Riikka E Laurila1, Tom O Böhling2, Carl P Blomqvist3,4, Christina Karlsson4, Erkki J Tukiainen5, Jussi Repo6, Mika M Sampo1. 1. Department of Pathology, HUSLAB and University of Helsinki, Helsinki, Finland. 2. University of Helsinki, Helsinki, Finland. 3. Comprehensive Cancer Center, Helsinki University Hospital and University of Helsinki, Helsinki, Finland. 4. Örebro University, School of Health sciences, Örebro, Sweden. 5. Department of Plastic Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland. 6. Department of Orthopedics and Traumatology, Tampere University Hospital and University of Tampere, Tampere, Finland.
Abstract
Background: Ki-67 is a widely used proliferation marker reflecting prognosis in various tumors. However, visual assessment and scoring of Ki-67 suffers from marked inter-observer and intra-observer variability. We aimed to assess the concordance of manual counting and automated image-analytic scoring methods for Ki-67 in synovial sarcoma. Patients and Methods: Tissue microarrays from 34 patients with synovial sarcoma were immunostained for Ki-67 and scored both visually and with 3DHistech QuantCenter. Results: The automated assessment of Ki-67 expression was in good agreement with the visually counted Ki-67 (r Pearson =0.96, p<0.001). In a Cox regression model automated [hazard ratio (HR)=1.047, p=0.024], but not visual (HR=1.063, p=0.053) assessment method associated high Ki-67 scores with worse overall survival. Conclusion: The automated Ki-67 assessment method appears to be comparable to the visual method in synovial sarcoma and had a significant association to overall survival. Copyright 2022, International Institute of Anticancer Research.
Background: Ki-67 is a widely used proliferation marker reflecting prognosis in various tumors. However, visual assessment and scoring of Ki-67 suffers from marked inter-observer and intra-observer variability. We aimed to assess the concordance of manual counting and automated image-analytic scoring methods for Ki-67 in synovial sarcoma. Patients and Methods: Tissue microarrays from 34 patients with synovial sarcoma were immunostained for Ki-67 and scored both visually and with 3DHistech QuantCenter. Results: The automated assessment of Ki-67 expression was in good agreement with the visually counted Ki-67 (r Pearson =0.96, p<0.001). In a Cox regression model automated [hazard ratio (HR)=1.047, p=0.024], but not visual (HR=1.063, p=0.053) assessment method associated high Ki-67 scores with worse overall survival. Conclusion: The automated Ki-67 assessment method appears to be comparable to the visual method in synovial sarcoma and had a significant association to overall survival. Copyright 2022, International Institute of Anticancer Research.
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