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Literature DB >> 35412230

Nuclear Pore Complex Assembly Using Xenopus Egg Extract.

Abstract

Xenopus egg extract is a powerful tool for the in vitro investigation of complex cellular mechanisms. Here we describe how to obtain and employ interphase Xenopus egg extract to study nuclear pore complex assembly and how to analyze the process using Western blot or immunofluorescence based assays. The function of proteins can be conveniently assayed by high-efficient antibody mediated depletion.

Entities: Chemical

Keywords: Annulate lamellae; Immunofluorescence; Interphase egg extract; Nuclear assembly; Nuclear pore complex; Western blot; Xenopus laevis

Mesh：

Year: 2022 PMID： 35412230 DOI： 10.1007/978-1-0716-2337-4_3

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

13 in total

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Journal: Nat Commun Date: 2014 Impact factor: 14.919

Nuclear Pore Complex Assembly Using Xenopus Egg Extract.

Review 1. Assembly of nuclear pore complexes in Xenopus egg extract.

2. Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.

3. RuvB-like ATPases function in chromatin decondensation at the end of mitosis.

4. In vitro activation of human sperm induced by amphibian egg extract.

5. The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation.

6. Chromatin decondensation and DNA synthesis in human sperm activated in vitro by using Xenopus laevis egg extracts.

7. The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex.

8. Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.

9. Requirement for MAPK activation for normal mitotic progression in Xenopus egg extracts.

10. Dimerization and direct membrane interaction of Nup53 contribute to nuclear pore complex assembly.