| Literature DB >> 35412279 |
J Tabitha Hees1, Angelika B Harbauer2.
Abstract
The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging.Entities:
Keywords: Cultured primary neurons; Imaging; MS2; PP7; RNA transport; Split-Venus; Transient transfection
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Year: 2022 PMID： 35412279 DOI： 10.1007/978-1-0716-1990-2_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745