Literature DB >> 35412229

Transformation of Chaetomium thermophilum and Affinity Purification of Native Thermostable Protein Complexes.

Nikola Kellner1, Ed Hurt2.   

Abstract

Chaetomium thermophilum, a eukaryotic thermophile, is an aspiring organism holding great potential for various biochemical and biotechnological applications. Prerequisite for genetic manipulation is a reliable transformation system for target genes combined with selection markers operating at the high growth temperatures of the fungus. Here, we present a detailed protocol for Chaetomium thermophilum protoplast transformation to allow stable chromosomal integration of constructs into its genome, rendering this eukaryotic thermophile a valuable resource for affinity purification of native thermostable protein complexes, like nuclear pore subcomplexes.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Affinity purification; Chaetomium thermophilum; Genetic transformation; Nuclear pore complex (NPC); Thermophile

Mesh：

Substances：

Year:  2022        PMID： 35412229     DOI： 10.1007/978-1-0716-2337-4_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  25 in total

1.  Architecture of the fungal nuclear pore inner ring complex.

Authors:  Tobias Stuwe; Christopher J Bley; Karsten Thierbach; Stefan Petrovic; Sandra Schilbach; Daniel J Mayo; Thibaud Perriches; Emily J Rundlet; Young E Jeon; Leslie N Collins; Ferdinand M Huber; Daniel H Lin; Marcin Paduch; Akiko Koide; Vincent Lu; Jessica Fischer; Ed Hurt; Shohei Koide; Anthony A Kossiakoff; André Hoelz
Journal:  Science       Date:  2015-08-27       Impact factor: 47.728

2.  Efficiency of new fungal cellulase systems in boosting enzymatic degradation of barley straw lignocellulose.

Authors:  Lisa Rosgaard; Sven Pedersen; Joel R Cherry; Paul Harris; Anne S Meyer
Journal:  Biotechnol Prog       Date:  2006 Mar-Apr

3.  Protein interfaces of the conserved Nup84 complex from Chaetomium thermophilum shown by crosslinking mass spectrometry and electron microscopy.

Authors:  Karsten Thierbach; Alexander von Appen; Matthias Thoms; Martin Beck; Dirk Flemming; Ed Hurt
Journal:  Structure       Date:  2013-08-15       Impact factor: 5.006

4.  Two novel thermostable chitinase genes from thermophilic fungi: cloning, expression and characterization.

Authors:  An-Na Li; Kai Yu; Hai-Quan Liu; Jie Zhang; Hua Li; Duo-Chuan Li
Journal:  Bioresour Technol       Date:  2010-03-12       Impact factor: 9.642

5.  Interaction network of the ribosome assembly machinery from a eukaryotic thermophile.

Authors:  Jochen Baßler; Yasar Luqman Ahmed; Martina Kallas; Markus Kornprobst; Fabiola R Calviño; Marén Gnädig; Matthias Thoms; Gunter Stier; Sherif Ismail; Satyavati Kharde; Nestor Castillo; Sabine Griesel; Sonja Bastuck; Bettina Bradatsch; Emma Thomson; Dirk Flemming; Irmgard Sinning; Ed Hurt
Journal:  Protein Sci       Date:  2017-01-14       Impact factor: 6.725

6.  Insight into structure and assembly of the nuclear pore complex by utilizing the genome of a eukaryotic thermophile.

Authors:  Stefan Amlacher; Phillip Sarges; Dirk Flemming; Vera van Noort; Ruth Kunze; Damien P Devos; Manimozhiyan Arumugam; Peer Bork; Ed Hurt
Journal:  Cell       Date:  2011-07-22       Impact factor: 41.582

7.  Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases.

Authors:  Sanni P Voutilainen; Terhi Puranen; Matti Siika-Aho; Arja Lappalainen; Marika Alapuranen; Jarno Kallio; Satu Hooman; Liisa Viikari; Jari Vehmaanperä; Anu Koivula
Journal:  Biotechnol Bioeng       Date:  2008-10-15       Impact factor: 4.530

8.  Purification and characterization of two thermostable proteases from the thermophilic fungus Chaetomium thermophilum.

Authors:  An-Na Li; Ai-Yun Ding; Jing Chen; Shou-An Liu; Ming Zhang; Duo-Chuan Li
Journal:  J Microbiol Biotechnol       Date:  2007-04       Impact factor: 2.351

9.  An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum.

Authors:  Thomas Bock; Wei-Hua Chen; Alessandro Ori; Nayab Malik; Noella Silva-Martin; Jaime Huerta-Cepas; Sean T Powell; Panagiotis L Kastritis; Georgy Smyshlyaev; Ivana Vonkova; Joanna Kirkpatrick; Tobias Doerks; Leo Nesme; Jochen Baßler; Martin Kos; Ed Hurt; Teresa Carlomagno; Anne-Claude Gavin; Orsolya Barabas; Christoph W Müller; Vera van Noort; Martin Beck; Peer Bork
Journal:  Nucleic Acids Res       Date:  2014-11-14       Impact factor: 16.971

10.  Consistent mutational paths predict eukaryotic thermostability.

Authors:  Vera van Noort; Bettina Bradatsch; Manimozhiyan Arumugam; Stefan Amlacher; Gert Bange; Chris Creevey; Sebastian Falk; Daniel R Mende; Irmgard Sinning; Ed Hurt; Peer Bork
Journal:  BMC Evol Biol       Date:  2013-01-10       Impact factor: 3.260
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.