| Literature DB >> 35405431 |
Michael A Paley1, Brandi J Baker2, S Richard Dunham2, Nicole Linskey3, Claudia Cantoni2, Kenneth Lee2, Lynn M Hassman4, Jennifer Laurent3, Elisha D O Roberson5, David B Clifford2, Wayne M Yokoyama6.
Abstract
The tissue-specific drivers of neurosarcoidosis remain poorly defined. To identify cerebrospinal fluid (CSF) specific, antigen-driven T and B cell responses, we performed single-cell RNA sequencing of CSF and blood cells from neurosarcoid participants coupled to T and B cell receptor sequencing. In contrast to pulmonary sarcoidosis, which is driven by CD4 T cells, we found CD8 T cell clonal expansion enriched in the neurosarcoid CSF. These CSF-enriched CD8 T cells were composed of two subsets with differential expression of EBI2, CXCR3, and CXCR4. Lastly, our data suggest that IFNγ signaling may distinguish neurosarcoidosis from other neurological disorders.Entities:
Keywords: Interferon; Neurosarcoidosis; Sarcoidosis; Single-cell RNA sequencing; T cells; TCR sequencing
Mesh：
Year: 2022 PMID： 35405431 PMCID： PMC9338453 DOI： 10.1016/j.jneuroim.2022.577860
Source DB: PubMed Journal: J Neuroimmunol ISSN： 0165-5728 Impact factor: 3.221