to develop WCILs. Through the restorer gene pyramiding, the WCILs will be developed into wide-compatible indica restorer lines (WCIRLs) as restorer lines of indica-japonica hybrid rice. Therefore, the target chromosome-segment substitution based on HJX74-SSSL platform provides technical support for the development of indica-japonica hybrid rice.

For a century, four generations of rice have provided a large number of elite varieties for rice production. With the changes of genetic basis, each generation of rice has greater yield potential than the previous generation. The application of new generation rice has greatly improved the productivity of modern rice. However, the intra-subspecific hybrid rice can only have intra-subspecific heterosis, and the inter-subspecific introgression rice can only utilize partial inter-subspecific heterosis. In comparison, the inter-subspecific indica-japonica hybrid rice can take advantage of complete inter-subspecific heterosis. Therefore, the utilization of heterosis between indica and japonica subspecies has been expected (Cheng et al., 2007; Zhang, 2020). With the understanding of the genetic and molecular basis of indica-japonica hybrid sterility and the development of molecular breeding techniques, it is now possible to develop indica-japonica hybrid rice. We are developing indica-japonica hybrid rice by crossing WCIRLs developed on the HJX74-SSSL platform with existing japonica CMS lines collected from japonica planting areas. Many indica-japonica hybrid rice combinations have been extensively tested in various rice planting areas of China. Our results showed that indica-japonica hybrid rice had stronger heterosis and higher yield potential. Therefore, indica-japonica hybrid rice will become the next generation of rice and will be widely applied in rice production in the near future.

Data Availability Statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Author Contributions

GZ wrote the manuscript independently.

Conflict of Interest

The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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