Literature DB >> 35412251

Imaging Fluorescent Nuclear Pore Complex Proteins in C. elegans.

Courtney Lancaster1, Giulia Zavagno2, James Groombridge2, Adelaide Raimundo2, David Weinkove2, Tim Hawkins2, Joanne Robson2, Martin W Goldberg3.   

Abstract

C. elegans is a well-characterized and relatively simple model organism, making it attractive for studying nuclear pore complex proteins in cell and developmental biology. C. elegans is transparent and highly amendable to genetic manipulation. Therefore, it is possible to generate fluorescently tagged proteins and combine this with various light microscopy techniques to study protein behavior in space and time. Here, we provide protocols to prepare both fixed and live C. elegans for confocal and light sheet microscopy. This enables the analysis of nuclear pore complex proteins from embryonic stages to the aging adult.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  3D-SIM; C. elegans; Confocal; Fluorescence microscopy; Light sheet; Nuclear pore complex; Nucleus; Superresolution; fluorescent protein

Mesh：

Substances：

Year:  2022        PMID： 35412251     DOI： 10.1007/978-1-0716-2337-4_24

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

Review 1.  Cell Biology of the Caenorhabditis elegans Nucleus.

Authors:  Orna Cohen-Fix; Peter Askjaer
Journal:  Genetics       Date:  2017-01       Impact factor: 4.562

2.  Efficient Generation of Endogenous Fluorescent Reporters by Nested CRISPR in Caenorhabditis elegans.

Authors:  Jeremy Vicencio; Carmen Martínez-Fernández; Xènia Serrat; Julián Cerón
Journal:  Genetics       Date:  2019-01-29       Impact factor: 4.562

3.  Post-embryonic cell lineages of the nematode, Caenorhabditis elegans.

Authors:  J E Sulston; H R Horvitz
Journal:  Dev Biol       Date:  1977-03       Impact factor: 3.582

4.  The embryonic cell lineage of the nematode Caenorhabditis elegans.

Authors:  J E Sulston; E Schierenberg; J G White; J N Thomson
Journal:  Dev Biol       Date:  1983-11       Impact factor: 3.582

5.  C. elegans nuclear envelope proteins emerin, MAN1, lamin, and nucleoporins reveal unique timing of nuclear envelope breakdown during mitosis.

Authors:  K K Lee; Y Gruenbaum; P Spann; J Liu; K L Wilson
Journal:  Mol Biol Cell       Date:  2000-09       Impact factor: 4.138

6.  MEL-28, a novel nuclear-envelope and kinetochore protein essential for zygotic nuclear-envelope assembly in C. elegans.

Authors:  Vincent Galy; Peter Askjaer; Cerstin Franz; Carmen López-Iglesias; Iain W Mattaj
Journal:  Curr Biol       Date:  2006-09-05       Impact factor: 10.834

7.  Early embryonic requirement for nucleoporin Nup35/NPP-19 in nuclear assembly.

Authors:  Eduardo Ródenas; Elke P F Klerkx; Cristina Ayuso; Anjon Audhya; Peter Askjaer
Journal:  Dev Biol       Date:  2008-12-30       Impact factor: 3.582

8.  Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo.

Authors:  Vincent Galy; Iain W Mattaj; Peter Askjaer
Journal:  Mol Biol Cell       Date:  2003-08-22       Impact factor: 4.138

9.  Age-dependent deterioration of nuclear pore complexes causes a loss of nuclear integrity in postmitotic cells.

Authors:  Maximiliano A D'Angelo; Marcela Raices; Siler H Panowski; Martin W Hetzer
Journal:  Cell       Date:  2009-01-23       Impact factor: 41.582

10.  Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation.

Authors:  Georgina Gómez-Saldivar; Anita Fernandez; Yasuhiro Hirano; Michael Mauro; Allison Lai; Cristina Ayuso; Tokuko Haraguchi; Yasushi Hiraoka; Fabio Piano; Peter Askjaer
Journal:  PLoS Genet       Date:  2016-06-24       Impact factor: 5.917
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